Arabidopsis MKK10-MPK6 mediates red-light-regulated opening of seedling cotyledons through phosphorylation of PIF3

Xin, Xiaoyun; Chen, Wenhao; Wang, Bo; Zhu, Fan; Li, Yuan; Yang, Hongqiang; Li, Jigang; Ren, Dongtao

doi:10.1093/jxb/erx418

Cited by 40 publications

(27 citation statements)

References 89 publications

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“…Additional protein kinases have been implicated in PIF phosphorylation and subsequent proteasome-mediated degradation including BIN2, CK2, and MPK6 (refs. 73,76–78 ) (reviewed in ref. 41 ).…”

Section: Phytochrome Signaling Elicited Through Light-activated Intermentioning

confidence: 99%

Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants

2019

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Phytochromes are bilin-binding photosensory receptors which control development over a broad range of environmental conditions and throughout the whole plant life cycle. Light-induced conformational changes enable phytochromes to interact with signaling partners, in particular transcription factors or proteins that regulate them, resulting in large-scale transcriptional reprograming. Phytochromes also regulate promoter usage, mRNA splicing and translation through less defined routes. In this review we summarize our current understanding of plant phytochrome signaling, emphasizing recent work performed in Arabidopsis. We compare and contrast phytochrome responses and signaling mechanisms among land plants and highlight open questions in phytochrome research.

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Section: Phytochrome Signaling Elicited Through Light-activated Intermentioning

confidence: 99%

Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants

2019

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“…Upon exposure to light, PIFs are phosphorylated and degraded. Recently, Xin et al reported the involvement of Arabidopsis MKK10-MPK6 module in regulating cotyledon opening upon red light exposure (Xin et al, 2018 ). Moreover, MPK6 interacts with and phosphorylates PIF3 leading to its degradation.…”

Section: Developmentmentioning

confidence: 99%

“…Moreover, MPK6 interacts with and phosphorylates PIF3 leading to its degradation. Collectively, the results of Xin et al suggest that a module composed of MKK10-MPK6-PIF3 regulates seedling photomorphogenesis in response to red light (Xin et al, 2018 ).…”

Section: Developmentmentioning

confidence: 99%

Nuclear Signaling of Plant MAPKs

2018

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Mitogen-activated protein kinases (MAPKs) are conserved protein kinases in eukaryotes that establish signaling modules where MAPK kinase kinases (MAPKKKs) activate MAPK kinases (MAPKKs) which in turn activate MAPKs. In plants, they are involved in the signaling of multiple environmental stresses and developmental programs. MAPKs phosphorylate their substrates and this post-translational modification (PTM) contributes to the regulation of proteins. PTMs may indeed modify the activity, subcellular localization, stability or trans-interactions of modified proteins. Plant MAPKs usually localize to the cytosol and/or nucleus, and in some instances they may also translocate from the cytosol to the nucleus. Upon the detection of environmental changes at the cell surface, MAPKs participate in the signal transduction to the nucleus, allowing an adequate transcriptional reprogramming. The identification of plant MAPK substrates largely contributed to a better understanding of the underlying signaling mechanisms. In this review, we highlight the nuclear signaling of plant MAPKs. We discuss the activation, regulation and activity of plant MAPKs, as well as their nuclear re-localization. We also describe and discuss known nuclear substrates of plant MAPKs in the context of biotic stress, abiotic stress and development and consider future research directions in the field of plant MAPKs.

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“…BIN2 has also been shown to regulate the phosphorylation and transcriptional activity of PIF4 in diurnal conditions (14). Recently, there is evidence showing that phytochromes, Photoregulatory Protein Kinases (PPKs), and MPK6 can also phosphorylate PIF3 proteins (18)(19)(20). In contrast to the extensive studies of PIF protein phosphorylation, dephosphorylation of PIF proteins is poorly understood.…”

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confidence: 99%

Arabidopsis PP6 phosphatases dephosphorylate PIF proteins to repress photomorphogenesis

Dong

Deng

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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The PHYTOCHROME-INTERACTING FACTORs (PIFs) play a central role in repressing photomorphogenesis, and phosphorylation mediates the stability of PIF proteins. Although the kinases responsible for PIF phosphorylation have been extensively studied, the phosphatases that dephosphorylate PIFs remain largely unknown. Here, we report that seedlings with mutations in FyPP1 and FyPP3, 2 genes encoding the catalytic subunits of protein phosphatase 6 (PP6), exhibited short hypocotyls and opened cotyledons in the dark, which resembled the photomorphogenic development of dark-grown pifq mutants. The hypocotyls of dark-grown sextuple mutant fypp1 fypp3 (f1 f3) pifq were shorter than those of parental mutants f1 f3 and pifq, indicating that PP6 phosphatases and PIFs function synergistically to repress photomorphogenesis in the dark. We showed that FyPPs directly interacted with PIF3 and PIF4, and PIF3 and PIF4 proteins exhibited mobility shifts in f1 f3 mutants, consistent with their hyperphosphorylation. Moreover, PIF4 was more rapidly degraded in f1 f3 mutants than in wild type after light exposure. Whole-genome transcriptomic analyses indicated that PP6 and PIFs coregulated many genes, and PP6 proteins may positively regulate PIF transcriptional activity. These data suggest that PP6 phosphatases may repress photomorphogenesis by controlling the stability and transcriptional activity of PIF proteins via regulating PIF phosphorylation.

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Arabidopsis MKK10-MPK6 mediates red-light-regulated opening of seedling cotyledons through phosphorylation of PIF3

Abstract: The kinases responsible for phosphorylation of phytochrome-interacting factors (PIFs) in plants undergoing photomorphogenesis are largely unknown. We show that the MKK10-MPK6 cascade regulates red light-induced photomorphogenesis through phosphorylation of PIF3.

Cited by 40 publications

References 89 publications

Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants

Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants

Nuclear Signaling of Plant MAPKs

Arabidopsis PP6 phosphatases dephosphorylate PIF proteins to repress photomorphogenesis

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