Arabinogalactan proteins at the cell surface of Brassica sperm and Lilium sperm and generative cells

Southworth, Darlene; Kwiatkowski, Stephen

doi:10.1007/s004970050042

Cited by 23 publications

(28 citation statements)

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“…Moreover, after the second pollen mitosis, the two resulting sperm cells that are inside the pollen grain or inside the pollen tube are still strongly labelled by these two mAbs. The specific labelling of the generative cell was also reported for oilseed rape (Pennell et al, 1991), for Nicotiana tabacum (Li et al, 1995) and for Brassica campestris male gametes (Southworth & Kwiatkowski, 1996). JIM8 and JIM13 do not label the pollen tube wall, which instead is labelled by MAC207 and LM2 (Pereira et al, 2006).…”

Section: Immunolocalization Of Glycan Epitopesmentioning

confidence: 55%

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

Coimbra¹,

Pereira²

2012

Transgenic Plants - Advances and Limitations

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Section: Immunolocalization Of Glycan Epitopesmentioning

confidence: 55%

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

Coimbra¹,

Pereira²

2012

Transgenic Plants - Advances and Limitations

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“…Previous studies have shown that AGPs are expressed in pollen or tapetum in several plant species and may be related to signal transduction between the tapetum and immature pollen (Southworth and Kwiatkowski, 1996;Scutt and Gilmartin, 1998). Seven genes, encoding AGP1, -6, -11, -15, -23, -24, and -40, have been identified as RGA down-regulated genes in our study (Supplemental Table S2), and six of these genes are specifically or predominantly expressed in stamens (Supplemental Table S7).…”

Section: Rga Predominantly Regulates Stamen and Pollen Developmentmentioning

confidence: 99%

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Hou

Shen

et al. 2008

Plant Physiology

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Gibberellin (GA) plays important roles in regulating many aspects of plant development. GA derepresses its signaling pathway by promoting the degradation of DELLA proteins, a family of nuclear growth repressors. Although the floral organ identity is established in flowers of the GA-deficient mutant ga1-3, the growth of all floral organs is severely retarded. In particular, abortive anther development in ga1-3 results in male sterility. Genetic analysis has revealed that various combinations of null mutants of DELLA proteins could gradually rescue floral organ defects in ga1-3 and that RGA is the most important DELLA protein involved in floral organ development. To elucidate the early molecular events controlled by RGA during flower development, we performed whole-genome microarray analysis to identify genes in response to the steroid-inducible activation of RGA in ga1-3 rgl2 rga 35S:RGA-GR. Although DELLA proteins were suggested as transcriptional repressors, similar numbers of genes were down-regulated or up-regulated by RGA during floral organ development. More than one-third of RGA down-regulated genes were specifically or predominantly expressed in stamens. A significant number of RGA-regulated genes are involved in phytohormone signaling or stress response. Further expression analysis through activation of RGA by steroid induction combined with cycloheximide identified eight genes as immediate targets of RGA. In situ hybridization and transgenic studies further showed that the expression pattern and function of several selected genes were consistent with the predictions from microarray analysis. These results suggest that DELLA regulation of floral organ development is modulated by multiple phytohormones and stress signaling pathways.

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“…Presumably, sperm maturation involves multiple carbohydrate determinants on the cell surface that reduce the likelihood of random fusions but that do not impede sexual gamete fusion. Such surface changes are anticipated to include compounds such as arabinogalactan proteins (Southworth and Kwiatkowski, 1996) and other potentially immunogenic compounds (Southworth et al, 1999). In Plumbago, the polarity of the male germ unit is determined by the position of the vegetative nucleus and appears to be determined in the generative cell .…”

Section: Sperm Maturationmentioning

confidence: 99%