Aptamer-based sandwich-type biosensors

Seo, Ho Bin; Gu, Man Bock

doi:10.1186/s13036-017-0054-7

Cited by 117 publications

(54 citation statements)

References 42 publications

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“…The chromatographic strip assay method (Wang et al, 2011) utilising labled aptamer for rapid toxin detection. High sensitivity of toxin detection can be achived in a sandwich assay format, for example using a pair of aptamer and antibody specific to toxins (Costantini et al, 2016;Seo et al, 2017). ( Bonel et al, 2011;Rhouati et al,2013b ).…”

Section: Introductionmentioning

confidence: 99%

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Rubaye

Nabok

Catanante

et al. 2018

Sensors and Actuators B: Chemical

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The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant K D = 1.8. 10-8 Mol which is characteristic for highly specific aptamer/OTA binding.

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Section: Introductionmentioning

confidence: 99%

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Rubaye

Nabok

Catanante

et al. 2018

Sensors and Actuators B: Chemical

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“…In such familiar architectures which have already prospered in several platforms [128,[149][150][151][152], primary aptamers can be immobilized on FET channels to detect analytes (proteins, DNA, RNA, small substances, low-/un-charged molecules) before coupling with secondary aptamers for signal enhancement. This scheme potentially unblocks another approach for sensing heavy metal ions constituting a huge proportion of the biosensor market [105,[153][154][155].…”

Section: Aptamers As Bio-amplifiers For Fet Biosensorsmentioning

confidence: 99%

Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

Chen

2020

Molecules

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Aptamers, in sensing technology, are famous for their role as receptors in versatile applications due to their high specificity and selectivity to a wide range of targets including proteins, small molecules, oligonucleotides, metal ions, viruses, and cells. The outburst of field-effect transistors provides a label-free detection and ultra-sensitive technique with significantly improved results in terms of detection of substances. However, their combination in this field is challenged by several factors. Recent advances in the discovery of aptamers and studies of Field-Effect Transistor (FET) aptasensors overcome these limitations and potentially expand the dominance of aptamers in the biosensor market.

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“…In recent years, functional nucleic acids known as aptamers (DNA or RNA) have demonstrated the ability to potentially overcome the limitations associated with antibodies. [21][22][23][24][25][26] Owing to these attributes, aptamers are regarded as a strong chemical rival of antibodies, with an ability to replace antibodies in all possible diagnostic formats. 22,27 Furthermore, in the last few years, aptamers have demonstrated their utility in the detection of diverse analytes ranging from small molecules to antigens to enzymes to hormones and even whole bacterial or cancer cells.…”

Section: Introductionmentioning

confidence: 99%

<p>Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis</p>

Das¹,

Dhiman²,

Mishra³

et al. 2019

IJN

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Background: Tuberculous meningitis (TBM) is the most devastating manifestation of extrapulmonary tuberculosis. About 33% of TBM patients die due to very late diagnosis of the disease. Conventional diagnostic methods based on signs and symptoms, cerebrospinal fluid (CSF) smear microscopy or liquid culture suffer from either poor sensitivity or long turnaround time (up to 8 weeks). Therefore, in order to manage the disease efficiently, there is an urgent and unmet need for a rapid and reliable diagnostic test. Methods: In the current study, to address the diagnostic challenge of TBM, a highly rapid and sensitive structural switching electrochemical aptasensor was developed by combining the electrochemical property of methylene blue (MB) with the molecular recognition ability of a ssDNA aptamer. To demonstrate the clinical diagnostic utility of the developed aptasensor, a blinded study was performed on 81 archived CSF specimens using differential pulse voltammetry. Results: The electrochemical aptasensor developed in the current study can detect as low as 10 pg HspX in CSF background and yields a highly discriminatory response (P,0.0001) for TBM and not-TBM categories with ~95% sensitivity and ~97.5% specificity and has the ability to deliver sample-to-answer in #30 minutes. Conclusion: In summary, we demonstrate a new aptamer-based electrochemical biosensing strategy by exploiting the target-induced structural switching of H63 SL-2 M6 aptamer and electroactivity of aptamer-tagged MB for the detection of HspX in CSF samples for the diagnosis of TBM. Further, the clinical utility of this sensor could be extended for the diagnosis of other forms of tuberculosis in the near future.

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Aptamer-based sandwich-type biosensors

Cited by 117 publications

References 42 publications

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

<p>Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis</p>

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