Aqueous-Methanol Extract of Gracilaria verrucosa Induces Cytochrome c Release From Mitochondria

Fitria, Mona; Warganegara, Fida Madayanti

doi:10.1016/j.proche.2015.12.071

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…it will allows ROS to penetrates easily to the cells and it will induce apoptosis. 6 As declared by NCBI, Gracilaria verrucosa did not have any toxic effect, analgesic effect and anti-convulsant effect for the patient. So it is assumed that Gracilaria verrucosa ( Fig.…”

Section: Introductionmentioning

confidence: 78%

“…Beside DNA breakdwon, Gracilaria tenuispitata can also reduce glutathione or GSH which caused ROS to easily produced in the cells. 6,7 Gracilaria verrucosa has a secondary metabolit of saponin, that is a substance which has aglycone hydrofobic structure with addtional part of sugar hyrofilic. This structure make saponin becomes sureface-active amphipathic compounds that has many benefits for its biological activity such as cell hemolytic.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Analysis and Anticancer Activity of Seaweed Gracilaria verrucosa against Colorectal HCT-116 Cells

et al. 2018

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In this research, we develop Indonesia marine resource of seaweed Gracilaria verrucosa as an anti-colorectal cancer agent. Seaweed Gracilaria verrucosa which was collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, that is n-hexane, ethylacetate, chloroform and ethanol. The extracts were analyzed by Phytochemical test and Thin Layer Chromatography (TLC). Subsequently, anticancer activity of n-hexane, ethyl acetate, chloroform and ethanol extracts of Gracilaria verrucosa were evaluated against colorectal HCT-116 cells by MTT cell proliferation assay. Based on Phytochemical analysis, the extracts of Gracilaria verrucosa containing secondary metabolite of saponin, while TLC analysis indicated that the extracts were composed by five chemical compounds. Among four concentrated extracts of Gracilaria verrucosa, ethanolic extract showed the strongest anticancer activity against colorectal HCT-116 cells with IC 50 of 43.9 μg/mL.Ethanolic extract of seaweed Gracilaria verrucosa is potential to be further developed as a promising anti-colorectal cancer agents.

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Section: Introductionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis and Anticancer Activity of Seaweed Gracilaria verrucosa against Colorectal HCT-116 Cells

et al. 2018

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“…Studies have shown that this seaweed has shown promising evidence indicating that extract of G. verrucosa is able to induce apoptosis through mitochondrial pathway by involving cytochrome c release from mitochondria. 6 Moreover, the species G.tenuistipitata is able to have apoptosis-based cytotoxicity against oral cancer cells by induction of ROS, DNA damage, and mitochondrial depolarization against oral squamous carcinoma displayed by their secondary metabolites. Most studies about Gracilaria seaweeds have been focused on its anti-hypercholesterolemic, anti-oxidative, anti-inflammatory, and antimicrobial properties but not its anti-cancer activities.…”

Section: In Vitro Evaluation Of Seaweed Gracilaria Verrucosa For Cytomentioning

confidence: 99%

In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells

Dewi

Arsianti²,

Zagloel

et al. 2018

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Background: Seaweed macroalgae of Gracilaria verrucosa has been known to have a potent anticancer activity, however the cytotoxicity against cervical cancer has not been explored further. Objective: This study aims to utilize Indonesia's marine resource which is focused on seaweed macroalgae G. verrucosa as a future anti-cervical cancer agent. Materials and Method: Seaweed G. verrucosa originated from Labuan Aji beach, Nusa Tenggara Barat, Indonesia, extracted, macerated, and fractionated into four organic solvents of different polarity, consisting of hexane, ethyl acetate, chloroform and ethanol. Then, the macroalgae extracts are diluted into 8 different concentrations. Afterwards, in vitro anticancer activity evaluation of hexane, ethyl acetate, chloroform and ethanol extracts of G. verrucosa against cervical HeLa cells were conducted by MTT cell proliferation assay. Triplo mechanism is also applied in this study to increase the accuracy of the results. The anticancer activity is measured using IC 50 value. Results: The four concentrated extracts G. verrucosa showed cytotoxicity against cervical HeLa cells. The greatest anticancer activity is depicted by hexane extract with an IC 50 of 14.94 µg/mL, followed by chloroform (IC 50 15.74 µg/mL), ethyl acetate (IC 50 16.18 µg/mL), and ethanol (IC 50 19.43 µg/mL). Conclusion: Our results clearly indicate that hexane, ethanol, chloroform, and ethyl acetate extracts of seaweed G. verrucosa can be further developed to be anti-cervical cancer agents, with hexane extract displaying the greatest cytotoxic effect.

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A concise review on the bioactive potential of the genus Gracilaria (Rhodophyta)

Rani,

Saini,

Fartyal

et al. 2024

Nucleus

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Aqueous-Methanol Extract of Gracilaria verrucosa Induces Cytochrome c Release From Mitochondria

Cited by 3 publications

References 16 publications

Phytochemical Analysis and Anticancer Activity of Seaweed Gracilaria verrucosa against Colorectal HCT-116 Cells

Phytochemical Analysis and Anticancer Activity of Seaweed Gracilaria verrucosa against Colorectal HCT-116 Cells

In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells

A concise review on the bioactive potential of the genus Gracilaria (Rhodophyta)

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