AQP1 gene expression is upregulated by arginine vasopressin and cyclic AMP agonists in trophoblast cells

Belkacemi, Louiza; Beall, Marie H.; Magee, Thomas R.; Pourtemour, Margaret; Ross, Michael G.

doi:10.1016/j.lfs.2008.04.014

Cited by 32 publications

(25 citation statements)

References 58 publications

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“…In line with the lung Aqp1 up-regulation reported here, is well known that AP1, SP1 and CREB are selectively activated in pulmonary hypoxia [17] and could be part of the hypoxic response of AQP1. Furthermore, CREB activation by cAMP and binding of this to HBS [47], [48] in the Aqp1 promoter may constitute an alternative explanation for transcriptional regulation of AQP1 by cAMP [49], [50].…”

Section: Resultsmentioning

confidence: 99%

Functional and Transcriptional Induction of Aquaporin-1 Gene by Hypoxia; Analysis of Promoter and Role of Hif-1α

et al. 2011

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Aquaporin-1 (AQP1) is a water channel that is highly expressed in tissues with rapid O2 transport. It has been reported that this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show that hypoxia increases Aqp1 mRNA and protein levels in tissues, namely mouse brain and lung, and in cultured cells, the 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase in the water permeability of 9L cells exposed to hypoxia, supporting the view that hypoxic Aqp1 up-regulation has a functional role. To investigate the molecular mechanisms underlying this regulatory process, transcriptional regulation was studied by transient transfections of mouse endothelial cells with a 1297 bp 5′ proximal Aqp1 promoter-luciferase construct. Incubation in hypoxia produced a dose- and time-dependent induction of luciferase activity that was also obtained after treatments with hypoxia mimetics (DMOG and CoCl2) and by overexpressing stabilized mutated forms of HIF-1α. Single mutations or full deletions of the three putative HIF binding domains present in the Aqp1 promoter partially reduced its responsiveness to hypoxia, and transfection with Hif-1α siRNA decreased the in vitro hypoxia induction of Aqp1 mRNA and protein levels. Our results indicate that HIF-1α participates in the hypoxic induction of AQP1. However, we also demonstrate that the activation of Aqp1 promoter by hypoxia is complex and multifactorial and suggest that besides HIF-1α other transcription factors might contribute to this regulatory process. These data provide a conceptual framework to support future research on the involvement of AQP1 in a range of pathophysiological conditions, including edema, tumor growth, and respiratory diseases.

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Section: Resultsmentioning

confidence: 99%

Functional and Transcriptional Induction of Aquaporin-1 Gene by Hypoxia; Analysis of Promoter and Role of Hif-1α

et al. 2011

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“…The luminal epithelium of the pig uterus is responsible for endometrial remodeling for non-invasive implantation as well as expresses specific factors that prevent invasion of porcine embryo (Wollenhaupt et al 2011). Hence, the integrity of both the maternal and fetal tissues is not disrupted by the blood vessels that develop from the blastocyst and those from the uterus (Carson et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Expression of Aquaporin 1 and 5 and Their Regulation by Ovarian Hormones, Arachidonic Acid, Forskolin and cAMP During Implantation in Pigs

Skowrońska

Młotkowska²,

Majewski³

et al. 2016

Physiol Res

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Aquaporin proteins (AQPs) are a family of channels expressed in numerous mammalian tissues, where they play a fundamental role in regulating water transport across cell membranes. Based on reports that AQPs are present in the reproductive system and participate in reproductive processes, our aim was to investigate the effect of progesterone (P4), estradiol (E2), oxytocin (OT), arachidonic acid (AA), forskolin (FSK) and cyclic adenosine monophosphate (cAMP) on AQP1 and AQP5 expression at mRNA and protein levels in porcine uterine explants from Days 14-16 of gestation in order to determine if they play a role in implantation period in pigs. Quantitative real time PCR and Western-blot analysis revealed that the uterine explants treated with FSK and cAMP produce delayed, but long-term effects on AQP1 abundance (24 h) while AQP5 had a rapid and sustained response to FSK and cAMP in protein content (3 and 24 h). AA increases gene and protein content of AQP1 after longer exposition whereas AQP5 increases after 3 h only at the protein level. Both AQPs potentially remains under control of steroid hormones. OT has been shown to increase AQP1, and decrease AQP5 mRNA, without visible changes in protein content. P4, E2, AA, FSK and cAMP caused the appearance of AQP5 expression in the basolateral plasma membrane of the epithelial cells. The staining represents most likely AQP5 functioning mechanism for both absorption and reabsorption across the glandular epithelium.

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“…The RNA concentration was determined by spectrophotometry at an absorbance of 260 nm, and integrity was assessed by agarose gel electrophoresis and ethidium bromide staining. Reverse transcription was performed as described previously (Belkacemi et al, 2008). Briefly, in a 20 μl reaction 2μg total RNA was reverse transcribed using the Qiagen Omniscript kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Real time polymerase chain reaction (PCR) was carried out as described previously (Belkacemi et al, 2008) using the Sequence Detection System 7000 (Applied Biosystems, Foster City, CA). Briefly, real time PCR was performed using the qPCR Master Mix Plus for SYBR green I (Eurogentec, San Diego, CA).…”

Section: Methodsmentioning

confidence: 99%

Central insulin sensitivity in male and female juvenile rats

Keen-Rhinehart

Desai

Ross

2009

Hormones and Behavior

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The incidence of juvenile obesity is increasing at an alarming rate. In adults, central insulin administration decreases hypothalamic orexigenic neuropeptides, food intake and body weight more effectively in males than females. Mechanisms regulating energy balance in juvenile animals are inherently different from those in adults due to differences in growth rates and hormonal milieu. Therefore, we sought to determine if central insulin treatment in juvenile rats (4 wk) would have similar sex-dependent effects on food intake as those reported in adult rats. Twenty four hour food intake was measured following icv saline or insulin (0.01 or 0.1 U) prior to the onset of dark phase of the light cycle. An additional set of animals was used to assess the effects of central insulin on hypothalamic orexigenic (NPY, AgRP) and anorexigenic (POMC) neuropeptide mRNA expression. In both males and females, insulin reduced meal size initially (first 4h) and later decreased meal frequency (4-24 h) to reduce cumulative food intake. Consistent with this, central insulin decreased hypothalamic NPY and AgRP and increased POMC mRNA expression. In contrast to adult studies, there were no demonstrated sex differences. These studies indicate that juvenile females and males are equally sensitive to central insulin anorexigenic effects, perhaps due to a lack of circulating gonadal hormones. The anorexigenic responsiveness of both genders suggests a potential pharmacologic approach to childhood obesity.

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AQP1 gene expression is upregulated by arginine vasopressin and cyclic AMP agonists in trophoblast cells

Cited by 32 publications

References 58 publications

Functional and Transcriptional Induction of Aquaporin-1 Gene by Hypoxia; Analysis of Promoter and Role of Hif-1α

Functional and Transcriptional Induction of Aquaporin-1 Gene by Hypoxia; Analysis of Promoter and Role of Hif-1α

Expression of Aquaporin 1 and 5 and Their Regulation by Ovarian Hormones, Arachidonic Acid, Forskolin and cAMP During Implantation in Pigs

Central insulin sensitivity in male and female juvenile rats

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