Arabidopsis mutants affected in the transcriptional control of allene oxide synthase, the enzyme catalyzing the entrance step in octadecanoid biosynthesis

Kubigsteltig, Ines; Weiler, Elmar W.

doi:10.1007/s00425-003-1056-x

Cited by 25 publications

(18 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1998; Mei et al. 2006) is much lower than that of Arabidopsis (basal level of 10–100 ng·g −1 FW and 1000–2000 ng·g −1 FW after wounding; Kubigsteltig & Weiler 2003). In the current study, we have evaluated the systemic defence response in the monocotyledon O. sativa after infection with two nematode species with very different life styles: the root knot nematode M. graminicola and the root rot nematode H. oryzae .…”

Section: Discussionmentioning

confidence: 87%

Comparing systemic defence‐related gene expression changes upon migratory and sedentary nematode attack in rice

et al. 2011

View full text Add to dashboard Cite

Complex defence signalling pathways, controlled by different hormones, are known to be involved in the reaction of plants to a wide range of biotic and abiotic stress factors. Here, we studied the differential expression of genes involved in stress and defence responses in systemic tissue of rice infected with the root knot nematode (RKN) Meloidogyne graminicola and the migratory root rot nematode Hirschmanniella oryzae, two agronomically important rice pathogens with very different lifestyles. qRT-PCR revealed that all investigated systemic tissues had significantly lower expression of isochorismate synthase, a key enzyme for salicylic acid production involved in basal defence and systemic acquired resistance. The systemic defence response upon migratory nematode infection was remarkably similar to fungal rice blast infection. Almost all investigated defence-related genes were up-regulated in rice shoots 3 days after root rot nematode attack, including the phenylpropanoid pathway, ethylene pathway and PR genes, but many of which were suppressed at 7 dpi. Systemic shoot tissue of RKN-infected plants showed similar attenuation of expression of almost all studied genes already at 3 dpi, with clear attenuation of the ethylene pathway and methyl jasmonate biosynthesis. These results provide an interesting starting point for further studies to elucidate how nematodes are able to suppress systemic plant defence mechanisms and the effect in multitrophic interactions.

show abstract

Section: Discussionmentioning

confidence: 87%

Comparing systemic defence‐related gene expression changes upon migratory and sedentary nematode attack in rice

et al. 2011

View full text Add to dashboard Cite

show abstract

“…4C). Conversely, JA-hypersensitive mutant iop1 (Penninckx et al, 2003) and the constitutive JA-producing mutant cas1 (Kubigsteltig and Weiler, 2003) were more tolerant to selenite than their wild type, C24 (Fig. 4C).…”

Section: Resultsmentioning

confidence: 96%

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

2008

View full text Add to dashboard Cite

Selenium (Se) is an essential element for many organisms, but excess Se is toxic. To better understand plant Se toxicity and resistance mechanisms, we compared the physiological and molecular responses of two Arabidopsis (Arabidopsis thaliana) accessions, Columbia (Col)-0 and Wassilewskija (Ws)-2, to selenite treatment. Measurement of root length Se tolerance index demonstrated a clear difference between selenite-resistant Col-0 and selenite-sensitive Ws-2. Macroarray analysis showed more pronounced selenite-induced increases in mRNA levels of ethylene-or jasmonic acid (JA)-biosynthesis and -inducible genes in Col-0 than in Ws-2. Indeed, Col-0 exhibited higher levels of ethylene and JA. The selenite-sensitive phenotype of Ws-2 was attenuated by treatment with ethylene precursor or methyl jasmonate (MeJA). Conversely, the selenite resistance of Col-0 was reduced in mutants impaired in ethylene or JA biosynthesis or signaling. Genes encoding sulfur (S) transporters and S assimilation enzymes were up-regulated by selenite in Col-0 but not Ws-2. Accordingly, Col-0 contained higher levels of total S and Se and of nonprotein thiols than Ws-2. Glutathione redox status was reduced by selenite in Ws-2 but not in Col-0. Furthermore, the generation of reactive oxygen species by selenite was higher in Col-0 than in Ws-2. Together, these results indicate that JA and ethylene play important roles in Se resistance in Arabidopsis. Reactive oxygen species may also have a signaling role, and the resistance mechanism appears to involve enhanced S uptake and reduction.

show abstract

“…If JAs positively modulate abscission it is likely that they act indirectly or, like HAE, act very early in the abscission pathway. However, constitutive expression of the sole allene oxide synthase gene in Arabidopsis did not lead to precocious floral organ abscission in studies of Kubigsteltig and Weiler (2003). There, floral parts of the cas AOS overexpresser persisted along much of the inflorescence.…”

Section: Cluster 7 Contains Multiple Down-regulated Genes Of Ja Biosymentioning

confidence: 99%

Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing ArabidopsisZINC FINGER PROTEIN2

2008

View full text Add to dashboard Cite

Organ detachment requires cell separation within abscission zones (AZs). Physiological studies have established that ethylene and auxin contribute to cell separation control. Genetic analyses of abscission mutants have defined ethylene-independent detachment regulators. Functional genomic strategies leading to global understandings of abscission have awaited methods for isolating AZ cells of low abundance and very small size. Here, we couple laser capture microdissection of Arabidopsis thaliana stamen AZs and GeneChip profiling to reveal the AZ transcriptome responding to a developmental shedding cue. Analyses focus on 551 AZ genes (AZ 551 ) regulated at the highest statistical significance (P # 0.0001) over five floral stages linking prepollination to stamen shed. AZ 551 includes mediators of ethylene and auxin signaling as well as receptor-like kinases and extracellular ligands thought to act independent of ethylene. We hypothesized that novel abscission regulators might reside in disproportionately represented Gene Ontology Consortium functional categories for cell wall modifying proteins, extracellular regulators, and nuclear-residing transcription factors. Promoter-b-glucuronidase expression of one transcription factor candidate, ZINC FINGER PROTEIN2 (AtZFP2), was elevated in stamen, petal, and sepal AZs. Flower parts of transgenic lines overexpressing AtZFP2 exhibited asynchronous and delayed abscission. Abscission defects were accompanied by altered floral morphology limiting pollination and fertility. Hand-pollination restored transgenic fruit development but not the rapid abscission seen in wild-type plants, demonstrating that pollination does not assure normal rates of detachment. In wild-type stamen AZs, AtZFP2 is significantly up-regulated postanthesis. Phenotype data from transgene overexpression studies suggest that AtZFP2 participates in processes that directly or indirectly influence organ shed.

show abstract

Arabidopsis mutants affected in the transcriptional control of allene oxide synthase, the enzyme catalyzing the entrance step in octadecanoid biosynthesis

Cited by 25 publications

References 50 publications

Comparing systemic defence‐related gene expression changes upon migratory and sedentary nematode attack in rice

Comparing systemic defence‐related gene expression changes upon migratory and sedentary nematode attack in rice

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing ArabidopsisZINC FINGER PROTEIN2

Contact Info

Product

Resources

About