Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells

Kim, Hye Jin; Kim, Jin Chul; Min, Jung Sun; Kim, Mi-Jee; Kim, Ji Ae; Kor, Myung Ho; Yoo, Hwa Seung; Ahn, Jeong Keun

doi:10.1016/j.jep.2011.04.060

Cited by 65 publications

(46 citation statements)

References 21 publications

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“…The change of cell cycle is found to be essential for restraining the proliferation and reducing the invasion of both human and mouse cancer cells in the development of tumor (38)(39)(40)(41)(42). We also demonstrated similar results of IL-12 in JEG-3 cells in this study.…”

Section: Discussionsupporting

confidence: 83%

Interleukin-12 inhibits cell invasion in choriocarcinoma

Zhang

Shi

et al. 2012

Int J Mol Med

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Abstract. Gestational trophoblastic disease (GTD) is a unique disease that arises from allografting of the conceptus, and has a characteristic morphology and biological behavior. It encompasses a spectrum of interrelated diseases, including hydatidiform mole, invasive mole, choriocarcinoma and placental-site trophoblastic tumor, but its pathogenesis remains unrevealed. Particularly, choriocarcinoma is a highly malignant tumor with poor prognosis. In this study, we cultured the human choriocarcinoma cell line JEG-3 in vitro. After treating the cells with different doses of interleukin (IL)-12, the cell invasion was observed. We also detected the expression of matrix metallopromatrix metalloproteinases (MMP)-9 and tissue inhibitors of metalloproteinases (TIMP)-1 and the cell cycle of JEG-3 cells. Our data indicated that IL-12 inhibits cell invasion in a dose-and time-dependent manner through regulating the expression of MMP-9 and TIMP-1. In addition, treatment with IL-12 redistributes the phases of the cell cycle in JEG-3 cells. These findings suggest an antitumor role of IL-12 in choriocarcinoma, with far reaching possibilities for understanding the mechanisms of IL-12.

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Section: Discussionsupporting

confidence: 83%

Interleukin-12 inhibits cell invasion in choriocarcinoma

Zhang

Shi

et al. 2012

Int J Mol Med

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“…Furthermore, to identify which components of the extract induce apoptosis, all of them should firstly be isolated individually by GC-MS or preparative HPLC and then their effects on the cells should be studied. Kim et al reported that T. terrestris extract could trigger apoptosis in liver cancer cell by preventing the NF-κB factor signaling (8). This finding confirms their observation, however we cannot conclude that inhibition of NF-κB signaling is the sole mechanism involved in apoptosis in our study.…”

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confidence: 84%

Cytotoxic, Anti-Proliferative and Apoptotic Effects of Tribulus terrestris L. Fruit Extract on Human Prostate Cancer Lncap and Colon Cancer HT-29 Cell Lines

Pourali

Yaghoobi

Sormaghi

2016

Jundishapur J Nat Pharm Prod

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“…Trouillas et al (2005) reported that the saponins, structurally similar to diosgenin, present in T. terrestris extracts, might block the cell cycle, suppress proliferation, and induce apoptosis in human sarcoma cell lines. Kim et al (2011) reported that the water extracts of T. terrestris blocked proliferation and induced apoptosis in human liver cancer cells and could be used as an anticancer drug for hepatocellular carcinoma patients.…”

Section: Resultsmentioning

confidence: 99%

Investigation on the toxic potential ofTribulus terrestris in vitro

Asadi

Jannuzzi

Özhan

et al. 2014

Pharmaceutical Biology

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Context: Tribulus terrestris L. (Zygophyllaceae) has been commonly used to energize, vitalize, and improve sexual function and physical performance in men.Objective: This study investigates the potential cytotoxic and genotoxic, and endocrine disrupting activities of T. terrestris in vitro. Materials and methods: The whole T. terrestris plant was extracted with water, methanol, and chloroform. The genotoxic potential of T. terrestris extracts at 3-2400 mg/mL was assessed by Comet assay in a rat kidney cell line (NRK-52E) and by Ames assay in Salmonella typhimurium TA98 and TA100 strains. Endocrine disrupting effects of the extracts at concentrations of 0.22-25 000 mg/mL were assessed by YES/YAS assay in Saccharomyces cerevisiae. Cytotoxic activity of the extracts was determined by the MTT test in NRK-52E cells. The different exposure times were used for four tests (3-48 h). Results: The methanol extract of T. terrestris IC 50 value was 160 mg/mL. The other extracts did not show cytotoxic effects. In the Comet and Ames genotoxicity assays, none of the extracts possessed genotoxic activities at concentrations of 0-2400 mg/mL. Only the water extract of T. terrestris induced frame shift mutations after metabolic activation. The water extract also showed estrogenic activity by YES/YAS assay in S. cerevisiae at concentrations !27 mg/mL (!2.6-fold), while the other T. terrestris extracts had anti-estrogenic properties. Conclusion: Tribulus terrestris had estrogenic and genotoxic activities. The study was useful in determining its toxicological effects and the precautions regarding consumption.

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Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells

Cited by 65 publications

References 21 publications

Interleukin-12 inhibits cell invasion in choriocarcinoma

Interleukin-12 inhibits cell invasion in choriocarcinoma

Cytotoxic, Anti-Proliferative and Apoptotic Effects of Tribulus terrestris L. Fruit Extract on Human Prostate Cancer Lncap and Colon Cancer HT-29 Cell Lines

Investigation on the toxic potential ofTribulus terrestris in vitro

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