Aptamer-mediated colorimetric and electrochemical detection of Pseudomonas aeruginosa utilizing peroxidase-mimic activity of gold NanoZyme

Das, Ritu; Dhiman, Abhijeet; Kapil, Arti; Bansal, Vipul; Sharma, Tarun

doi:10.1007/s00216-018-1555-z

Cited by 197 publications

(93 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Besides the above tagging strategies, nanozyme tags are also often used in other immunoassays or immuno‐like assays (Das, Dhiman, Kapil, Bansal, & Sharma, ; Sharma et al., ). For example, Wu, Duan, Qiu, Li, and Wang () reported a colorimetric aptasensor platform for detecting Salmonella enterica serovar typhimurium ( S. typhimurium ) using the POD‐like ZnFe 2 O 4 ‐reduced graphene oxide nanostructures (ZnFe 2 O 4 /rGO) as a signal tag.…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

146

View full text Add to dashboard Cite

The public concerns about agrifood safety call for innovative and reformative analytical techniques to meet the inspection requirements of high sensitivity, specificity, and reproducibility. Enzyme‐mimetic nanomaterials or nanozymes, which combine enzyme‐like properties with nanoscale features, emerge as an excellent tool for quality and safety detection in the agrifood sector, due to not only their robust capacity in detection but also their attraction in future‐oriented exploitations. However, in‐depth understanding about the fundamental principles of nanozymes for food quality and safety detection remains limited, which makes their applications largely empirical. This review provides a comprehensive overview of the principles, designs, and applications of nanozyme‐based detection technique in the agrifood industry. The discussion mainly involves three mimicking types, that is, peroxidase, oxidase, and catalase‐like nanozymes, capable of detecting major agrifood analytes. The current principles and strategies are classified and then discussed in details through discriminating the roles of nanozymes in diverse detection platforms. Thereafter, recent applications of nanozymes in detecting various endogenous ingredients and exogenous contaminants in foods are reviewed, and the outlook of profound developments are explained. Evidenced by the increasing publications, nanozyme‐based detection techniques are narrowing the gap to practical‐oriented food analytical methods, while some challenges in optimization of nanozymes, diversification of recognition‐to‐signal manners, and sustainability of methodology need to conquer in the future.

show abstract

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

146

View full text Add to dashboard Cite

show abstract

“…With great advantages of tunability in activities, high stability as well as easy storage, noble-metal nanozymes show great potential in biosensing. [48][49][50][51][52][53][54][55][56][57][58][59][60] Numerous noble metal nanomaterials such as AuNPs, [48][49][50][51] Pt NPs, [52,53] PdPt, [54] PtAu, [55,56] PdCu, [57] FeCu NPs, [58] Pd@Pt NPs [59] and Au@Pt core/shell nanorods, [46,60] etc. have been widely studied for the detection of microbial pathogens.…”

Section: Detection Of Pathogenic Microorganisms By Nanozymes 21 Nobmentioning

confidence: 99%

“…AuNPs are the most widely studied noble metal-based nanozymes due to their excellent enzymatic activities, good biocompatibility and the availability of versatile bioconjugation means. [22,[48][49][50][51] Recently, Ahmed et al proposed a dual enhanced visual immunosensor integrated with AuNPs for the detection of avian influenza A virus (IAV) (H5N1). 3,3',5,5'-tetramethylbenzidine (TMB) first catalyzed the reduction of an antibodygold ion mixture to blue colored AuNPs.…”

Section: Mono-metal-based Nanozymesmentioning

confidence: 99%

“…Recently, another two studies for AuNP-mediated bacteria detection using aptamers or antibodies were conducted. Das and co-workers [50] exploited peroxidase-like AuNPs in combination with specific aptamers for the colorimetric and electrochemical detection of Pseudomonas aeruginosa. Due to the electrochemical activity of TMB, the nanozyme-based colorimetric method could be transformed into an electrochemical assay, achieving the dual mode detection of P. aeruginosa.…”

Section: Mono-metal-based Nanozymesmentioning

confidence: 99%

See 1 more Smart Citation

Recent Progress of Nanozymes in the Detection of Pathogenic Microorganisms

et al. 2020

View full text Add to dashboard Cite

Infectious diseases are among the world's principal health problems. It is crucial to develop rapid, accurate and cost‐effective methods for the detection of pathogenic microorganisms. Recently, considerable progress has been achieved in the field of inorganic enzyme mimics (nanozymes). Compared with natural enzymes, nanozymes have higher stability and lower cost. More interestingly, their properties can be designed for various demands. Herein, we introduce the latest research progress on the detection of pathogenic microorganisms by using various nanozymes. We also discuss the current challenges of nanozymes in biosensing and provide some strategies to overcome these barriers.

show abstract

“…[1][2][3] Until now, a variety of nanomaterials have been used to mimic the activity of naturally occurring enzymes, [4][5][6][7][8] such as nanocarbon materials, [9][10][11] fullerene, 12 carbon nitride, 13 Fe 3 O 4 nanoparticles, 14 mesoporous silica nanoparticles, 15 dopamine nanoparticles, 16 melanin nanoparticles, 17 and metal-organic complexes. 18 Noble metalbased nanomaterials have also shown signicant advantages as enzyme mimetics; 19,20 gold nanoparticles (AuNPs) possess excellent stability, good biocompatibility, ease of functionalization, and unique optical, and surface properties. [21][22][23][24] The tailored surface functionalization of gold nanoparticles controls the interface between AuNPs and biomolecules, [25][26][27] and it is crucial for development and design of tunable catalytic activities.…”

Section: Introductionmentioning

confidence: 99%

A novel nanozyme based on selenopeptide-modified gold nanoparticles with a tunable glutathione peroxidase activity

et al. 2020

View full text Add to dashboard Cite

show abstract

Aptamer-mediated colorimetric and electrochemical detection of Pseudomonas aeruginosa utilizing peroxidase-mimic activity of gold NanoZyme

Cited by 197 publications

References 50 publications

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Recent Progress of Nanozymes in the Detection of Pathogenic Microorganisms

A novel nanozyme based on selenopeptide-modified gold nanoparticles with a tunable glutathione peroxidase activity

Contact Info

Product

Resources

About