Aptamer Conformation-Cooperated Enzyme-Assisted Surface-Enhanced Raman Scattering Enabling Ultrasensitive Detection of Cell Surface Protein Biomarkers in Blood Samples

Li, Yingying; Fang, Qianqian; Miao, Xinxing; Zhang, Xiuyan; Zhao, Yun; Yan, Jun; Zhang, Yiqiu; Wu, Renfei; Nie, Baoqing; Hirtz, Michael; Liu, Jian

doi:10.1021/acssensors.9b00604

Cited by 23 publications

(14 citation statements)

References 50 publications

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“…With the distinct advantages of low background, insensitivity to photobleaching or quenching, single-molecule sensitivity and fingerprint-like spectra, together with the controllable assembly of nanoparticles with functional nucleic acids, SERS can also be used for detection methods in nucleic acidbased bioanalysis. Examples include quantifications of metal ions and proteins in vitro as well as tumor markers and metabolites in situ [310,311]. Overall, with the development of various technological platforms, the optical reporting strategies will continually play important roles in nucleic acid-based bioanalysis.…”

Section: Signal Reporting Strategiesmentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

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Section: Signal Reporting Strategiesmentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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“…The LOD of 0.26 pg/mL for the CEA antigen was comparable vs. the Cell Biolabs ELISA kit. Sensitive and specific detection in the solution and directly on the surface of live CCRF-CEM cells with overexpressed PTK7 was demonstrated by Li and coworkers using surface-enhanced Raman scattering [2]. They developed the synergistic combination of the conformational alteration ofthe anglerfish aptamer triggered by the recognition of thePTK7 and Exo III enzyme-assistednucleic acid amplification.…”

Section: Sensitivity Of the Aptasensormentioning

confidence: 99%

“…The standard and representative assays for the detection of PTK7 protein are real-time quantitative PCR, flow cytometry, and ELISA kit [2]. These methods are, however, time-consuming, multi-step, and involve expensive tests.…”

Section: Introductionmentioning

confidence: 99%

The Development of Electrochemical Aptasensor Based on DNA Aptamers Modified by Redox Markers for Detection of Leukemia Jurkat Cells

Babelova

Slabý

Hianik

2020

The 1st International Electronic Conference on Biosensors

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Oncological diseases belong to the most serious illnesses with high mortality. The most common cancer in children is acute lymphoblastic leukemia (ALL). It is important to develop diagnostic methods that will be able to detect this disease in early stage. One of the possible options can be non-invasive diagnostics using the biosensors based on nucleic acid aptamers. Aptamer recognizes the surface markers on the membrane of cancer cells with the high binding affinity. Biosensors based on aptamers with redox markers are among the most sensitive experimental tools of this type. We developed and optimized the redox-labeled electrochemical aptasensors for the detection of Jurkat leukemia cells. The aptamers specific to the protein tyrosine kinase 7 (PTK7), which is important membrane protein cancer marker that is overexpressed in Jurkat cells were used. We compared the sensitivity of aptasensors for aptamers modified either by methylene blue (MB) and ferrocene carboxylic acid (Fc), respectively. Both aptasensors were tested in the presence of Jurkat cells at concentration range 50–5000 cells/mL using differential pulse voltammetry. In both cases the comparable sensitivity was obtained with limit of detection: 37 ± 6 cells/mL for Fc-labeled aptamers and 38 ± 8 cells/mL for MB-labeled aptamers based on 3.3S/N (signal to noise) rule. The interaction of the sensing surface with control U266 cells was less significant.

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“…11,12 At present, various aptasensors have been reported to detect mycotoxins based on electrochemistry, 13 fluorescence, 14 and chemiluminesence. 15 However, aptamer-based sensors always require chemical labeling, which brings about complexity and high cost and limit its practical use. Therefore, label-free detection method for mycotoxins is highly needed.…”

mentioning

confidence: 99%

“…The emerging of aptamer technique shed bright lights on the practical detection of mycotoxins since aptamers exhibit distinct advantages such as simple and inexpensive synthesis, good stability, resistance to harsh conditions, easy modification, and convenient regeneration. , At present, various aptasensors have been reported to detect mycotoxins based on electrochemistry, fluorescence, and chemiluminesence . However, aptamer-based sensors always require chemical labeling, which brings about complexity and high cost and limit its practical use.…”

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confidence: 99%

Label-Free Quantifications of Multiplexed Mycotoxins by G-Quadruplex Based on Photonic Barcodes

et al. 2020

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Multiplexed quantification of mycotoxins is of great significance in food safety. Here, novel photonic crystal (PhC) barcodes with G-quadruplex aptamer encapsulated for label-free multiplex mycotoxins quantification are developed. The probes are immobilized on PhC barcodes to form a molecular beacon (MB), which contains the sequences of mycotoxin aptamers and a Gquadruplex. In the presence of the target, the hairpin structure of MB would open and the region of the G-quadruplex is exposed, which subsequently combines with Thioflavin T (ThT) to produce fluorescence. The relative fluorescence intensity increased as the mycotoxins concentration increased in a linear range from 1.0 pg/ mL to 100 ng/mL. Moreover, the multiplexed mycotoxins quantification could be achieved by tuning the structural color of the PhC barcodes. We demonstrate that this method with high accuracy and specificity for multiplexed detection of mycotoxins, with the sensitivity of the detection as low as 0.70 pg/mL. Our results show that G-quadruplex-encapsulated PhC barcodes offer a novel simple and label-free pathway toward the multiplex screen assay of mycotoxins for food safety.

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Aptamer Conformation-Cooperated Enzyme-Assisted Surface-Enhanced Raman Scattering Enabling Ultrasensitive Detection of Cell Surface Protein Biomarkers in Blood Samples

Cited by 23 publications

References 50 publications

Nucleic Acids Analysis

Nucleic Acids Analysis

The Development of Electrochemical Aptasensor Based on DNA Aptamers Modified by Redox Markers for Detection of Leukemia Jurkat Cells

Label-Free Quantifications of Multiplexed Mycotoxins by G-Quadruplex Based on Photonic Barcodes

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