Arbuscular mycorrhizal technology for the growth enhancement of micropropagated Spilanthes acmella Murr

Yadav, Kuldeep; Singh, Narender; Aggarwal, Ashok

doi:10.17221/21/2011-pps

Cited by 21 publications

(10 citation statements)

References 20 publications

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“…In this research, the well-developed and rooted plantlets were also supported by AM fungi inocula. Concerning ex vitro survival, all the mycorrhizal treatments showed higher ex vitro survival than the control plantlets, which is in line with earlier findings [45][46][47]. Adding AM fungi during the acclimatization stage of micropropagation enhances the growth and development of micropropagated plantlets [48,49].…”

Section: Discussionsupporting

confidence: 89%

Micropropagation Supports Reintroduction of an Apulian Artichoke Landrace in Sustainable Cropping Systems

Ancona

Mastro

Jenderek³

et al. 2021

Agronomy

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Artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori) is a perennial plant of the Asteraceae’s family native to the Mediterranean basin. Italy has rich artichoke biodiversity, but many landraces are subjected to genetic erosion caused by increasing use of commercial varieties that are more homogenous in production. An Apulian landrace ‘Troianella’ was established in vitro to valorize and provide high-quality material for propagation in nurseries and, subsequently, for cultivation in production fields. The shoot proliferation was tested on four different growth media, adding cytokinin (-6-benzylamminopurine (BAP-0.05 mg L−1). Among these, the best results were achieved on MS519-A and BM media in which MS macronutrients were supplemented with additional doses of CaCl2 and MgSO4 (plus 120 mg L−1 and 190 mg L−1, respectively). In vitro root induction was obtained with 10 mg L-1 of Indole-3-acetic acid (IAA) and 30 g L−1 of sucrose. Plants derived from tissue culture were acclimatized in greenhouse using mycorrhizal symbiosis to increase survival during the acclimatization phase and to improve their performance after transplanting in field. Three arbuscular mycorrhizal (AM) fungi (Septoglomus viscosum, Funelliformis mosseae, and Symbivit, a commercial mix) were added to a sterile substrate and compared to a sterile control without any AM fungal inocula. After 3 months, the best growth and plant appearance were on substrates with S. viscosum fungus or the commercial mycorrhizal fungi mix. The results supported a development of an efficient micropropagation protocol and a production of high quality plant material for sustainable farming of the endangered ’Troianella’ landrace.

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Section: Discussionsupporting

confidence: 89%

Micropropagation Supports Reintroduction of an Apulian Artichoke Landrace in Sustainable Cropping Systems

Ancona

Mastro

Jenderek³

et al. 2021

Agronomy

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“…Here, the R. fasciculatus-treated plant shows significant growth than B. megaterium-treated plant, which may confirm the vital role of AM fungal. At the same time, the plants inoculated with R. fasciculatus and B. megaterium (T4) had higher growth and ash biomass and total chlorophyll content which correlated with the findings of Joshee et al, [45], Sharma et al [46] and Yadav et al [47].…”

Section: Discussionsupporting

confidence: 85%

Establishment of in vitro protocol and impact of mycorrhization with phosphobacteria on micro propagated Pogostemon mollis Benth. (Lamiaceae)

Muthuraj¹,

Kaffoor²,

Nagarajan³

2018

Journal of Taibah University for Science

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Plants and microbes compete for survival and their organized interactions play a vital role in adapting to stress environments. Microbes have a positive impact on transplanting performance of in vitro propagated plantlets. The present study aims at survival development of micropropagated Pogostemon mollis through microbial inoculation. The leaf and stem explants on MS medium with different concentrations and combinations of BAP, Kn, NAA, IAA and IBA used for the establishment of plantlet. The survival rate of plantlets was successfully increased from 71% to about 70.21% by the inoculation of Rhizophagus fasciculatus and Bacillus megaterium. This inoculation also clearly increases the growth, biomass and chlorophyll of in vitro derived platelets. The present protocol emphasizes the need of bio-hardening on micropropagated plants, particularly the mycorrhization along with phosphobacteria.

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“…AMFs increased the shoot dry biomass yield at the aforementioned doses; this outcome can be related to the well-developed root system of the inoculated plants, a fact that has consequently improved water and nutrient absorption (Augé et al, 2016). Yadav et al (2012) assessed the influence of two AMF species on the development of micropropagated seedlings and observed that variables plant height, number of branches, leaf area, dry matter mass production and chlorophyll content were higher in seedlings inoculated with AMFs than in non-inoculated plants. They reported the growth and survival of seedlings inoculated with AMFs likely due to increased nutrient absorption.…”

Section: Resultsmentioning

confidence: 96%

Arbuscular mycorrhizal fungi and phosphorus in spilanthol and phenolic compound yield in jambu plants

et al. 2021

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Jambu has spilanthol and phenolic compounds that are responsible for several medicinal properties. Besides improving nutrient absorption, arbuscular mycorrhizal fungi (AMF) can change secondary metabolites’ yield. Thus, the aim of the current study was to evaluate AMF inoculation and P influence on shoot dry biomass yield, macronutrient content (N, P, K, Ca and Mg), total phenol contents and spilanthol percentage in jambu plants. The experiment followed a randomized block design at 3x4 factorial arrangement, with two AMF (Rhizophagus clarus and Claroideoglomus etunicatum) and without AMF, at four P doses (0, 30, 60 and 90 mg/kg of soil), with four replicates. Plants inoculated with R. clarus and C. etunicatum, grown without phosphate fertilization, presented increase by 23 and 12 times, respectively, in comparison to plants without AMF. The relative spilanthol area in plants inoculated with R. clarus, without phosphate fertilization, reached 2.4%. Shoot dry biomass yield of jambu plants and the P, K, Ca and Mg contents were higher when plants were inoculated with R. clarus at the lowest P doses. The AMF also increased the contents of total phenols in plants fertilized with 60 mg kg-1. This P dose increased spilanthol concentration in jambu plants inoculated with C. etunicatum.

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Arbuscular mycorrhizal technology for the growth enhancement of micropropagated Spilanthes acmella Murr

Cited by 21 publications

References 20 publications

Micropropagation Supports Reintroduction of an Apulian Artichoke Landrace in Sustainable Cropping Systems

Micropropagation Supports Reintroduction of an Apulian Artichoke Landrace in Sustainable Cropping Systems

Establishment of in vitro protocol and impact of mycorrhization with phosphobacteria on micro propagated Pogostemon mollis Benth. (Lamiaceae)

Arbuscular mycorrhizal fungi and phosphorus in spilanthol and phenolic compound yield in jambu plants

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