Aqueous leaf extract of Passiflora alata Curtis promotes antioxidant and anti-inflammatory effects and consequently preservation of NOD mice beta cells (non-obese diabetic)

Figueiredo, Daniella de; Colomeu, Talita Cristina; Schumacher, Nayara Simon Gonzalez; Stivanin-Silva, Luiz Guilherme; Cazarin, Cínthia Baú Betim; Meletti, Laura Maria Molina; Fernandes, Luís Gustavo Romani; Prado, Marcelo Alexandre; Zollner, Ricardo de Lima

doi:10.1016/j.intimp.2016.03.031

Cited by 23 publications

(22 citation statements)

References 34 publications

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“…rough our results, it might be suggested that the improvement of pancreas function upon HC administration is partly due to the anti-inflammatory and antioxidant properties of HC. Several anti-inflammatory compounds were thus proposed as therapeutic agents for treating diabetes, since they have the potential ability to preserve beta-cell function [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Effects of Fermented Houttuynia cordata Thunb. on Diabetic Rats Induced by a High-Fat Diet with Streptozotocin and on Insulin Resistance in 3T3-L1 Adipocytes

Sakuludomkan

Yeewa

Subhawa

et al. 2021

Journal of Nutrition and Metabolism

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Houttuynia cordata Thunb. (plukaow in Thai language) exhibits several biological properties, and many products of H. cordata are therefore commercially available for human consumption, such as fermented juice or tablets as food supplements. This study aimed to investigate the antidiabetic effects of fermented H. cordata (HC) in high-fat diets and streptozotocin-induced diabetic rats. Oral administration of HC at a dose of 100 mg/kg.bw not only maintained bodyweight, food intake, and water consumption but also reduced blood glucose levels and improved glucose tolerance ability in the diabetic rats. Moreover, HC also decreased oxidative stress markers in serum and inflammatory-related mediators in pancreas tissues, indicating the improvement of pancreatic beta-cell function in the diabetic rats. In order to clarify the mechanism of HC, the effects of ethanolic extract of HC (HCE) on insulin resistance were determined in 3T3-L1 adipocytes. FHE could recover glucose uptake and decrease lipolysis in palmitate-treated 3T3-L1 adipocytes. Taken together, these results demonstrate that HC can improve diabetic symptoms by enhancing insulin sensitivity, reducing oxidative stress, and suppressing inflammation.

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Section: Discussionmentioning

confidence: 99%

Effects of Fermented Houttuynia cordata Thunb. on Diabetic Rats Induced by a High-Fat Diet with Streptozotocin and on Insulin Resistance in 3T3-L1 Adipocytes

Sakuludomkan

Yeewa

Subhawa

et al. 2021

Journal of Nutrition and Metabolism

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“…It has been reported that the leaf extract also has anti-diabetic property which is attributed to the presence of phenolic compounds such as catechin, epicatechin and rutin. These bioactive compounds are known to decrease oxidative stress, apoptosis and the number of inflammatory cells in pancreas [4][5]. P. alata has been used as a sedative and for anxiolytic purposes.…”

Section: Leavesmentioning

confidence: 99%

The active compounds of Passiflora spp and their potential medicinal uses from both in vitro and in vivo evidences

Smruthi

Divya

Archana

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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“…These compounds are associated with beneficial effects in controlling diseases initiated by oxidative stress by their antioxidant properties. The major flavonoid compounds of P. alata that present antioxidative activity include isoorientin, isovitexin, vitexin [ 9 ], rutin, catechin, epicatechin [ 10 ], apigenin and orientin [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, NOD mice treated with aqueous leaf extract of P. alata presented a decrease in proinflammatory infiltrative CD4 + and CD8 + lymphocytes (insulitis) and oxidative stress in the pancreatic islets [ 9 ], contributing to the survival of insulin-producing beta cells in the pancreas islets [ 10 ]. This study aimed to contribute knowledge of phenolic compounds’ in vivo anti-inflammatory effects via in vitro analyses.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and Pro-Oxidant Effect of Polyphenols in Aqueous Leaf Extract of Passiflora alata Curtis on Activated T Lymphocytes from Non-Obese Diabetic (NOD SHILT/J) Mice

et al. 2022

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The antioxidant, anti-inflammatory and antiproliferative properties of Passiflora alata Curtis are due to the presence of polyphenols in its composition. Our previous work showed that non-obese diabetic (NOD) mice undergoing treatment with aqueous leaf extract of P. alata present reduced insulitis in the pancreas, possibly due to its anti-inflammatory properties. However, depending on the concentration and their ability to interact with other molecules, these phenolic compounds may promote oxidation reactions in some cellular components, such as proteins and lipids, thus presenting a pro-oxidant effect. The present work aimed to evaluate the in vitro effects of aqueous leaf extract of P. alata and its polyphenols (vitexin, isoorientin, rutin and catechin) on lymphocyte proliferation and viability, the cell cycle and oxidative stress. Our results showed that T lymphocytes stimulated with concanavalin A mitogen (ConA) and in the presence of IC50 concentrations of P. alata extract and polyphenols undergo cell injury via inhibition of proliferation, with these effects being more pronounced concerning CD4+ T cells (P. alata, 3.54 ± 0.34%; isoorientin, 57.07 ± 6.4%; vitexin, 16.95 ± 1.11%; catechin, 37.9 ± 4.2% and rutin, 40.14 ± 4.5%), compared to the non-treated group (77.17 ± 6.29) (p < 0.0001 for all comparisons). This process includes late apoptosis/necrosis induction (P. alata, 77.5 ± 0.7%; vitexin, 83 ± 3.3%; isoorientin, 83.8 ± 1.4%; catechin, 83 ± 1.9% and rutin, 74.9 ± 3.2, while the control presented 53.6% ± 3.1 (p < 0.0001 for all comparisons)) and mitochondrial depolarization leading to cell-death induction. Furthermore, an in vitro model of a mixed culture of NOD mice T cells with a mouse pancreatic beta-cell line (MIN6) showed increased intracellular nitric oxide and lipid peroxidation in NOD T cells submitted to P. alata extract (46.41 ± 3.08) compared to the untreated control group (33.57 ± 1.99, p = 0.01315). These results suggest that aqueous leaf extract of P. alata and the polyphenols in these leaves represent a target for translational research showing the plant's benefits for developing new drugs with immunomodulatory properties against inflammatory diseases such as diabetes mellitus.

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Aqueous leaf extract of Passiflora alata Curtis promotes antioxidant and anti-inflammatory effects and consequently preservation of NOD mice beta cells (non-obese diabetic)

Cited by 23 publications

References 34 publications

Effects of Fermented Houttuynia cordata Thunb. on Diabetic Rats Induced by a High-Fat Diet with Streptozotocin and on Insulin Resistance in 3T3-L1 Adipocytes

Effects of Fermented Houttuynia cordata Thunb. on Diabetic Rats Induced by a High-Fat Diet with Streptozotocin and on Insulin Resistance in 3T3-L1 Adipocytes

The active compounds of Passiflora spp and their potential medicinal uses from both in vitro and in vivo evidences

Antiproliferative and Pro-Oxidant Effect of Polyphenols in Aqueous Leaf Extract of Passiflora alata Curtis on Activated T Lymphocytes from Non-Obese Diabetic (NOD SHILT/J) Mice

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