Aptamer-Based Silver Nanoparticles Used for Intracellular Protein Imaging and Single Nanoparticle Spectral Analysis

Chen, Li Qiang; Xiao, Shu-Hua; Li, Peng; Wu, Tong; Ling, Jian; Li, Yuan Fang; Huang, Cheng

doi:10.1021/jp9104618

Cited by 88 publications

(63 citation statements)

References 29 publications

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“…The uptake of Au NPs via caveolinmediated endocytosis has already been observed in previous studies [23,51,57,58]. These studies, however, used different models and differently modified and sized particles.…”

Section: Cellular Np Uptakementioning

confidence: 54%

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

et al. 2014

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Aim:We examined cellular uptake mechanisms of fluorescently labeled polymer-coated gold nanoparticles (NPs) under different biological conditions by two quantitative, microscopic approaches. Materials & methods: Uptake mechanisms were evaluated using endocytotic inhibitors that were tested for specificity and cytotoxicity. Cellular uptake of gold NPs was analyzed either by laser scanning microscopy or transmission electron microscopy, and quantified by means of stereology using cells from the same experiment. Results: Optimal inhibitor conditions were only achieved with chlorpromazine (clathrin-mediated endocytosis) and methyl--cyclodextrin (caveolinmediated endocytosis). A significant methyl--cyclodextrin-mediated inhibition (63-69%) and chlorpromazine-mediated increase (43-98%) of intracellular NPs was demonstrated with both imaging techniques, suggesting a predominant uptake via caveolin-medicated endocytois. Transmission electron microscopy imaging revealed more than 95% of NPs localized in intracellular vesicles and approximately 150-times more NP events/cell were detected than by laser scanning microscopy. Conclusion: We emphasize the importance of studying NP-cell interactions under controlled experimental conditions and at adequate microscopic resolution in combination with stereology. Keywords:In recent years, various types of nanoparticles (NPs; <100 nm in all three dimensions, ISO TS 27687:2008) have been designed for potential biomedical and pharmaceutical applications, such as vehicles for targeted drug delivery, nucleic acids, biomedical imaging or biosensing [1][2][3]. Therefore, an increasing number of research studies have focused on the interaction of NPs with biocellular systems [4,5]. To analyze different types of NP-cell interactions, such as cellular NP uptake, it is essential to have valuable techniques for visualization and quantification, which are mainly based on spectroscopy or microscopy [6].The method of choice for the intracellular detection of NPs depends on the characteristics of the particles (fluorescence, size, and structure or electron density) and on the cellular structure of interest. Advantages of spectroscopy and different microscopic techniques have been reviewed in detail elsewhere [6,7]. In brief, spectroscopy such as inductively coupled plasma-mass spectroscopy [8] allows the quantification and detection of chemical NP elements within the cell, but does not provide any further information on the NP location within the cell. Fluorescence or laser scanning microscopy (LSM) allows real-time analysis or co-localization studies with fluorescently labeled structures [9]. Transmission electron microscopy (TEM) provides the highest resolution and allows quantification of NPs in sub cellular structures [10].

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Section: Cellular Np Uptakementioning

confidence: 54%

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

et al. 2014

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“…Besides the abovementioned studies where aptamers were labeled with various tags for molecular imaging applications with each modality, aptamers have also been explored for several other imaging techniques such as computed tomography (CT), surface plasmon resonance (SPR), dark-field light scattering microscopy and transmission electron microscopy (TEM) [96], as well as surface-enhanced Raman spectroscopy (SERS) imaging [97]. …”

Section: Other Imaging Techniquesmentioning

confidence: 99%

Molecular Imaging with Nucleic Acid Aptamers

Hong¹,

Goel²,

Zhang³

et al. 2011

CMC

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With many desirable properties such as ease of synthesis, small size, lack of immunogenicity, and versatile chemistry, aptamers represent a class of targeting ligands that possess tremendous potential in molecular imaging applications. Non-invasive imaging of various disease markers with aptamer-based probes has many potential clinical applications such as lesion detection, patient stratification, treatment monitoring, etc. In this review, we will summarize the current status of molecular imaging with aptamer-based probes. First, fluorescence imaging will be described which include both direct targeting and activatable probes. Next, we discuss molecular magnetic resonance imaging and targeted ultrasound investigations using aptamer-based agents. Radionuclide-based imaging techniques (single-photon emission computed tomography and positron emission tomography) will be summarized as well. In addition, aptamers have also been labeled with various tags for computed tomography, surface plasmon resonance, dark-field light scattering microscopy, transmission electron microscopy, and surface-enhanced Raman spectroscopy imaging. Among all molecular imaging modalities, no single modality is perfect and sufficient to obtain all the necessary information for a particular question. Thus, a multimodality probe has also been constructed for concurrent fluorescence, gamma camera, and magnetic resonance imaging in vivo. Although the future of aptamer-based molecular imaging is becoming increasingly bright and many proof-of-principle studies have already been reported, much future effort needs to be directed towards the development of clinically translatable aptamer-based imaging agents which will eventually benefit patients.

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“…Since the discovery of aptamer, it has been widely used as an excellent ligand to recognize prions [12], and many high sensitive detection methods have been achieved [13][14]. Aptamer is a type of DNA or RNA oligonucleotides that can bind to various molecular targets such as small molecules, proteins, nucleic acids even cells, tissues and organisms [15].…”

Section: Introductionmentioning

confidence: 99%

Coomassie brilliant blue R-250 as a new surface-enhanced Raman scattering probe for prion protein through a dual-aptamer mechanism

Liu

Zhan

et al. 2015

Talanta

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Aptamer-Based Silver Nanoparticles Used for Intracellular Protein Imaging and Single Nanoparticle Spectral Analysis

Cited by 88 publications

References 29 publications

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

Molecular Imaging with Nucleic Acid Aptamers

Coomassie brilliant blue R-250 as a new surface-enhanced Raman scattering probe for prion protein through a dual-aptamer mechanism

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