Arabidopsis abi1-1 and abi2-1 Phosphatase Mutations Reduce Abscisic Acid-Induced Cytoplasmic Calcium Rises in Guard Cells

Allen, Grant; Kuchitsu, Kazuyuki; Chu, Sarah P.; Murata, Yoshiyuki; Schroeder, Julian I.

doi:10.2307/3871054

Cited by 133 publications

(218 citation statements)

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“…In microsomal membranes of V. faba guard cells, both H 1 -ATPase properties were also reduced by micromolar concentrations of Ca 21 , the inhibitory effect of Ca 21 being reversed by the addition of the Ca 21 chelator BAPTA (Kinoshita et al, 1995). Allen et al (1999) reported that anion channel activation by ABA could be mimicked by an external application of Ca 21 , indicating that such an activation was Ca 21 -dependent in Arabidopsis guard cells. Here, we confirmed in Arabidopsis cell suspensions this result by an opposite approach.…”

Section: Ca 21 Has a Dual Effect Leading To Plasma Membrane Depolarizmentioning

confidence: 98%

Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca2+ Dependent

et al. 2004

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In Arabidopsis suspension cells a rapid plasma membrane depolarization is triggered by abscisic acid (ABA). Activation of anion channels was shown to be a component leading to this ABA-induced plasma membrane depolarization. Using experiments employing combined voltage clamping, continuous measurement of extracellular pH, we examined whether plasma membrane H 1 -ATPases could also be involved in the depolarization. We found that ABA causes simultaneously cell depolarization and medium alkalinization, the second effect being abolished when ABA is added in the presence of H 1 pump inhibitors. Inhibition of the proton pump by ABA is thus a second component leading to the plasma membrane depolarization. The ABA-induced depolarization is therefore the result of two different processes: activation of anion channels and inhibition of H 1 -ATPases. These two processes are independent because impairing one did not suppress the depolarization. Both processes are however dependent on the [Ca 21 ] cyt increase induced by ABA since increase in [Ca 21 ] cyt enhanced anion channels and impaired H 1 -ATPases.Abscisic acid (ABA) induces the depolarization of the plasma membrane (Thiel et al., 1992). This depolarization has been interpreted as the consequence of the activation of anion channels in stomatal guard cells of Vicia faba (Blatt, 1990;Schroeder and Keller, 1992;Thiel et al., 1992;Ward et al., 1995), Nicotiana benthamiana and Commelina communis (Schwartz et al., 1995;Leonhardt et al., 1999). In addition, we demonstrated that the extracellular perception of ABA in Arabidopsis suspension cells was necessary for the activation of anion channels inducing the plasma membrane depolarization (Ghelis et al., 2000a), and recently we showed that this anion channel stimulation induced by extracellular ABA perception was dependent on phospholipase D activities (Hallouin et al., 2002). In guard cells that are the most studied plant cell model used for the dissection of ABA signaling pathways (Assmann, 1993;Schroeder et al., 2001), two distinct anion channels, rapid anion channels (R-type) and slow anion channels (S-type), were proposed to participate in the plasma membrane depolarization (Schroeder and Keller, 1992;. Both R-type and S-type channels have been suggested to contribute to an initial phase of the depolarization, while maintenance of the depolarized state of the plasma membrane was only attributed to the S-type anion channels (Schroeder and Keller, 1992). The mechanisms by which ABA activates anion channels are not entirely understood . In V. faba guard cells, activation of anion channels by ABA can be observed without variation of the cytoplasm calcium concentration, suggesting that the ABA-induced anion efflux is calcium-independent (Schwarz and Schroeder, 1998). However, numerous data support the calcium dependence of the anion channel activation in response to ABA. Some of the anion channels involved in a long-term plasma membrane depolarization are Ca 21 -sensitive and therefore are activated by an increase in cyto...

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Section: Ca 21 Has a Dual Effect Leading To Plasma Membrane Depolarizmentioning

confidence: 98%

Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca2+ Dependent

et al. 2004

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“…Dominant negative mutations in PP2C-type protein phosphatases abi1 and abi2 disrupt ABA-activation of Ca 2+ channels [17,124]. ROS production was also disrupted in the abi1-1 mutant but not in the abi2-1 mutant, leading to the hypothesis that abi2-1 affects responses downstream of ROS production [17] and abi1-1 affects responses upstream of ROS production (Fig.…”

Section: Ca 2+ Channels: Roles In Guard Cell Signal Transductionmentioning

confidence: 99%

Roles of ion channels and transporters in guard cell signal transduction

2007

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Stomatal complexes consist of pairs of guard cells and the pore they enclose. Reversible changes in guard cell volume alter the aperture of the pore and provide the major regulatory mechanism for control of gas exchange between the plant and the environment. Stomatal movement is facilitated by the activity of ion channels and ion transporters found in the plasma membrane and vacuolar membrane of guard cells. Progress in recent years has elucidated the molecular identities of many guard cell transport proteins, and described their modulation by various cellular signal transduction components during stomatal opening and closure prompted by environmental and endogenous stimuli.

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“…abo6 was also crossed with abi1-1and abi2-1 and the double mutants were identified (Allen et al, 1999).…”

Section: Genetic Mapping and Mutant Complementationmentioning

confidence: 99%

DEXH Box RNA Helicase–Mediated Mitochondrial Reactive Oxygen Species Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and Auxin Signaling

et al. 2012

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It is well known that abscisic acid (ABA) promotes reactive oxygen species (ROS) production through plasma membraneassociated NADPH oxidases during ABA signaling. However, whether ROS from organelles can act as second messengers in ABA signaling is largely unknown. Here, we identified an ABA overly sensitive mutant, abo6, in a genetic screen for ABAmediated inhibition of primary root growth. ABO6 encodes a DEXH box RNA helicase that is involved in regulating the splicing of several genes of complex I in mitochondria. The abo6 mutant accumulated more ROS in mitochondria, as established using a mitochondrial superoxide indicator, circularly permuted yellow fluorescent protein. Two dominant-negative mutations in ABA insensitive1 (abi1-1) and abi2-1 greatly reduced ROS production in mitochondria. The ABA sensitivity of abo6 can also be compromised by the atrbohF mutation. ABA-mediated inhibition of seed germination and primary root growth in abo6 was released by the addition of reduced GSH and exogenous auxin to the medium. Expression of auxin-responsive markers ProDR5:GUS (for synthetic auxin response element D1-4 with site-directed mutants in the 59-end from soybean):bglucuronidase) and Indole-3-acetic acid inducible2:GUS was greatly reduced by the abo6 mutation. Hence, our results provide molecular evidence for the interplay between ABA and auxin through the production of ROS from mitochondria. This interplay regulates primary root growth and seed germination in Arabidopsis thaliana.

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Arabidopsis abi1-1 and abi2-1 Phosphatase Mutations Reduce Abscisic Acid-Induced Cytoplasmic Calcium Rises in Guard Cells

Cited by 133 publications

References 0 publications

Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca2+ Dependent

Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca2+ Dependent

Roles of ion channels and transporters in guard cell signal transduction

DEXH Box RNA Helicase–Mediated Mitochondrial Reactive Oxygen Species Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and Auxin Signaling

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