Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan

Abstract

A bacterial strain designated antisso-27(T), previously isolated from saltpan in Taiwan while screening for bacteria for algicidal activity, was characterized using the polyphasic taxonomic approach. Strain antisso-27(T) was Gram-negative, aerobic, brownish yellow colored, rod-shaped, non-flagellated and non-gliding. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-27(T) belonged to the genus Aquimarina within the family Flavobacteriaceae with relatively low sequence similarities of 94.0-96.6% to other valid Aquimarina spp. It contained iso-C(17:0) 3-OH, iso-C(15:0), iso-C(16:0), iso-C(15:1) and iso-C(15:0) 3-OH as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone. Major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, an uncharacterized aminolipid and five uncharacterized phospholipids. Strain antisso-27(T) employed direct mode of algicidal lysis to Chlorella vulgaris strain 211-31; nevertheless, it released an algicidal substance against M. aeruginosa strain MTY01. This is the first study that the Aquimarina species possesses both direct and indirect algicidal activities. On the basis of the phylogenetic and phenotypic data, strain antisso-27(T) should be classified as representing a novel species, for which the name A. salinaria sp. nov. is proposed. The type strain is A. salinaria antisso-27(T) (= BCRC 80080(T) = LMG 25375(T)).

Advanced nutrient removal from surface water by a consortium of attached microalgae and bacteria: A review

“…inhibit the quorum sensing of bacteria (Borowitzka, 2016). Similarly, some bacteria can secrete toxic active substances such as Streptomycin, which may affect photosynthesis-related gene transcription and block electron transport of microalgae (Perales-Vela et al, 2016), or excrete metabolites presenting algicidal effects (Chen et al, 2012). A competition of CO 2 between nitrifying bacterial and microalgae could eventually occur under carbon limited conditions (García et al, 2017).…”

Aquimarina hainanensis sp. nov., isolated from diseased Pacific white shrimp Litopenaeus vannamei larvae

“…At the time of writing, 17 species of the genus Aquimarina have been recognized or reclassified from other genera. All were isolated from the marine environment: seven from seawater, Aquimarina muelleri (Nedashkovskaya et al, 2005), A. latercula (Lewin 1969;Nedashkovskaya et al, 2006), A. addita (Yi & Chun, 2011), A. longa (Yu et al, 2013), A. megaterium , A. atlantica (Li et al, 2014a) and A. pacifica ; two from sediment, A. brevivitae (Yoon et al, 2006;Nedashkovskaya et al, 2006) and A. macrocephali (Miyazaki et al, 2010); two from mussels, A. mytili and A. gracilis (Park et al, 2013); two from marine sponges, A. spongiae (Yoon et al, 2011) and A. amphilecti (Kennedy et al, 2014); one from sea urchin, A. intermedia (Nedashkovskaya et al, 2006); one from the surface of the marine red alga Porphyra haitanensis, A. agarilytica (Lin et al, 2012); one from saltpan, A. salinaria (Chen et al, 2012); and one from Pacific white shrimp (Penaeus vannamei), A. penaei (Li et al, 2014b). One novel bacterial strain, designated M124 T , was isolated from diseased Pacific white shrimp Litopenaeus vannamei larvae.…”

Aurantiacicella marina gen. nov., sp. nov., a myxol-producing bacterium from surface seawater

“…Cells are Gram-stain-negative, non-motile, non-gliding, aerobic (Chen et al, 2012;Lewin, 1969;Li et al, 2014;Lin et al, 2012;Miyazaki et al, 2010;Nedashkovskaya et al, 2005Nedashkovskaya et al, , 2006Park et al, 2012Park et al, , 2013Yi & Chun, 2011;Yoon et al, 2006Yoon et al, , 2011Yu et al, 2013Yu et al, , 2014Zhang et al, 2014) Description of Aurantiacicella marina sp. nov.…”

“…S2. The major polar lipids of strain 2A-8 T were phosphatidylethanolamine, an unidentified aminolipid and five unidentified lipids; five more unidentified lipids, two unidentified aminolipids, lysophosphatidylethanolamine (phosphatidylethanolamine where one fatty acid chain is missing from the glycerol backbone) and an Nedashkovskaya et al, 2005); 3, A. addita JC2680 T , A. agarilytica ZC1 T , A. atlantica 22II-S11-z7 T , A. gracilis PSC32 T , A. intermedia KMM 6258 T , A. latercula LMG 1343 T , A. longa SW024 T , A. macrocephali JAMB N27 T , A. megaterium XH134 T , A. mytili PSC33 T , A. pacifica SW150 T , A. salinaria antisso-27 T , A. spongiae A6 T and A. brevivitae SMK-19 T (Chen et al, 2012;Li et al, 2014;Lin et al, 2012;Miyazaki et al, 2010;Nedashkovskaya et al, 2006;Park et al, 2012Park et al, , 2013Yi & Chun, 2011;Yoon et al, 2006Yoon et al, , 2011Yu et al, 2013Yu et al, , 2014Zhang et al, 2014); 4, Dokdonia donghaensis DSW-1 T and DSW-21 ; 5, D. diaphoros MSKK-32 T , D. eikasta PMA-26 T and D. genika Cos-13 T (Khan et al, 2006). Values are percentages of the total fatty acids and are shown when present at $0.5 %.…”