Aqueous two-phase extraction of phenolic compounds from Sedum dendroideum with antioxidant activity and anti-proliferative properties against breast cancer cells

Enriquez-Ochoa, Daniela; Sánchez‐Trasviña, Calef; Hernández-Sedas, Betsabé; Mayolo‐Deloisa, Karla; Zavala, Judith; Rito‐Palomares, Marco; Valdez-García, Jorge E.

doi:10.1016/j.seppur.2020.117341

Cited by 24 publications

(9 citation statements)

References 40 publications

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“…ATPS, given their aqueous environment and mild conditions, have been largely employed for the recovery and separation of a broad range of biomacromolecules, including proteins, enzymes, antibodies, peptides, and genetic material (Asenjo and Andrews 2011 ; Azevedo et al 2009 ; Sánchez-Trasviña et al 2019 ), as well as low molecular weight compounds with biological activity (Enriquez-Ochoa et al 2020 ; Ghaffari et al 2019 ; Simental-Martínez et al 2014 ). Nevertheless, the application of ATPS for the separation of polysaccharides has been limited, mainly oriented as an extraction technique for polysaccharides from plant sources using ethanol-salt ATPS (Chen et al 2016 ; Cheng et al 2017 ; Wu et al 2017 ; Zhang et al 2018 ; Zhu et al 2020 ), whereas the implementation of ATPS in the downstream processing of polysaccharides from microbial fermentations is practically unexplored.…”

Section: Introductionmentioning

confidence: 99%

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for the industrial production of HA. However, due to its final applications, microbial HA must undergo critical and long purification processes to ensure clinical and cosmetic grade purity. Aqueous two-phase systems (ATPS) have proven to be an efficient technique for the primary recovery of high-value biomolecules. Nevertheless, their implementation in HA downstream processing has been practically unexplored. In this work, polyethylene glycol (PEG)–citrate ATPS were used for the first time for the primary recovery of HA produced with an engineered strain of Streptococcus equi subsp. zooepidemicus. The effects of PEG molecular weight (MW), tie-line length (TLL), volume ratio (VR), and sample load on HA recovery and purity were studied with a clarified fermentation broth as feed material. HA was recovered in the salt-rich bottom phase, and its recovery increased when a PEG MW of 8000 g mol−1 was used. Lower VR values (0.38) favoured HA recovery, whereas purity was enhanced by a high VR (3.50). Meanwhile, sample load had a negative impact on both recovery and purity. The ATPS with the best performance was PEG 8000 g mol−1, TLL 43% (w/w), and VR 3.50, showing 79.4% HA recovery and 74.5% purity. This study demonstrated for the first time the potential of PEG–citrate ATPS as an effective primary recovery strategy for the downstream process of microbial HA.

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Section: Introductionmentioning

confidence: 99%

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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“…Using ATPS, high extraction rate and product purity can be achieved, whilst maintaining the biological activity of extracted compounds. Compared with conventional LLE method, ATPS has certain advantages of mild extraction conditions, less required volume, various self-adjusting factors, high extraction rate, short phase separation time, easy amplification and operation, as well as suitability for industrial application ( Enriquez-Ochoa, et al, 2020 ).…”

Section: Methods For Honey Authenticity Verificationmentioning

confidence: 99%

A comprehensive review of the current trends and recent advancements on the authenticity of honey

Zhang,

Gu,

Liu

et al. 2023

Food Chemistry: X

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“…A single-factor test was conducted to investigate the effects of ammonium sulfate concentration (12%, 14%, 16%, 18%, and 20%), ethanol concentration (35%, 40%, 45%, 50%, and 55%), liquid-to-material ratio (15,20,25,30, and 35 mL/g), extraction temperature (30,40,50,60, and 70 • C), and extraction time (20,40,60,80, and 100 min) on the yield of CCSPs.…”

Section: Single-factor Testmentioning

confidence: 99%

“…Among them, the alcohol-salt ATPS provides the additional advantage of being relatively cheap and having low viscosity as well as easy solvent recovery. It has been widely used in the extraction of natural products such as polysaccharides [15][16][17][18] and phenolic compounds [19][20][21]. To the best of our knowledge, there is no report on the extraction of CCSPs via the ATPE technique.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Two-Phase Extraction, Antioxidant and Renal Protective Effects of Polysaccharides from Spores of Cordyceps cicadae

Zheng

et al. 2022

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The aim of this study was to investigate the aqueous two-phase extraction (ATPE), in vitro antioxidant, and in vivo renal protective effects of polysaccharides from spores of Cordyceps cicadae (CCSPs). The optimal ATPE parameters were as follows: an extraction temperature of 61 °C, an ammonium sulfate concentration of 18%, an ethanol concentration of 40%, a liquid-to-material ratio of 33 mL/g, and an extraction time of 60 min. Under these parameters, the CCSPs yield was 6.96 ± 0.11% (n = 3), which was consistent with the predicted yield (6.92%). Among the three purified polysaccharide fractions, CCSP-2 displayed stronger scavenging activities against DPPH radicals and hydroxyl radicals, reducing power and ferrous-ion-chelating ability to a greater extent than CCSP-1 and CCSP-3. CCSP-2 exhibited its protective effect in lipopolysaccharide (LPS)-induced septic acute kidney injury (AKI) mice by significantly alleviating renal edema; reducing 24 h urine protein, blood urea nitrogen (BUN), and serum creatinine (SCr); inhibiting the release of serum proinflammatory cytokines; boosting the activities of antioxidant enzymes; and reducing the levels of oxidative damage products. These results suggest that CCSP-2 exerted its protective effect against LPS-induced septic AKI in mice through anti-inflammatory and antioxidant pathways.

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Aqueous two-phase extraction of phenolic compounds from Sedum dendroideum with antioxidant activity and anti-proliferative properties against breast cancer cells

Cited by 24 publications

References 40 publications

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

A comprehensive review of the current trends and recent advancements on the authenticity of honey

Aqueous Two-Phase Extraction, Antioxidant and Renal Protective Effects of Polysaccharides from Spores of Cordyceps cicadae

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