Arabidopsis cryptochrome 1 controls photomorphogenesis through regulation of H2A.Z deposition

Mao, Zhilei; Wei, Xuxu; Li, Ling; Xu, Peng; Zhang, Jingyi; Wang, Wenxiu; Guo, Tongtong; Kou, Shuang; Wang, Wanting; Miao, Langxi; Cao, Xiaoli; Zhao, Jiachen; Yang, Guangqiong; Zhang, Shilong; Li, Hongli; Hong-yuan, Yang

doi:10.1093/plcell/koab091

Cited by 48 publications

(36 citation statements)

References 89 publications

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“…Given that ARP6 is another core subunit in SWR1 complex that interacts with CRY1 (Mao et al, 2021), we also tested whether phyB would interact with ARP6. We performed in vitro pull‐down assays using MBP‐ARP6 protein as bait.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, the far‐red light photoreceptor phyA was found to interact with SWC6 and ARP6 (Figures 1A, C, E, S2; Mao et al, 2021), and mutation in either SWC6 or ARP6 leads to long hypocotyl in far‐red light (Mao et al, 2021). Previous studies have demonstrated that phyA and phyB share a set of downstream factors to transduce light signals, such as COP1, SPAs, and PIFs (Jang et al, 2010; Lu et al, 2015; Sheerin et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…All Arabidopsis thaliana plants were in the Columbia background. The phyA‐211 phyB‐9 , swc6 (SAIL_536_A05), arp6 (GARLIC_599_G03), and hy5 hyh mutants (Nagatani et al, 1993; Reed et al, 1993; Deal et al, 2005; March‐Diaz et al, 2007; Lian et al, 2018), and SWC6‐Flag‐OX , SWC6‐Flag‐OX / hy5 hyh , Myc‐phyB‐OX , and phyB‐YFP‐OX transgenic lines were described previously (Wang et al, 2010; Xu et al, 2019; Mao et al, 2021). The phyB‐YFP‐OX/arp6 transgenic plant was generated by genetic crossing and identified by DNA genotyping.…”

Section: Methodsmentioning

confidence: 99%

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Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.Z deposition and photomorphogensis in Arabidopsis

Wei

Wang

et al. 2021

JIPB

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Light serves as a crucial environmental cue which modulates plant growth and development, and which is controlled by multiple photoreceptors including the primary red light photoreceptor, phytochrome B (phyB). The signaling mechanism of phyB involves direct interactions with a group of basic helix‐loop‐helix (bHLH) transcription factors, PHYTOCHROME‐INTERACTING FACTORS (PIFs), and the negative regulators of photomorphogenesis, COP1 and SPAs. H2A.Z is an evolutionarily conserved H2A variant which plays essential roles in transcriptional regulation. The replacement of H2A with H2A.Z is catalyzed by the SWR1 complex. Here, we show that the Pfr form of phyB physically interacts with the SWR1 complex subunits SWC6 and ARP6. phyB and ARP6 co‐regulate numerous genes in the same direction, some of which are associated with auxin biosynthesis and response including YUC9, which encodes a rate‐limiting enzyme in the tryptophan‐dependent auxin biosynthesis pathway. Moreover, phyB and HY5/HYH act to inhibit hypocotyl elongation partially through repression of auxin biosynthesis. Based on our findings and previous studies, we propose that phyB promotes H2A.Z deposition at YUC9 to inhibit its expression through direct phyB‐SWC6/ARP6 interactions, leading to repression of auxin biosynthesis, and thus inhibition of hypocotyl elongation in red light.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.Z deposition and photomorphogensis in Arabidopsis

Wei

Wang

et al. 2021

JIPB

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“…However, the molecular mechanism by which light signals trigger the specific interaction of these two proteins remains to be understood. As CRY1 was recently reported to facilitate the formation and recruitment of SWR1c for photomorphogenesis (Mao et al, 2021), the photoreceptors (i.e. PHYs and CRYs) may contribute to mediating the light-dependent interaction between NF-YCs and ARP6, requiring a further investigation.…”

Section: Discussionmentioning

confidence: 99%

NF‐YCs modulate histone variant H2A.Z deposition to regulate photomorphogenic growth in Arabidopsis

Zhang

Qian

Huang

et al. 2021

JIPB

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In plants, light signals trigger a photomorphogenic program involving transcriptome changes, epigenetic regulation, and inhibited hypocotyl elongation. The evolutionarily conserved histone variant H2A.Z, which functions in transcriptional regulation, is deposited in chromatin by the SWI2/SNF2‐RELATED 1 complex (SWR1c). However, the role of H2A.Z in photomorphogenesis and its deposition mechanism remain unclear. Here, we show that in Arabidopsis thaliana, H2A.Z deposition at its target loci is induced by light irradiation via NUCLEAR FACTOR‐Y, subunit C (NF‐YC) proteins, thereby inhibiting photomorphogenic growth. NF‐YCs physically interact with ACTIN‐RELATED PROTEIN6 (ARP6), a key component of the SWR1c that is essential for depositing H2A.Z, in a light‐dependent manner. NF‐YCs and ARP6 function together as negative regulators of hypocotyl growth by depositing H2A.Z at their target genes during photomorphogenesis. Our findings reveal an important role for the histone variant H2A.Z in photomorphogenic growth and provide insights into a novel transcription regulatory node that mediates H2A.Z deposition to control plant growth in response to changing light conditions.

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“…Another contribution demonstrated that CRY1 can physically interact with members of the SWI2/SNF2-Related 1 (SWR1) chromatin remodelling complex responsible for the deposition of the histone variant H2A.Z [ 170 ]. In particular, the authors showed that under blue light conditions CRY1 interacts with SWR1 complex subunit 6 (SWC6) and Actin-related protein 6 (ARP6) thus promoting H2A.Z deposition on HY5 targets and inhibiting hypocotyl elongation in Arabidopsis.…”

Section: Cell Cycle Epigenetics and Circadian Clockmentioning

confidence: 99%

Cryptochromes and the Circadian Clock: The Story of a Very Complex Relationship in a Spinning World

Lopez

Fasano

Perrella

et al. 2021

Genes

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Cryptochromes are flavin-containing blue light photoreceptors, present in most kingdoms, including archaea, bacteria, plants, animals and fungi. They are structurally similar to photolyases, a class of flavoproteins involved in light-dependent repair of UV-damaged DNA. Cryptochromes were first discovered in Arabidopsis thaliana in which they control many light-regulated physiological processes like seed germination, de-etiolation, photoperiodic control of the flowering time, cotyledon opening and expansion, anthocyanin accumulation, chloroplast development and root growth. They also regulate the entrainment of plant circadian clock to the phase of light–dark daily cycles. Here, we review the molecular mechanisms by which plant cryptochromes control the synchronisation of the clock with the environmental light. Furthermore, we summarise the circadian clock-mediated changes in cell cycle regulation and chromatin organisation and, finally, we discuss a putative role for plant cryptochromes in the epigenetic regulation of genes.

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Arabidopsis cryptochrome 1 controls photomorphogenesis through regulation of H2A.Z deposition

Cited by 48 publications

References 89 publications

Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.Z deposition and photomorphogensis in Arabidopsis

Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.Z deposition and photomorphogensis in Arabidopsis

NF‐YCs modulate histone variant H2A.Z deposition to regulate photomorphogenic growth in Arabidopsis

Cryptochromes and the Circadian Clock: The Story of a Very Complex Relationship in a Spinning World

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