Aptamer‐Functionalized Barcodes in Herringbone Microfluidics for Multiple Detection of Exosomes

Abstract

Tumor‐derived exosomes are vital for clinical dynamic and accurate tumor diagnosis, thus developing sensitive and multiple exosomes detection technology has attracted remarkable attention of scientists. Here, a novel herringbone microfluidic device with aptamer‐functionalized barcodes integration for specific capture and multiple detection of tumor‐derived exosomes is presented. The barcodes with core–shell constructions are obtained by partially replicating the periodically ordered hexagonal close‐packaged colloidal crystal beads. As their inverse opal hydrogel shell possesses rich interconnected pores, the barcodes could provide abundant surface area for functionalization of DNA aptamers to realize specific recognition of target exosomes. Besides, the encoded structure colors of the barcodes can be maintained stably during the detection events as their hardish cores are with sufficient mechanical strength. It is demonstrated that by embedding these barcodes in herringbone groove microfluidic device with designed patterns, the specific capture efficiency and synergetic detection of multiple tumor‐derived exosomes in peripheral blood can be significantly improved due to enhanced resistance of turbulent flow. These features make the aptamer‐functionalized barcodes and herringbone microfluidics integrated platform promising for exosomes extraction and dynamic tumor diagnosis.

Magnetic photonic crystals for biomedical applications

“…The top-down strategy mainly relies on traditional microfabrication techniques such as lithography, etching, chemical evaporation, and deposition, which directly manufacture micro/nanostructures with the aid of high-precision computer-assisted instruments. [38][39][40][41][42][43][44][45][46] However, such a manner has been restricted due to the high cost, complex operations, and limited nanoscale precision. In contract, the bottom-up strategy, mainly referred as self-assembly, could achieve synthesis of hierarchical highly-ordered PhC architectures (one-, two-, and three-dimension) with low cost, high efficiency, and delicate nanoscales, as schemed in Figure 2A.…”

Wrinkled Interfaces: Taking Advantage of Anisotropic Wrinkling to Periodically Pattern Polymer Surfaces

“…Periodically patterned polymer film surfaces have been used as functional building blocks in many devices yet challenges remain in their fabrication during the last decades evidenced by the numerous applications generated from them. [ 1 , 2 , 3 , 4 ] Periodic patterning of polymer films serves great functional purposes for applications ranging from optical elements, [ 5 , 6 ] microfluidic devices, [ 7 , 8 ] flexible and stretchable electronics [ 9 , 10 ] as well as templates for constructing complex hierarchical structures [ 11 , 12 ] just to mention few of them.…”

Hydrogel Encapsulated Core–Shell Photonic Barcodes for Multiplex Biomarker Quantification

“…The most common and versatile approaches are the planar array and suspension arrays. 14,15 Compared with a planar array, a suspension array has been widely used because of its high flexibility, rapid reaction speed, and outstanding detection repeatability. 16,17 Suspension arrays usually depend on different coding techniques, including optical, graphic, magnetic, and shape methods.…”

“…Multiplex assays have been applied to detect and quantify various analytes in drug discovery, clinical diagnosis, gene expression, and so on. The most common and versatile approaches are the planar array and suspension arrays. , Compared with a planar array, a suspension array has been widely used because of its high flexibility, rapid reaction speed, and outstanding detection repeatability. , Suspension arrays usually depend on different coding techniques, including optical, graphic, magnetic, and shape methods. In optical coding, photonic crystal barcodes have attracted more and more attention in high-throughput multiregeneration detection .…”