Arabidopsis thaliana peroxidases involved in lignin biosynthesis: In silico promoter analysis and hormonal regulation

Herrero, Joaquín; Esteban, Alberto Díaz; Zapata, José Miguel

doi:10.1016/j.plaphy.2014.03.027

Cited by 32 publications

(22 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…From our data, we may speculate that CKs regulate the initiation of lignin deposition in secondary tissues, while later lignification is tuned by other factors. AtPRX52 (whose hemp ortholog was more expressed in H20, Supplementary Table S6) is an example of these genes regulated by several factors: it contains a high number of Arabidopsis response regulator 1 (ARR1)-cytokinin-binding element in its promoter region, but is also responsive to bZIP9, bZIP50, NST1, MYB46 , or MYB85 (Herrero et al, 2014). All these TFs were, notably, more abundant either in H15 or in H20 (Supplementary Table S4).…”

Section: Discussionmentioning

confidence: 99%

Studying Secondary Growth and Bast Fiber Development: The Hemp Hypocotyl Peeks behind the Wall

et al. 2016

View full text Add to dashboard Cite

Cannabis sativa L. is an annual herbaceous crop grown for the production of long extraxylary fibers, the bast fibers, rich in cellulose and used both in the textile and biocomposite sectors. Despite being herbaceous, hemp undergoes secondary growth and this is well exemplified by the hypocotyl. The hypocotyl was already shown to be a suitable model to study secondary growth in other herbaceous species, namely Arabidopsis thaliana and it shows an important practical advantage, i.e., elongation and radial thickening are temporally separated. This study focuses on the mechanisms marking the transition from primary to secondary growth in the hemp hypocotyl by analysing the suite of events accompanying vascular tissue and bast fiber development. Transcriptomics, imaging and quantification of phytohormones were carried out on four representative developmental stages (i.e., 6–9–15–20 days after sowing) to provide a comprehensive overview of the events associated with primary and secondary growth in hemp. This multidisciplinary approach provides cell wall-related snapshots of the growing hemp hypocotyl and identifies marker genes associated with the young (expansins, β-galactosidases, and transcription factors involved in light-related processes) and the older hypocotyl (secondary cell wall biosynthetic genes and transcription factors).

show abstract

Section: Discussionmentioning

confidence: 99%

Studying Secondary Growth and Bast Fiber Development: The Hemp Hypocotyl Peeks behind the Wall

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Our results suggest that the main regulatory point occurs at the oxidative polymerization step and that the typical low lignification observed in wild-type bast fibers is related to the absence of different actors necessary for monolignol oxidation. Recent analyses of peroxidase gene promoters have suggested that these genes are regulated by a number of different transcription factors (NAC, MYB, AP2, and class I and III HD-ZIP) previously associated with vascular tissue formation and/or secondary cell wall formation (Herrero et al, 2014), and it is possible that increased bast fiber lignification is associated with a mutation in such a gene(s). Alternatively, peturbations in the biosynthesis of other cell wall polymers affecting cell wall integrity and/or activation of defense signaling could also be responsible for the ectopic lignification in the lbf1 mutant.…”

Section: Discussionmentioning

confidence: 99%

Ectopic Lignification in the Flax lignified bast fiber1 Mutant Stem Is Associated with Tissue-Specific Modifications in Gene Expression and Cell Wall Composition

et al. 2014

View full text Add to dashboard Cite

Histochemical screening of a flax ethyl methanesulfonate population led to the identification of 93 independent M2 mutant families showing ectopic lignification in the secondary cell wall of stem bast fibers. We named this core collection the Linum usitatissimum (flax) lbf mutants for lignified bast fibers and believe that this population represents a novel biological resource for investigating how bast fiber plants regulate lignin biosynthesis. As a proof of concept, we characterized the lbf1 mutant and showed that the lignin content increased by 350% in outer stem tissues containing bast fibers but was unchanged in inner stem tissues containing xylem. Chemical and NMR analyses indicated that bast fiber ectopic lignin was highly condensed and rich in G-units. Liquid chromatography-mass spectrometry profiling showed large modifications in the oligolignol pool of lbf1 innerand outer-stem tissues that could be related to ectopic lignification. Immunological and chemical analyses revealed that lbf1 mutants also showed changes to other cell wall polymers. Whole-genome transcriptomics suggested that ectopic lignification of flax bast fibers could be caused by increased transcript accumulation of (1) the cinnamoyl-CoA reductase, cinnamyl alcohol dehydrogenase, and caffeic acid O-methyltransferase monolignol biosynthesis genes, (2) several lignin-associated peroxidase genes, and (3) genes coding for respiratory burst oxidase homolog NADPH-oxidases necessary to increase H 2 O 2 supply.

show abstract

“…Several studies assert that peroxidase and phenolic compounds are involved in tissue lignification (Hiraga et al, 2001;Liu, 2012), which are important in the early stages of the connection between the graft and rootstock (Irisarri et al, 2015), because the cell walls of xylem tissues are dynamic structures composed of polysaccharides, proteins, minerals and phenolic compounds such as lignin (Herrero et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Can early peroxidase quantification detect graft-compatible in anonaceous rootstocks?

Báron¹,

Amaro²,

Macedo³

et al. 2018

Afr. J. Agric. Res.

View full text Add to dashboard Cite

This study aimed to quantify the class III peroxidase activity in young plants of different species belonging to Annonaceae botany family, with high potential as a rootstock, for early detection of graftcompatible atemoya (Annona x atemoya Mabb.). The experimental design was randomized block, that evaluated the species araticum-de-terra-fria (Annona emarginata (Schltdl.) H. Rainer 'variety terra-fria'), araticum-mirim (Annona emarginata (Schltdl.) H. Rainer 'variety mirim) and biribá (Annona mucosa (Schltdl.) H. Rainer), with rootstock potential, and atemoya (scion), which were divided into four blocks, each with four plants. The peroxidase was quantified on the stem before grafting. Statistical analysis showed that only the biribá presented different peroxidase activity compared to atemoya plants; and araticum-mirim and araticum-de-terra-fria were similar to atemoya plants. Thus, the peroxidase activity could not be possible or be used as a tool in the early diagnosis of the graft-compatible in atemoya combinations.

show abstract

Arabidopsis thaliana peroxidases involved in lignin biosynthesis: In silico promoter analysis and hormonal regulation

Cited by 32 publications

References 40 publications

Studying Secondary Growth and Bast Fiber Development: The Hemp Hypocotyl Peeks behind the Wall

Studying Secondary Growth and Bast Fiber Development: The Hemp Hypocotyl Peeks behind the Wall

Ectopic Lignification in the Flax lignified bast fiber1 Mutant Stem Is Associated with Tissue-Specific Modifications in Gene Expression and Cell Wall Composition

Can early peroxidase quantification detect graft-compatible in anonaceous rootstocks?

Contact Info

Product

Resources

About