Aqueous extract of Cordyceps sinensis potentiates the antitumor effect of DDP and attenuates therapy-associated toxicity in non-small cell lung cancer via IκBα/NFκB and AKT/MMP2/MMP9 pathways

Huo, Xiaowei; Liu, Chenqi; Bai, Xuelian; Li, Wenjia; Li, Jing; Hu, Xuefeng; Cao, Li

doi:10.1039/c7ra04716a

Cited by 12 publications

(5 citation statements)

References 41 publications

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“…The contents of four nucleosides in FCP were determined by HPLC to control the quality of FCP. The results showed that the composition of uridine, inosine, guanosine, and adenosine in FCP used in this experiment was 0.0833%, 0.0014%, 0.1148%, and 0.1025% (Cai et al, 2018; Huo et al, 2017). We found that the adenosine content in the FCP we used was much higher than the 0.010% stipulated in the 2020 edition of the Chinese Pharmacopoeia (Figure 1 and Table 1).…”

Section: Resultsmentioning

confidence: 67%

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Fermented Cordyceps Powder Attenuates Inflammation and Pulmonary Arterioles Remodeling by Inhibiting p38 MAPK/NF-κB Signaling Pathway in Hypoxic Pulmonary Hypertension Rats

Li,

Chen,

Yang

et al. 2023

Pharmacognosy Magazine

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Background Pulmonary hypertension (PH) is a pulmonary vascular disease caused by a variety of causes and has a poor prognosis. Hypoxic pulmonary hypertension (HPH) is one of the types of PH. Cordyceps sinensis could treat a variety of lung diseases; its pharmacological active ingredients mainly include cordycepin, nucleosides, cordyceps polysaccharides, sterols, and amino acids. Fermented cordyceps powder (FCP) is an alternative to C. sinensis. In order to investigate the effect of FCP on HPH in rats, we established the HPH rat model. Materials and Methods Forty-two male Sprague–Dawley (SD) rats were randomly divided into a control group, hypoxia group, hypoxia + FCP (0.1 g.kg−1.d−1), hypoxia + FCP (0.2 g.kg−1.d−1), hypoxia + FCP (0.4 g.kg−1.d−1), and hypoxia + sildenafil (30 mg.kg−1.d−1) group. Except for the control group, the other five groups of rats were fed in the hypobaric chamber. The corresponding dose was given by intragastric administration, once a day, for 28 days. The mean pulmonary arterial pressure (mPAP) and right ventricle hypertrophy index (RVHI) were measured; hematological parameters white blood cells (WBC), neutrophils (Neu), Lymphocytes (Lym), monocytes (Mon), red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), and platelets (PLT) levels were detected; serum levels of interleukin-1β (IL-1β) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA); the morphological changes of pulmonary arterioles were observed by hematoxylin and eosin (H&E) staining, and the vascular remodeling indexes, vessel wall thickness as a percentage of vascular outer diameter (WT%), vascular wall area as a percentage of total vessel area (WA%), and lumen area as a percentage of the total vascular area (LA%) were calculated; the protein expression levels of IL-1β, IL-6, p-p38, p38, p-IκBα, IκBα, p-p65, and p65 were determined by western blotting. Results FCP could significantly downregulate mPAP, improve pulmonary arteriole remodeling, and reduce the serum levels of IL-1β and IL-6 in HPH rats. In addition, FCP downregulated the protein levels of IL-1β, IL-6, p-p38, p-IκBα, and p-p65. Conclusion FCP could alleviate mPAP and vascular remodeling in HPH rats. Its mechanism could be through inhibiting the p38 mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-κB) signaling pathway and by downregulating the expression of inflammatory factors. Our study might provide a research basis for the therapeutic potential of FCP in HPH.

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Section: Resultsmentioning

confidence: 67%

“…Nucleosides are commonly used as quality control indicators for FCP (Huo et al, 2017). The contents of four nucleosides in FCP were determined by HPLC to control the quality of FCP.…”

Section: Identification Of Nucleoside Components Of Fcpmentioning

confidence: 99%

Fermented Cordyceps Powder Attenuates Inflammation and Pulmonary Arterioles Remodeling by Inhibiting p38 MAPK/NF-κB Signaling Pathway in Hypoxic Pulmonary Hypertension Rats

Li,

Chen,

Yang

et al. 2023

Pharmacognosy Magazine

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“…In, the study also investigated the effects of the species on the development of the H-1299 lung carcinoma cell line, and the results showed that H. erinaceus inhibited the development of this cell line by 72%, P. ostreatus by 61% and G. lucidum by 24% (Sharif et al, 2018). Huo et al (2017) used 12 different cancer cell lines (SK-MEL-28, CoLo205, Raji , HCT-116, Lewis, Hep3B2.1-7,MDAMB-453, K562, HeLa, B16F10, NCI-H460 and A549) to investigate the dose-dependent anticancer effects of two polysaccharides (AECS1 and AECS2) derived from water extracts of the Cordyceps sinensis (AECS) type fungus. As a result of the study, they reported that IC50 values of AECS1 ranged between 0.02-3.2 mg/mL and AECS2 ranged between 0.31-4.08 mg/mL.…”

Section: Anticancer Studies Of Mushroom Extractsmentioning

confidence: 99%

Secondary Metabolites in Fungi

et al. 2022

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Fungi are a biological family that includes multicellular and unicellular eukaryotic organisms in general. They are quite numerous and diverse. It is the second largest group of organisms on Earth after insects. Higher fungi (terrestrial fungi) currently constitute a large and efficiently untapped resource of new pharmacological crops with nutritional values are potentially powerful. Antioxidants prevent cellular damage caused by free radicals in living beings. It has been found that 36% of endophytic mushroom extracts with a high phenolic content show strong antioxidant effects. In one study, they reported that the antioxidant activities of the strains Aspergillus sp., A. niger, A. peyronelii and Chaetomium sp. ranged from 50% to 80% and that this antioxidant activity was due to their high phenolic content. In addition to the antioxidant properties of fungi, it has been shown as a result of research that they show antibacterial activity. Macrofungal β-glucans (β-1,6 glycosidic bonds and mainly β-1,3), which are known to have immunomodulatory and antitumor effects in cancer prevention, can be used in treatment strategies. At the same time, it has been shown in studies that these metabolites obtained from fungi cause fewer side effects when used in treatments, unlike chemical drugs. As a result of phytochemical research, the presence of flavonoids, anthraquinones, steroids, terpenoids, coumarins, phenols and tannins in fungal extracts in general was detected, while alkaloids and saponins were not found. It has been determined that the studies on mushrooms have gained versatile uses since they reveal secondary metabolite components with different biological activities in the structure of these group members.

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“…Nakamura et al [17] found that the growth of mouse Lewis lung cancer cells and B16-BL6 melanoma cells was significantly inhibited because of the adenine nucleoside A3 receptor in tumor cells stimulated by the cordycepin. Huo et al [18] found that cordycepin significantly inhibited the growth of human cervical cancer HeLa cells, human nasopharyngeal carcinoma KB cells and Ehrlich ascites tumor. The survival time of mice inoculated with Ehrlich ascites tumor was only 19 days, while that of mice treated with cordycepin can be extended to 60 days.…”

Section: Fig1 Two Dimensional Structure and Three Dimensional Conformentioning

confidence: 99%

Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell

Cheng

2020

E3S Web Conf.

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Cordycepin, the main active component of Cordyceps militaris, possesses a variety of biological activities. This paper investigated the inhibition of cordycepin on liver tumors. HepG2 cells were treated with cordycepin. The inhibition rates of cell proliferation was detected by MTT, LC3 protein was detected by immunofluorescence method, autophagy was observed by transmission electron microscopy, autophagy related protein was detected by Western blot. The results showed that cordycepin significantly inhibited the proliferation of HepG2 cells, and significantly increased the expression level of LC3 protein (<0.05). and the autophagy increased in the cells. The Atg-related protein analysis showed that cordycepin significantly reduced the p-mTOR / β-actin ratio and p62 / β-actin ratio (<0.01), and significantly increased the LC3II/LC3I ratio (P<0.01). The results suggested that cordycepin inhibited the proliferation by activating autophagy in HepG2 cells.

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Aqueous extract of Cordyceps sinensis potentiates the antitumor effect of DDP and attenuates therapy-associated toxicity in non-small cell lung cancer via IκBα/NFκB and AKT/MMP2/MMP9 pathways

Abstract: Cordyceps sinensisis reported as an invigorant with pleiotropic biological effects that has potential for tumor therapy.

Cited by 12 publications

References 41 publications

Fermented Cordyceps Powder Attenuates Inflammation and Pulmonary Arterioles Remodeling by Inhibiting p38 MAPK/NF-κB Signaling Pathway in Hypoxic Pulmonary Hypertension Rats

Fermented Cordyceps Powder Attenuates Inflammation and Pulmonary Arterioles Remodeling by Inhibiting p38 MAPK/NF-κB Signaling Pathway in Hypoxic Pulmonary Hypertension Rats

Secondary Metabolites in Fungi

Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell

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