Arabidopsis Glutathione-S-Transferases GSTF11 and GSTU20 Function in Aliphatic Glucosinolate Biosynthesis

Zhang, Aiqin; Luo, Rui; Li, Jiawen; Miao, Rongqing; An, Hui Jin; Pang, Qiuying

doi:10.3389/fpls.2021.816233

Cited by 12 publications

(8 citation statements)

References 48 publications

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“…There is strong evidence that glutathione transferase is involved in the regulation of plant stress resistance. For example, GSTFs have been observed in Arabidopsis [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola

Jia,

Ni,

Zhao

et al. 2024

BMC Plant Biol

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Background Corynespora leaf spot is a common leaf disease occurring in sesame, and the disease causes leaf yellowing and even shedding, which affects the growth quality of sesame. At present, the mechanism of sesame resistance to this disease is still unclear. Understanding the resistance mechanism of sesame to Corynespora leaf spot is highly important for the control of infection. In this study, the leaves of the sesame resistant variety (R) and the sesame susceptible variety (S) were collected at 0–48 hpi for transcriptome sequencing, and used a combined third-generation long-read and next-generation short-read technology approach to identify some key genes and main pathways related to resistance. Results The gene expression levels of the two sesame varieties were significantly different at 0, 6, 12, 24, 36 and 48 hpi, indicating that the up-regulation of differentially expressed genes in the R might enhanced the resistance. Moreover, combined with the phenotypic observations of sesame leaves inoculated at different time points, we found that 12 hpi was the key time point leading to the resistance difference between the two sesame varieties at the molecular level. The WGCNA identified two modules significantly associated with disease resistance, and screened out 10 key genes that were highly expressed in R but low expressed in S, which belonged to transcription factors (WRKY, AP2/ERF-ERF, and NAC types) and protein kinases (RLK-Pelle_DLSV, RLK-Pelle_SD-2b, and RLK-Pelle_WAK types). These genes could be the key response factors in the response of sesame to infection by Corynespora cassiicola. GO and KEGG enrichment analysis showed that specific modules could be enriched, which manifested as enrichment in biologically important pathways, such as plant signalling hormone transduction, plant-pathogen interaction, carbon metabolism, phenylpropanoid biosynthesis, glutathione metabolism, MAPK and other stress-related pathways. Conclusions This study provides an important resource of genes contributing to disease resistance and will deepen our understanding of the regulation of disease resistance, paving the way for further molecular breeding of sesame.

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“…There is strong evidence that glutathione transferase is involved in the regulation of plant stress resistance. For example, GSTFs have been observed in Arabidopsis [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola

Jia,

Ni,

Zhao

et al. 2024

BMC Plant Biol

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“…This information could be used to design constructs with adjusted expression levels and suggest further optimization steps with respect to the integration into the host's general metabolism. Attention should also be paid to the involvement of glutathione-Stransferases (GSTs) (Piślewska- Bednarek et al 2018;Zhang et al 2022). The presently used constructs do not contain a GST gene as conjugation of the acinitro compound with glutathione (GSH) seems to be catalyzed by GSTs present in heterologous plant hosts (Mikkelsen et al 2010;Møldrup et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Production of benzylglucosinolate in genetically engineered carrot suspension cultures

Kurzbach

Strieker

Wittstock

2022

Plant Biotechnology

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Glucosinolates, a group of sulfur-containing specialized metabolites of the Brassicales, have attracted a lot of interest in nutrition, medicine and agriculture due to their positive health effects and their involvement in plant defense. Their biological activities and the extensive knowledge of their biosynthesis have inspired research into development of crops with enhanced glucosinolate contents as well as their biotechnological production in homologous and heterologous systems. Here, we provide proof-of-concept for transgenic suspension cultures of carrot (Daucus carota, Apiacae) as a scalable production platform for plant specialized metabolites using benzylglucosinolate as a model. Two T-DNAs carrying in total six genes of the benzylglucosinolate biosynthesis pathway from Arabidopsis thaliana as well as NPTII and BAR as selectable markers were transferred to carrot cells by Agrobacterium tumefaciens-mediated transformation. Putative transformants selected based on their kanamycin and BASTA resistances were subjected to HPLC-MS analysis. Of 79 putative transformants, 17 produced benzylglucosinolate. T-DNA-integration was confirmed for the five best producers. Callus from these transformants was used to establish suspension cultures for quantitative analysis. When grown in 60-mlcultures, the best transformants produced roughly 2.5 nmol (g fw) −1 benzylglucosinolate, together with up to 10 nmol (g fw) −1 desulfobenzylglucosinolate. Only one transformant produced more benzylglucosinolate than desulfobenzylglucosinolate. The concentration of sulfate in the medium was not a major limiting factor. High production seemed to be associated with poor growth and vice versa. Therefore, future research should try to optimize medium and cultivation process and to separate growth and production phase by using an inducible promoter.

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“…(2003) CYP79B2CYP79B3CYP83B1 OE B. rapa N root Zang et al. (2009) GSTF11 KO A. thaliana N N N N – N leaf/seed Zhang et al. (2022) GSTU20 KO A. thaliana N N N N – N leaf/seed Zhang et al.…”

Section: Candidate Targets For Current and Future Gsl Metabolic Engin...mentioning

confidence: 99%

Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

Qin

King

Borpatragohain

et al. 2023

Plant Communications

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Arabidopsis Glutathione-S-Transferases GSTF11 and GSTU20 Function in Aliphatic Glucosinolate Biosynthesis

Cited by 12 publications

References 48 publications

Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola

Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola

Production of benzylglucosinolate in genetically engineered carrot suspension cultures

Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

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