Aqueous extract of Tetrapleura tetraptera fruit peels influence copulatory behavior and maintain testicular integrity in sexually mature male Sprague-Dawley rats: Pro-fertility evaluation and histomorphometry evidence

Abstract

Tetrapleura tetraptera (TT) has been used as a spice, dietary supplement and medicine for various ailments. This study evaluate influence of Tetrapleura tetraptera extract on testis and copulatory behavior in sexually mature male rats. Thirty-two male and sixty-four virgin female rats weighing 150–200 g were used for this study. Male rats randomly divided into four groups of eight (n = 8) rats each. Group A: Control given 2 ml distilled water, group B, C and D received 50, 300 and 700 mg/kg bwt TT for 56 days through oral gavage. The female rats were used for fertility test. Testicular histology, histomorphology, copulatory behavior, sperm parameters, testosterone (TET), luteinizing hormone (LH), follicle stimulating hormone (FSH), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and fertility test were investigated. Tetrapleura tetraptera significantly increase sperm count, motility, normal morphology, daily sperm production, efficiency of sperm production, sperm (average path velocity, straight line velocity and curvilinear velocity), TET, LH, FHS, SOD, GPx, CAT, number of pregnant females, number of fetuses, seminiferous diameter, epithelium thickness and decrease abnormal morphology, seminiferous height, tubule lumen and MDA across the group as compared with control group. Improved testicular histological integrity, sexual behaviour and libido by increased frequencies of mount, intromission, ejaculation and ejaculatory latency. Latencies of mount, intromission and post-ejaculation were significantly reduced. Also, observed increase spermatocytes and spermatids showed no significant difference in spermatogonia cell counts. Tetrapleura tetraptera therefore, enhance steroidogenesis, spermatogenesis, and improved testicular histological integrity and boost sexual competence in male rats.

Evaluation of the Phytochemical Composition of Some Commonly Sold Male Herbal Fertility Supplements in Port Harcourt, Rivers State, Nigeria

“…Also Tetrapleura tetraptera (aidan fruit), another component in mascum herbal pride was reported to contain tannins, flavonoid, saponin, triterpenoid and coumarin thereby serving as anti-inflammatory, anti-coagulant and antioxidant agents in a study by Adusei et al [19]. Adelakun et al [20] in his study reveals that this plant improves spermatogenic activity and maintains testicular integrity. Phytochemicals such as saponins, flavonoid, tannins, phenols, alkaloids, terpenes and protodioscin were identified in mascum herbal pride so the observed phytochemicals may be responsible for the increase testicular function attributable to this supplement as seen in a study by Okam et al [21] upon sub-chronic administration of Mascum herbal pride on male albino rats for 90 days.…”

Anti-inflammation Study of Cellulose-Chitosan Biocomposite-Based Tetrapleura tetraptera (Taub) Dried Fruits Aqueous Extract

“…Among the rich Cameroonian Flora, Tetrapleura tetraptera (Taub) holds an important place. Tetrapleura tetraptera (family Fabaceae) is a regular tree distributed throughout tropical Africa, particularly in West, Central, and East African rain forests [6][7][8][9][10][11]. The plant is named Tetrapleura tetraptera (Schum et Thonn) Taubert (Fabaceae-Mimosoideae).…”

“…6 The fourwinged fruit (see Fig. 1) with a fragrant, typically pungent aromatic scent, is used in folkloric medicine for the treatment of various ailments and as spice in the preparation of varieties of soups by E ks and Ibibios of the Niger Delta region of Nigeria, in North-West, South-West and West region of Cameroun in yellow sausage or Nkui and Littoral region of Cameroon for Mbongo sausage [6][7][8][9][10][11]. Tetrapleura tetraptera has been used as medicine for various ailments including in ammatory diseases.…”

“…10 Fruit decoctions and infusions are traditionally used for the management and control of wounds, diabetes mellitus, asthma, hypertension, convulsions, leprosy, mental illness, in ammation, arthritis and rheumatoid pain, control of schistosomiasis, as a general tonic and as a avouring / spice [6,12]. The plant is one of the potential hepatotoxic plants used in Nigeria, it enhances steroidogenesis, spermatogenesis, and improved testicular histological integrity and boosts sexual competence in male rats [8,10]. The aqueous fruit extract possesses analgesic and anticonvulsant properties [12].…”