Arachidonic acid promotes FAK activation and migration in MDA-MB-231 breast cancer cells

Navarro-Tito, Napoleón; Robledo, Teresa; Salazar, Eduardo Pérez

doi:10.1016/j.yexcr.2008.08.018

Cited by 57 publications

(51 citation statements)

References 79 publications

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“…In the profiled spectrum from negative ion mode, distinctive fatty acids and lipids were identified in breast cancer tissues. Studies indicated that mediumand long-chain free fatty acids are implicated in the activation of G protein-coupled receptors expressed in breast cancer cell lines (69)(70)(71). Some of these lipids are also known to be involved in the migration, proliferation, and invasion of breast cancer cells (i.e., oleic acid) or more generally in tumor processes (i.e., arachidonic acid) (69,(71)(72)(73)(74).…”

Section: Discussionmentioning

confidence: 99%

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Calligaris

Caragacianu

Liu

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

216

202

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Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used.Here, we present a proof-of-concept study to determine the feasibility of using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) for identifying and differentiating tumor from normal breast tissue. We show that tumor margins can be identified using the spatial distributions and varying intensities of different lipids. Several fatty acids, including oleic acid, were more abundant in the cancerous tissue than in normal tissues. The cancer margins delineated by the molecular images from DESI-MSI were consistent with those margins obtained from histological staining. Our findings prove the feasibility of classifying cancerous and normal breast tissues using ambient ionization MSI. The results suggest that an MSbased method could be developed for the rapid intraoperative detection of residual cancer tissue during breast-conserving surgery.B reast cancer is the most commonly diagnosed carcinoma in women in the United States and Western countries. Breast conservation surgery (BCS) has become the preferred treatment option for many women with early-stage breast cancer (1). BCS entails resection of the tumor, with a clean margin of normal tissue around it. Surgery is usually followed by radiation therapy. Results from seven large randomized prospective studies, with the largest two having over 20 y of follow-up, have shown equal survival when comparing BCS coupled with whole-breast radiation and mastectomy (2, 3).Normally, breast surgeons aim to remove a patient's tumor, along with a rim of normal tissue that is free of cancer. Preoperative mammography, ultrasonography, or MRI may be used by the surgeon to guide adequate resection (4-6). Despite numerous improvements in imaging and surgical technique, the need for reexcision to achieve complete tumor resection in the United States typically ranges from 20-40% (7-15), and has been reported as being as high as 60% (16). The importance of reexcision is underscored by numerous studies, which have shown that incomplete resection of tumor and positive margins are associated with increased locoregional recurrence compared with negative margins (12,(17)(18)(19)(20). Furthermore, the landmark meta-analysis performed by the Early Breast Cancer Trialists' Collaborative Group (18, 21) directly linked local recurrence to survival, placing great emphasis on the surgeon's role in minimizing local recurrence by obtaining adequate margins.Breast tumor reexcisions are accompanied by a number of undesirable problems: The completion of therapy is delayed, infection rates are increased, cost is increased, there can be a negative psychological impact on the patient, and there can be diminished aesthetic outcomes (22-24). The development of an intraoperative technique that allows the fast and accurate ident...

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Section: Discussionmentioning

confidence: 99%

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Calligaris

Caragacianu

Liu

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

216

202

View full text Add to dashboard Cite

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“…However, inhibition of p38 did partially prevent PKCμ cleavage by calpain [76]. Finally, inhibition of the lipooxygenases or src prevented the increase in migration due to arachidonate [66].…”

Section: Optimizing Dietary Fatmentioning

confidence: 92%

“…Treatment of MDA-MB-231 breast cancer cells with 100-400 μM oleate promotes cell migration by approximately 2-4.7 fold. Further molecular experiments revealed this migration was induced by oleate-mediated production of arachidonate, which in turn activated focal adhesion kinase (FAK) [66]. Furthermore, 500 μM oleate induced invasion of MDA-MB-231 cells through Matrigel by approximately 2 fold [67].…”

Section: Oleatementioning

confidence: 97%

Optimizing Dietary Fat to Reduce Breast Cancer Risk: Are we there Yet?

Evans¹,

Hardy²

2014

TOBCANJ

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For over 70 years, long chain fatty acids have been implicated in the development and progression of breast cancer. Although the exact role remains to be elucidated, dietary factors have been implicated in approximately 35% of cancer deaths. Currently, biomarker, animal and in vitro studies suggest that the individual fatty acids have differing roles in the promotion or prevention of breast cancer development and progression. The goal of this review is to assess epidemiological, animal and cellular studies with respect to the role of dietary long chain fatty acids in breast cancer risk. Subsequently we identify the common findings in these studies, discuss important factors that may influence human studies and evaluate the current dietary fat recommendations with respect to these findings.

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“…In NIH3T3 mouse fibroblasts, the initial transient burst in AA resulting from cPLA 2 activation is responsible for cell adhesion, with the AA metabolites leukotrienes and PGs regulating cell spreading and cell migration, respectively [93][94][95]. Other reports have shown that cPLA 2 α and its Ser-505-phosphorylated form are rapidly and specifically recruited at sites of actin polymerization such as membrane ruffles and leading edges [92].…”

Section: Cytosolic Phospholipase A2mentioning

confidence: 99%

Interaction of brain fatty acid-binding protein with the polyunsaturated fatty acid environment as a potential determinant of poor prognosis in malignant glioma

Elsherbiny

Emara

Godbout

2013

Progress in Lipid Research

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Malignant gliomas are the most common adult brain cancers. In spite of aggressive treatment, recurrence occurs in the great majority of patients and is invariably fatal. Polyunsaturated fatty acids are abundant in brain, particularly ω-6 arachidonic acid (AA) and ω-3 docosahexaenoic acid (DHA). Although the levels of ω-6 and ω-3 polyunsaturated fatty acids are tightly regulated in brain, the ω-6:ω-3 ratio is dramatically increased in malignant glioma, suggesting deregulation of fundamental lipid homeostasis in brain tumor tissue. The migratory properties of malignant glioma cells can be modified by altering the ratio of AA:DHA in growth medium, with increased migration observed in AA-rich medium. This fatty acid-dependent effect on cell migration is dependent on expression of the brain fatty acid binding protein (FABP7) previously shown to bind DHA and AA. Increased levels of enzymes involved in eicosanoid production in FABP7-positive malignant glioma cells suggest that FABP7 is an important modulator of AA metabolism. We provide evidence that increased production of eicosanoids in FABP7-positive malignant glioma growing in an AA-rich environment contributes to tumor infiltration in the brain. We discuss pathways and molecules that may underlie FABP7/AA-mediated promotion of cell migration and FABP7/DHA-mediated inhibition of cell migration in malignant glioma.

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Arachidonic acid promotes FAK activation and migration in MDA-MB-231 breast cancer cells

Cited by 57 publications

References 79 publications

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Optimizing Dietary Fat to Reduce Breast Cancer Risk: Are we there Yet?

Interaction of brain fatty acid-binding protein with the polyunsaturated fatty acid environment as a potential determinant of poor prognosis in malignant glioma

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