Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response

Chen, Huan; Hwang, Jin-Woo; Lim, Chan Ju; Kim, Dool Yi; Lee, Sang Yeol; Lim, Chae Oh

doi:10.1016/j.bbrc.2010.09.038

Cited by 140 publications

(98 citation statements)

References 19 publications

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“…Among others, we observe misregulation of RING MEMBRANE-ANCHOR 1 (RMA1), an uncharacterized protein with possible E3 ubiquitin ligase activity, and DREB2C, a member of the DREB subfamily A-2 of the ERF/ AP2 transcription factor family implicated in drought stress responses (Fig. 4 B-E) (46)(47)(48)(49). Interestingly, in addition to DREB2C, 39 other transcription factors identified as CCA1 targets in this study recently have been shown to interact with the CCA1 promoter in a yeast one-hybrid assay (Dataset S3) (50).…”

Section: Countmentioning

confidence: 99%

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Nagel

Doherty

Pruneda‐Paz

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

234

279

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The circadian clock in Arabidopsis exerts a critical role in timing multiple biological processes and stress responses through the regulation of up to 80% of the transcriptome. As a key component of the clock, the Myb-like transcription factor CIRCADIAN CLOCK ASSOCIATED1 (CCA1) is able to initiate and set the phase of clockcontrolled rhythms and has been shown to regulate gene expression by binding directly to the evening element (EE) motif found in target gene promoters. However, the precise molecular mechanisms underlying clock regulation of the rhythmic transcriptome, specifically how clock components connect to clock output pathways, is poorly understood. In this study, using ChIP followed by deep sequencing of CCA1 in constant light (LL) and diel (LD) conditions, more than 1,000 genomic regions occupied by CCA1 were identified. CCA1 targets are enriched for a myriad of biological processes and stress responses, providing direct links to clock-controlled pathways and suggesting that CCA1 plays an important role in regulating a large subset of the rhythmic transcriptome. Although many of these target genes are evening expressed and contain the EE motif, a significant subset is morning phased and enriched for previously unrecognized motifs associated with CCA1 function. Furthermore, this work revealed several CCA1 targets that do not cycle in either LL or LD conditions. Together, our results emphasize an expanded role for the clock in regulating a diverse category of genes and key pathways in Arabidopsis and provide a comprehensive resource for future functional studies.genome-wide | circadian clock | clock-controlled outputs | transcriptional regulation I n anticipation of the daily changes in light and temperature, the plant's internal circadian clock regulates a large portion of the transcriptome, nearly 80% in rice, poplar, and Arabidopsis (1, 2). The clock provides temporal coordination of multiple biological functions to ensure optimal efficiency. For example, before dawn, photosynthetic transcripts accumulate in anticipation of the expected sunlight. Even when shifted to constant light (LL) and temperature, in the absence of day/night cues, transcripts associated with photosynthesis are still up-regulated before the subjective day (1-4). Not only are light-and energyrelated activities restricted to particular times, but other aspects of plant growth, including water use, hormone activity, UVB response, and low-temperature response, show this gating effect (5-9). This temporal partitioning of biological responses ultimately provides an optimal integration of the plant's organismal functions with the environment.The circadian clock in plants involves a posttranscriptional component and a well-studied transcriptional-translation feedback regulation between the Myb-like transcription factors CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) and a member of the PSEUDO RESPONSE REGULATOR (PRR) family, TIMING OF CAB2 EXPRESSION 1 (TOC1) (10-16). Together these three components contribute t...

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Section: Countmentioning

confidence: 99%

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Nagel

Doherty

Pruneda‐Paz

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

234

279

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“…DREB2 genes were initially identified as drought and high-salinity response genes (Liu et al, 1998), and were later shown to be induced in response to heat stress as well Sakuma et al, 2006). Recent studies identified heat shock transcription factor A3 (HsfA3) as a highly up-regulated heatinducible gene in transgenic plants over-expressing DREB2s (Chen et al, 2010;Yoshida et al, 2008). Moreover, transient promoter reporter assays using mesophyll protoplasts demonstrated that HsfA3 expression is directly regulated by DREB2s under conditions of heat stress (Chen et al, 2010;Schramm et al, 2008) and that DREB2-overexpressing transgenic plants have increased tolerance to heat stress (Almoguera et al, 2009;Lee et al, 2010;Lim et al, 2007;Matsukura et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies identified heat shock transcription factor A3 (HsfA3) as a highly up-regulated heatinducible gene in transgenic plants over-expressing DREB2s (Chen et al, 2010;Yoshida et al, 2008). Moreover, transient promoter reporter assays using mesophyll protoplasts demonstrated that HsfA3 expression is directly regulated by DREB2s under conditions of heat stress (Chen et al, 2010;Schramm et al, 2008) and that DREB2-overexpressing transgenic plants have increased tolerance to heat stress (Almoguera et al, 2009;Lee et al, 2010;Lim et al, 2007;Matsukura et al, 2010). Recent work established that DREB2C physically interacts with ABF2, a bZIP protein regulating abscisic acid (ABA)-responsive gene expression, and its overexpression affected ABA sensitivity .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Arabidopsis Dehydration- Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Hwang

Lim

Chen

et al. 2012

Molecules and Cells

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Dehydration-responsive element-binding proteins (DREBs) regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluorescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H 2 O 2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activatorreporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.

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“…Помимо этого извест-но, что у A. thaliana HSFA1d и HSFA1e совмест-но с HSFA1a и HSFA1b регулируют экспрессию генов HSFA7a, HSFB1 и HSFB2a [von KoskullDöring et al, 2007;Nishizawa-Yokoi et al, 2011]. HSFA1 контролируют экспрессию HSFA2 и DRE-B2A [Liu et al, 2011], HSFA3 индуцирует синтез DREB2С [Chen et al, 2010].…”

Section: передача сигнала о высокотемпературном воздействииunclassified

“…В свою очередь, это приводит к акти-вации экспрессии генов других ТФ и стрес-совых генов [Nakashima et al, 2009;Sun et al, 2014]. Известно, что у A. thaliana DREB2A ре-гулируют экспрессию HSP70, HSP18.1, CPsH-SP, AtCYP18-1 , DREB2A и DREB2C -экспрессию гена HSFA3 [Schramm et al, 2008;Chen et al, 2010;Grover et al, 2013]. Сверхэкспрессия OsDREB2B у A. thaliana при-водит не только к повышению экспрессии гена DREB2A, но и к увеличению теплоустойчивости трансгенных растений [Matsukura et al, 2010].…”

Section: передача сигнала о высокотемпературном воздействииunclassified

Main Stages of Plant Cellular Response to Heat Stress

Nilova

Топчиева

Титов

et al. 2017

Proceedings of KarRC of RAS

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Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response

Cited by 140 publications

References 19 publications

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Overexpression of Arabidopsis Dehydration- Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Main Stages of Plant Cellular Response to Heat Stress

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