Aptamers directly radiolabeled with technetium-99m as a potential agent capable of identifying carcinoembryonic antigen (CEA) in tumor cells T84

Correa, Cristiane Rodrigues; Barros, André Luís Branco de; Ferreira, Carolina de Aguiar; Góes, Alfredo M.; Cardoso, Valbert Nascimento; Andrade, Antero Silva Ribeiro de

doi:10.1016/j.bmcl.2014.02.048

Cited by 35 publications

(14 citation statements)

References 17 publications

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“…Recently, a new method was developed for radiolabelling aptamers with 99m Tc where the two aptamers -Apt3 and Apt3-amine -selected against the well characterized human tumor associated antigen CEA were used. In binding assay the radiolabeled aptamer showed high specificity and affinity to T84 cells making the aptamer a promising agent for the identification of CEA present in tumor cells (Correa et al 2014). One more method was developed for the detection of CEA by capillary electrophoresis (CE) which is based on the combination of graphene oxide (GO) and quantum dots labeled with aptamer (QD-aptamer).…”

Section: Burkitt Lymphoma Detectionmentioning

confidence: 99%

Application of aptamers in diagnostics, drug-delivery and imaging

et al. 2016

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Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/ toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.

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Section: Burkitt Lymphoma Detectionmentioning

confidence: 99%

Application of aptamers in diagnostics, drug-delivery and imaging

et al. 2016

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“…In 2014, for the first time, the direct 99m Tc-labeling of aptamers was reported by Correa C.R. et al [23]. Two aptamers, with and without primary amine group, were selected and radiolabeled with 99m TcO 4 − in the presence of stannous chloride as a reducing agent.…”

Section: Radiolabeling Of Ligands For Spect Imagingmentioning

confidence: 99%

“…The aforementioned aptamers were directly labeled with 99m Tc by addition of an aliquot of Na 99m TcO 4 in the sealed vial, containing Ethylenediamine- N , N ′-diacetic acid (EDDA) and tricine. Both aptamers’ radiolabeling showed high radiochemical efficiencies and considerable in vitro stability [23]. The next direct radiolabeling of aptamers was performed on several unmodified aptamers with an amine-ended linker by Cao et al in 2015, and on (1→3)-β-D-glucan aptamers by de Sousa Lacerda et al in 2017 [24,25].…”

Section: Radiolabeling Of Ligands For Spect Imagingmentioning

confidence: 99%

Radiolabeling of Nucleic Acid Aptamers for Highly Sensitive Disease-Specific Molecular Imaging

2018

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Aptamers are short single-stranded DNA or RNA oligonucleotide ligand molecules with a unique three-dimensional shape, capable of binding to a defined molecular target with high affinity and specificity. Since their discovery, aptamers have been developed for various applications, including molecular imaging, particularly nuclear imaging that holds the highest potential for the clinical translation of aptamer-based molecular imaging probes. Their easy laboratory production without any batch-to-batch variations, their high stability, their small size with no immunogenicity and toxicity, and their flexibility to incorporate various functionalities without compromising the target binding affinity and specificity make aptamers an attractive class of targeted-imaging agents. Aptamer technology has been utilized in nuclear medicine imaging techniques, such as single photon emission computed tomography (SPECT) and positron emission tomography (PET), as highly sensitive and accurate biomedical imaging modalities towards clinical diagnostic applications. However, for aptamer-targeted PET and SPECT imaging, conjugation of appropriate radionuclides to aptamers is crucial. This review summarizes various strategies to link the radionuclides to chemically modified aptamers to accomplish aptamer-targeted PET and SPECT imaging.

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“…Labeling with 99m Tc was performed by the direct method according to Correa et al (2014) [10]. For the labeling reaction 111.6 mol of tricine and 28.3 mol of EDDA were added to 300 µL of 0.9% saline.…”

Section: Aptamer Radiolabeling With 99m Tc and Radiolabel Yield Determinationmentioning

confidence: 99%

Peptidoglycan aptamers biodistribution in infection-bearing mice

Ferreira¹,

Lacerda²,

Santos³

et al. 2018

Nucl Med Biomed Imaging

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Introduction: Acid Nucleic aptamers are short single-stranded oligonucleotides that display high affinity and selectivity for a given target. Aptamers contain many features that are advantageous for radiopharmaceuticals development. Peptidoglycan is a cell wall polymer common to both Gram-positive and Gram-negative bacteria. In the present study, the potential of two peptidoglycan aptamers for bacterial infection foci identification was evaluated.Material and methods: The peptidoglycan aptamers were labeled with 99m Tc by the direct method and the stability of each 99m Tc-aptamer complex was evaluated in saline, plasma and in presence of cysteine. The aptamers degradation by plasma nucleases was also assessed. Bacterial-infected (Staphylococcus aureus) mice and fungalinfected mice (Candida albicans) were used for the ex vivo biodistribution studies with the 99m Tc-aptamers.Results and discussion: The aptamers were not degraded by plasma nucleases. High radiolabel yields were obtained by the direct method and the complexes were stable in presence of saline and plasma. Some trans chelation was observed in the presence of cysteine. The 99m Tc-pepdigoglycan aptamers uptake in the bacterial infection foci were significantly higher than the control (a radiolabeled oligonucleotide library) and their uptake in the fungal infection model. Conclusion:Both radiolabeled peptidoglycan aptamers present specific uptake in the bacterial infection foci highlighting the potential of these molecules as radiotracer for bacterial infection.

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Aptamers directly radiolabeled with technetium-99m as a potential agent capable of identifying carcinoembryonic antigen (CEA) in tumor cells T84

Cited by 35 publications

References 17 publications

Application of aptamers in diagnostics, drug-delivery and imaging

Application of aptamers in diagnostics, drug-delivery and imaging

Radiolabeling of Nucleic Acid Aptamers for Highly Sensitive Disease-Specific Molecular Imaging

Peptidoglycan aptamers biodistribution in infection-bearing mice

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