Arabidopsis ADF5 promotes stomatal closure by regulating actin cytoskeleton remodeling in response to ABA and drought stress

Qian, Dong; Zhang, Zhe; He, Jinbo; Pan, Zuanfeng; Ou, Xiaobin; Li, Tian; Niu, Lipan; Nan, Qiong; Niu, Yue; He, Wenliang; An, Lizhe; Jiang, Kun; Xiang, Yun

doi:10.1093/jxb/ery385

Cited by 82 publications

(69 citation statements)

References 56 publications

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“…Naturally, the eight proteins induced in response to drought in all genotypes (DHAR1, KAT, LTP1, HSP 18.6, GRXC6, RNS3, ADF3, and TIM18) are prime candidates for the selection of drought-tolerance markers. Most of these proteins have been reported to play important roles in response to plant hormones [14][15][16][17] and are involved in detoxification of reactive oxygen species (ROS) [15,18,19]. Protein transport to mitochondria through the membrane is facilitated by TIM complexes, potentially delivering antioxidant enzymes to mitochondria and enabling ROS detoxification during water deficits.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)

Hamzelou

Pascovici

Kamath

et al. 2020

IJMS

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Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance.

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Section: Discussionmentioning

confidence: 99%

“…Its role in the leaves of droughted plants remains unknown. While ADF-3 and other members of the ADF family, such as ADF-5 [16], have a function in abscisic acid-induced stomatal closure and therefore transpiration, it needs to be established that stomatal complexes alone could strongly influence the abundance of this group of proteins.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)

Hamzelou

Pascovici

Kamath

et al. 2020

IJMS

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“…It was shown that Atabf4/areb2 and Atabf3 mutants were more sensitive to drought than WT, whereas overexpression of AtABF2/AREB1 ensured better drought tolerance due to increased expression of LEA genes ( Fujita et al, 2005 ; Yoshida et al, 2010 ). AtABFs/AREBs promote stomatal closure and chlorophyll catabolism ( Gao et al, 2016 ; Qian et al, 2019 ). Importantly, AtABF2/AREB1, AtABF4/AREB2, AtABF3, and AtABF1 show redundancy during regulation of ABA-mediated drought responses.…”

Section: Introductionmentioning

confidence: 99%

Barley ABI5 (Abscisic Acid INSENSITIVE 5) Is Involved in Abscisic Acid-Dependent Drought Response

et al. 2020

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ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABAdependent regulation of barley response to drought stress. We identified barley TILLING mutants carrying different alleles in the HvABI5 gene and we studied in detail the physiological and molecular response to drought and ABA for one of them. The hvabi5.d mutant, carrying G1751A transition, was insensitive to ABA during seed germination, yet it showed the ability to store more water than its parent cv. "Sebastian" (WT) in response to drought stress. The drought-tolerant phenotype of hvabi5.d was associated with better membrane protection, higher flavonoid content, and faster stomatal closure in the mutant under stress compared to the WT. The microarray transcriptome analysis revealed up-regulation of genes associated with cell protection mechanisms in the mutant. Furthermore, HvABI5 target genes: HVA1 and HVA22 showed higher activity after drought, which may imply better adaptation of hvabi5.d to stress. On the other hand, chlorophyll content in hvabi5.d was lower than in WT, which was associated with decreased photosynthesis efficiency observed in the mutant after drought treatment. To verify that HvABI5 acts in the ABA-dependent manner we analyzed expression of selected genes related to ABA pathway in hvabi5.d and its WT parent after drought and ABA treatments. The expression of key genes involved in ABA metabolism and signaling differed in the mutant and the WT under stress. Droughtinduced increase of expression of HvNCED1, HvBG8, HvSnRK2.1, and HvPP2C4 genes was 2-20 times higher in hvabi5.d compared to "Sebastian". We also observed a faster stomatal closure in hvabi5.d and much higher induction of HvNCED1 and HvSnRK2.1 genes after ABA treatment. Together, these findings demonstrate that HvABI5 plays a role in regulation of drought response in barley and suggest that HvABI5 might be engaged in the fine tuning of ABA signaling by a feedback regulation between biosynthetic and signaling events. In addition, they point to different mechanisms of HvABI5 action in regulating drought response and seed germination in barley.

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“…We previously showed that both knockout/knockdown and overexpression of the F-actin reorganization regulators SCAB1 and CKL2 delayed stomatal closure (32,35). A defect in ADF5 also resulted in delayed stomatal closure (51), while overexpressing ADF1 led to partially or entirely closed stomata (52). The morphological changes in the guard cell vacuole are closely related to stomatal movement (53).…”

Section: Discussionmentioning

confidence: 99%

AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis

Zheng

Jiang

Wang

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Stomatal movement is essential for plant growth. This process is precisely regulated by various cellular activities in guard cells. F-actin dynamics and vacuole morphology are both involved in stomatal movement. The sorting of cargoes by clathrin adaptor protein (AP) complexes from the Golgi to the vacuole is critical for establishing a normal vacuole morphology. In this study, we demonstrate that the medium subunit of the AP3 complex (AP3M) binds to and severs actin filaments in vitro and that it participates in the sorting of cargoes (such as the sucrose exporter SUC4) to the tonoplast, and thereby regulates stomatal closure in Arabidopsis thaliana. Defects in AP3 or SUC4 led to more rapid water loss and delayed stomatal closure, as well as hypersensitivity to drought stress. In ap3m mutants, the F-actin status was altered compared to the wild type, and the sorted cargoes failed to localize to the tonoplast. AP3M contains a previously unidentified F-actin binding domain that is conserved in AP3M homologs in both plants and animals. Mutations in the F-actin binding domain of AP3M abolished its F-actin binding activity in vitro, leading to an aberrant vacuole morphology and reduced levels of SUC4 on the tonoplast in guard cells. Our findings indicate that the F-actin binding activity of AP3M is required for the precise localization of AP3-dependent cargoes to the tonoplast and for the regulation of vacuole morphology in guard cells during stomatal closure.

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Arabidopsis ADF5 promotes stomatal closure by regulating actin cytoskeleton remodeling in response to ABA and drought stress

Abstract: ADF5 promotes stomatal closure by regulating actin filament dynamics, and members of the ABF/AREB transcription factor family may serve as potential upstream regulators of ADF5 in the drought stress/ABA signaling pathway.

Cited by 82 publications

References 56 publications

Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)

Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)

Barley ABI5 (Abscisic Acid INSENSITIVE 5) Is Involved in Abscisic Acid-Dependent Drought Response

AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis

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