Aptamer-functionalized microtubules for continuous and selective concentration of target analytes

Kim, Minseok; Kim, Taesung

doi:10.1016/j.snb.2014.06.070

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…Ionic hydrogel has extensively utilized as a perm-selective material for ICP operation in various literature. [33][34][35][36][37][38][39][40] Using this system, the capillary force (or capillarity) of the perm-selective nanoporous hydrogel instead of an electric bias drove the fluid into the hydrogel matrix, while its co-ions in the fluid were rejected to enter the hydrogel. This new kind of ICP was named as Capillarity ICP (CICP).…”

Section: Introductionmentioning

confidence: 99%

Capillarity ion concentration polarization for spontaneous biomolecular preconcentration mechanism

Lee

Son

et al. 2016

Biomicrofluidics

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Ionic hydrogel-based ion concentration polarization devices have been demonstrated as platforms to study nanoscale ion transport and to develop engineering applications, such as protein preconcentration and ionic diodes/transistors. Using a microfluidic system composed of a perm-selective hydrogel, we demonstrated a micro/nanofluidic device for the preconcentration of biological samples using a new class of ion concentration polarization mechanism called "capillarity ion concentration polarization" (CICP). Instead of an external electrical voltage source, the capillary force of the perm-selective hydrogel spontaneously generated an ion depletion zone in a microfluidic channel by selectively absorbing counter-ions in a sample solution. We demonstrated a reasonable preconcentration factor ($100-fold/min) using the CICP device. Although the efficiency was lower than that of conventional electrokinetic ICP operation due to the absence of a drift ion migration, this mechanism was free from the undesirable instability caused by a local amplified electric field inside the ion depletion zone so that the mechanism should be suitable especially for an application where the contents were electrically sensitive. Therefore, this simple system would provide a point-of-care diagnostic device for which the sample volume is limited and a simplified sample handling is demanded. V C 2016 AIP Publishing LLC. [http://dx

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Section: Introductionmentioning

confidence: 99%

Capillarity ion concentration polarization for spontaneous biomolecular preconcentration mechanism

Lee

Son

et al. 2016

Biomicrofluidics

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“…3d , implying that gating worked reliably under these conditions. This result will aid the design and fabrication of a small molecule pre-concentrator on a chip for portable biosensors and biochips 29 , 30 .…”

Section: Resultsmentioning

confidence: 97%

Evaporation-driven transport-control of small molecules along nanoslits

Seo

Kim

2021

Nat Commun

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Understanding and controlling the transport mechanisms of small molecules at the micro/nanoscales is vital because they provide a working principle for a variety of practical micro/nanofluidic applications. However, most precedent mechanisms still have remaining obstacles such as complicated fabrication processes, limitations of materials, and undesired damage on samples. Herein, we present the evaporation-driven transport-control of small molecules in gas-permeable and low-aspect ratio nanoslits, wherein both the diffusive and advective mass transports of solutes are affected by solvent evaporation through the nanoslit walls. The effect of the evaporation flux on the mass transport of small molecules in various nanoslit-integrated micro/nanofluidic devices is characterized, and dynamic transport along the nanoslit is investigated by conducting numerical simulations using the advection-diffusion equation. We further demonstrate that evaporation-driven, nanoslit-based transport-control can be easily applied to a micro/nanofluidic channel network in an independent and addressable array, offering a unique working principle for micro/nanofluidic applications and components such as molecule-valves, -concentrators, -pumps, and -filters.

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Section: Passive Separation Group 1: Hydrodynamics-based Separationmentioning

confidence: 99%

“…Microfluidic sieving is a method of separating particles according to their characteristic size by applying a flow to an integrated porous partition (e.g., membrane, hydrogel, or nanoparticle clusters), and the behaviors are dependent according to filter characteristics (e.g., pore size, pore density, and stiffness) and the direction and type of applied forces [ 58 , 59 , 60 , 61 , 62 , 63 ]. As shown in Figure 1 A, Kim et al [ 62 ] fabricated multilayered microchannel networks to integrate nanoporous hydrogels (~100 nm pore size) within microfluidic channel networks as mechanical sieving structures. As shown in Figure 2 A(i), in the sample channel, the red-fluorescent target protein bound to a molecular carrier (microtubule) with a length of microscale was unable to pass through the hydrogel structure, while red-fluorescent non-target biomolecules freely penetrated the membrane, resulting in continuous and selective separation of target proteins from protein mixtures.…”

Section: Passive Separation Group 1: Hydrodynamics-based Separationmentioning

confidence: 99%

“…( ii ) Non-target proteins with green fluorescence ( ii ) penetrated the hydrogel membrane, ( iii ) while red-fluorescent target biomolecules bound to long-molecular carriers (microtubules, >10 μm in length) were selectively filtered by the nanoporous membrane. Reprinted with permission from Elsevier B.V. [ 62 ]; ( B ) ( i ) Separation principle of deterministic lateral displacement (DLD)-based blood cell separation. ( ii ) Experimental results of blood separation using DLD device.…”

Section: Figurementioning

confidence: 99%

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Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

Choe

Kim

2021

Biosensors

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Separation of micro- and nano-sized biological particles, such as cells, proteins, and nucleotides, is at the heart of most biochemical sensing/analysis, including in vitro biosensing, diagnostics, drug development, proteomics, and genomics. However, most of the conventional particle separation techniques are based on membrane filtration techniques, whose efficiency is limited by membrane characteristics, such as pore size, porosity, surface charge density, or biocompatibility, which results in a reduction in the separation efficiency of bioparticles of various sizes and types. In addition, since other conventional separation methods, such as centrifugation, chromatography, and precipitation, are difficult to perform in a continuous manner, requiring multiple preparation steps with a relatively large minimum sample volume is necessary for stable bioprocessing. Recently, microfluidic engineering enables more efficient separation in a continuous flow with rapid processing of small volumes of rare biological samples, such as DNA, proteins, viruses, exosomes, and even cells. In this paper, we present a comprehensive review of the recent advances in microfluidic separation of micro-/nano-sized bioparticles by summarizing the physical principles behind the separation system and practical examples of biomedical applications.

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Aptamer-functionalized microtubules for continuous and selective concentration of target analytes

Cited by 9 publications

References 31 publications

Capillarity ion concentration polarization for spontaneous biomolecular preconcentration mechanism

Capillarity ion concentration polarization for spontaneous biomolecular preconcentration mechanism

Evaporation-driven transport-control of small molecules along nanoslits

Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

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