AQUA_2020_1080: Sperm management of European catfish (Silurus glanis L.) for effective reproduction and genetic conservation

Linhart, Otomar; Cheng, Yu; Rodina, Marek; Gela, David; Tučková, Vladimíra; Shelton, William L.; Tinkir, Merve; Memiş, Devrim; Xin, Miaomiao

doi:10.1016/j.aquaculture.2020.735620

Cited by 11 publications

(13 citation statements)

References 47 publications

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“…On the other hand, Hagendorn and Carter [1] did not state the level of hatching. Although in other fish species, such as European catfish, it was found to be better (increased sperm motility, VCL, and VSL when activated and fertilized with hatching level) to store milt for 1 day after stripping or 5 h before freezing in an immobilization solution [20,21,58]. In our study, zebrafish sperm aging is much faster than that in larger fresh-and warm-water fish species such as European catfish and common carp [59].…”

Section: Fertilization and Swim-up Larvae Ratescontrasting

confidence: 46%

“…Spermatozoa of freshwater fishes contaminated with urine showed a similar course of activation during stripping, leading to full arrest if an extender is not used to control the surrounding osmolarity so as to prevent motility. This category of fishes includes zebrafish [18,25,33,34] and the results of the present paper, European catfish (Silurus glanis) [21,35], tench [36,37], asp [16], tilapia (Oreochromis mossambicus) [38], and marine fishes such as Senegalese sole (Solea senegalensis) [39] and turbot (Scophthalmus maximus) [40]. In the case of zebrafish, this problem is even more critical because of the very small size of the fish, which makes it difficult to empty the urine from the bladder prior to milt collection (see Video S1).…”

Section: Sperm Motilitymentioning

confidence: 74%

“…Therefore, a higher incidence of milt contamination is possible in these small-bodied species. To prevent spermatozoa spontaneous movement due to low osmolarity of the urine or excrement, which results in a rapid decrease in fertility, it is important to collect milt in a suitable extender (immobilizing solution), which inhibits sperm movement and preserves its fertilizing ability [17,18,20,21]. After milt collection, the zebrafish laboratory guides recommend time limits to use gametes efficiently.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Sperm Management and Fertilization in Zebrafish (Danio rerio (Hamilton))

Cheng

Franěk

Rodina

et al. 2021

Animals

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The aim of the present study was to investigate the spontaneous motility of spermatozoa and to optimize sperm collection, short-term sperm storage, and fertilization in zebrafish Danio rerio. The movement of spermatozoon in water was propagated along the flagellum at 16 s after sperm activation then damped from the end of the flagellum for 35 s and fully disappeared at 61 s after activation. For artificial fertilization, milt must be added to an immobilizing solution, which stops the movement of sperm and keeps the sperm motionless until fertilization. E400 and Kurokura as isotonic solutions were shown to be suitable extenders to store sperm for fertilization for 6 h. E400 stored sperm for 12 h at 0–2 °C. Sperm motility decreased only to 36% at 12 h post stripping for the E400 extender and to 19% for the Kurokura extender. To achieve an optimal level of fertilization and swim-up larvae rates, a test tube with a well-defined amount of 6,000,000 spermatozoa in E400 extender per 100 eggs and 100 µL of activation solution has proven to be more successful than using a Petri dish. The highest fertilization and swim-up larvae rates reached 80% and 40–60%, respectively, with milt stored for 1.5 h in the E400 extender at 0–2 °C.

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Section: Fertilization and Swim-up Larvae Ratescontrasting

confidence: 46%

Section: Sperm Motilitymentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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Optimization of Sperm Management and Fertilization in Zebrafish (Danio rerio (Hamilton))

Cheng

Franěk

Rodina

et al. 2021

Animals

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“…However, studies focused on sperm cell aging in vivo have been largely limited in shes, reviewed by Kowalski and Cejko (2019). Sperm short-term storage in vitro undergoes aging pressure as in vivo storage (Saad et al 1988;Linhart et al 2020a). In some sh species such as European cat sh (Silurus glanis), good quality sperm is better preserved in vitro than in vivo (Linhart et al 2004).…”

Section: Introductionmentioning

confidence: 99%

In vivo and in vitro aging of common carp Cyprinus carpio sperm after multiple hormonal application and stripping of males

Zhang

Cheng

Linhartová

et al. 2022

Fish Physiol Biochem

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The present study on common carp (Cyprinus carpio L.) sperm was designed to evaluate changes in sperm phenotypic variables during multiple sperm stripping with sperm storage: a) in vivo and b) in vitro. Similar males were multiple injected with carp pituitary (CP) 3 times 3 days apart. Sperm were stored in vivo in the body cavity for 0.5 days (fresh sperm) and 3 days (old sperm) after CP application, then sperm were collected and diluted with a carp extender 1:1 and stored in vitro on ice for 0, 3 and 6 days. In general, fresh sperm from the rst stripping had slightly better quality and quantity than old sperm from the second and third stripping, especially in the phenotypic parameters of number of total spermatozoa and number of total motile spermatozoa (P < 0.05). The highest kinetic and quantitative sperm variables were obtained in fresh and old sperm just after sperm collection at 0 days and then they decreased during in vitro sperm storage up to 6 days (P < 0.05). The fertilization, hatching and malformation rates from fresh sperm were similar compared with the old sperm. From the rst stripping, fresh sperm, even stored for 6 days in vitro, showed fertility, hatching and malformations at 92.5%, 91.5% and 1.3%, respectively. Multiple hormonal treatments with multiple male stripping together with 0.5 days of in vivo sperm storage followed by 6 days of in vitro storage are methods that can be recommended for use in common carp aquaculture.

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“…Several strategies were developed to improve sperm quality. A more critical procedure involves euthanasia and subsequent testicular mincing in saline solution, as performed in species that present urine contamination, such as small fish species (Kopeika et al, 2003) and catfish (Linhart et al, 2020). Catheterization of the testicular lumen is also a strategy used to obtain sperm without contamination (Sarosiek et al, 2016), but such a procedure is not viable in some species due to fish size or morphology of the urogenital papilla.…”

Section: Introductionmentioning

confidence: 99%

Acute exposure to hyperosmotic conditions reduces sperm activation by urine in the yellowtail tetra Astyanax altiparanae, a freshwater teleost fish

Rocha

Schade

Alves

et al. 2020

Braz. J. Vet. Res. Anim. Sci.

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In freshwater fish with external fertilization, sperm sampling can be contaminated with urine, which triggers motility and gives rise to decreased fertilization success. The maintenance of freshwater fish in hyperosmotic conditions may reduce urine production and improve sperm quality. Thus, the aim of this work was to verify if acute exposure to various NaCl concentrations improves sperm quality in the yellowtail tetra Astyanax altiparanae. Spermiation was induced using a single dose of carp pituitary gland (5 mg kg-1) and the males were maintained at various NaCl concentrations: NaCl 0.00% (control), NaCl 0.45% (hypoosmotic), NaCl 0.9% (isosmotic) and NaCl 1.0% (hyperosmotic) for 6 h at 26 °C. Sperm was collected and verified for activation by urine and motility traits. At 0.00%, 0.45%, and 0.90%, the sperm was motile just after sampling, indicating activation by urine. Surprisingly, at hyperosmotic conditions, no activation was observed. Other sperm and motility parameters did not show any statistical differences, including sperm viability (P = 0.7083), concentration (P = 0.9030), total motility (P = 0.6149), VCL (curvilinear velocity; P = 0.1216), VAP (average path velocity; P = 0.1231) and VSL (straight-line velocity; P = 0.1340). Our results indicate that acute maintenance at hyperosmotic conditions eliminates sperm activation by urine and maintains sperm quality. Such a new procedure is interesting for both basic and applied sciences, including reproductive practice in fish.

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AQUA_2020_1080: Sperm management of European catfish (Silurus glanis L.) for effective reproduction and genetic conservation

Cited by 11 publications

References 47 publications

Optimization of Sperm Management and Fertilization in Zebrafish (Danio rerio (Hamilton))

Optimization of Sperm Management and Fertilization in Zebrafish (Danio rerio (Hamilton))

In vivo and in vitro aging of common carp Cyprinus carpio sperm after multiple hormonal application and stripping of males

Acute exposure to hyperosmotic conditions reduces sperm activation by urine in the yellowtail tetra Astyanax altiparanae, a freshwater teleost fish

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