Arbutus unedo prevents cardiovascular and morphological alterations in L-NAME-induced hypertensive rats

Afkir, Saida; Nguelefack, Télesphore Benoît; Aziz, Mohammed; Zoheir, Johar; Cuisinaud, G.; Bnouham, Mohamed; Mekhfi, Hassane; Legssyer, Abdelkhaleq; Lahlou, Saad; Ziyyat, Abderrahim

doi:10.1016/j.jep.2007.11.029

Cited by 72 publications

(25 citation statements)

References 37 publications

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“…It is well known that L-NAME, administered either acutely by intravenous route or chronically by the oral induced sustained hypertension [16,21,22]. But Chaswal et al [23] showed that the acute hypertension provoked by L-NAME administration may not involve the renin angiotensin system which is at least partially responsible for the chronic response. The fact that MECZ could reduce both the acute and chronic hypertension induced by L-NAME indicates that it may use another pathway.…”

Section: Discussionmentioning

confidence: 99%

Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

Nyadjeu

Nguelefack‐Mbuyo

Atsamo

et al. 2013

BMC Complement Altern Med

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BackgroundPrevious study showed that the aqueous extract of the stem bark of Cinnamomum zeylanicum possesses antihypertensive and vasodilatory properties. The present work investigates the acute and chronic antihypertensive effects of the methanol extract of Cinnamomum zeylanicum stem bark (MECZ) in L-NAME-induced hypertensive rats.MethodsThe acute antihypertensive effects of MECZ (5, 10 and 20 mg/kg) administered intravenously were evaluated in rats in which acute arterial hypertension has been induced by intravenous administration of L-NAME (20 mg/kg). For chronic antihypertensive effects, animals were treated with L-NAME (40 mg/kg/day) plus the vehicle or L-NAME (40 mg/kg/day) in combination with captopril (20 mg/kg/day) or MECZ (300 mg/kg/day) and compared with control group receiving only distilled water. All drugs were administered per os and at the end of the experiment that lasted for four consecutive weeks, blood pressure was measured by invasive method and blood samples were collected for the determination of the lipid profile. The heart and aorta were collected, weighed and used for both histological analysis and determination of NO tissue content.ResultsAcute intravenous administration of C. zeylanicum extract (5, 10 and 20 mg/kg) to L-NAME-induced hypertensive rats provoked a long-lasting decrease in blood pressure. Mean arterial blood pressure decreased by 12.5%, 26.6% and 30.6% at the doses of 5, 10 and 20 mg/kg, respectively. In chronic administration, MECZ and captopril significantly prevented the increase in blood pressure and organs’ weights, as well as tissue histological damages and were able to reverse the depletion in NO tissue’s concentration. The MECZ also significantly lower the plasma level of triglycerides (38.1%), total cholesterol (32.1%) and LDL-cholesterol (75.3%) while increasing that of HDL-cholesterol (58.4%) with a significant low atherogenic index (1.4 versus 5.3 for L-NAME group).ConclusionMECZ possesses antihypertensive and organ protective effects that may result from its ability to increase the production of the endogenous NO and/or to regulate dyslipidemia.

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Section: Discussionmentioning

confidence: 99%

Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

Nyadjeu

Nguelefack‐Mbuyo

Atsamo

et al. 2013

BMC Complement Altern Med

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“…A similar result was obtained by Nguelefack-Mbuyo et al . [6] and Afkir and co-workers[21] using another angiotensin-converting enzyme, enalapril. The exact mechanism of this effect is yet to be known.…”

Section: Discussionmentioning

confidence: 99%

Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats

et al. 2010

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Objective:The present study aims at evaluating the effects of methanol/methylene chloride extract of the stem bark of Mammea africana on the renal function of L-NAME treated rats.Material and Methods:Normotensive male Wistar rats were divided into five groups respectively treated with distilled water, L-NAME (40 mg/kg/day), L-NAME + L-arginine (100 mg/kg/day), L-NAME + captopril (20 mg/kg/day) or L-NAME + M. africana extract (200 mg/kg/day) for 30 days. Systolic blood pressure was measured before and at the end of treatment. Body weight was measured at the end of each week. Urine was collected 6 and 24 h after the first administration and further on day 15 and 30 of treatment for creatinine, sodium and potassium quantification, while plasma was collected at the end of treatment for the creatinine assay. ANOVA two way followed by Bonferonni or one way followed by Tukey were used for statistical analysis.Results:M. africana successfully prevented the rise in blood pressure and the acute natriuresis and diuresis induced by L-NAME. When given chronically, the extract produced a sustained antinatriuretic effect, a non-significant increase in urine excretion and reduced the glomerular hyperfiltration induced by L-NAME.Conclusions:The above results suggest that the methanol/methylene chloride extract of the stem bark of M. africana may protect kidney against renal dysfunction and further demonstrate that its antihypertensive effect does not depend on a diuretic or natriuretic activity.

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“…Groups C and D were pre- treated with KV and CL, respectively, for 7 consecutive days before treatment with L- NAME. L- NAME was administered at a dose of 40 mg/kg/day,[27] while KV and CL were given at a dose of 200 mg/kg/day. [1620] All drugs were given by oral gavages, 5 times in a week for a period of 3 weeks.…”

Section: Methodsmentioning

confidence: 99%

Sub-acute effect of N^G-nitro-l-arginine methyl-ester (L-NAME) on biochemical indices ins rats: Protective effects of Kolaviron and extract of Curcuma longa L

2012

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Background:Kolaviron (KV) (biflavonoid from Garcinia kola) and extract of Curcuma longa (CL) are frequently used in folk medicine for treatment of hypertension. One of their mechanisms of action is to enhance antioxidant properties in animals. NG- nitro- l- arginine methyl- ester (L- NAME) is L- arginine analogue, which by binding to Nitric Oxide Synthase (NOS) may induce hypertension partly due to increase in tissues oxidative stress.Objectives:To investigate the effect of L- NAME on some biochemical indices and the possible protective effect of KV or CL.Materials and Methods:Four groups consisting of 6 rats each were used. One group served as control, second group received L- NAME (40 mg/kg/day). Third and fourth groups were treated with KV and CL, respectively and also received L- NAME. KV and CL were given at a dose of 200 mg/kg/day.Results:L- NAME caused a significant (P <0.05) increase in the levels of serum urea, creatine kinase and alanine aminotransferase relative to controls. L- NAME treated rats had markedly decreased hepatic catalase (CAT), superoxide dismutase (SOD), glutathione- S- transferase (GST) and reduced glutathione (GSH) levels. Precisely, L- NAME decreased CAT, SOD, GST and GSH by 48, 52, 76 and 40%, respectively. L- NAME intoxication significantly decreased (P <0.05) renal GSH and SOD levels. Also, L- NAME caused a significant (P <0.05) induction of lipid peroxidation (LPO) in the animals. Administration of KV or CL with L- NAME caused significant (P <0.05) inhibition of LPO and augments tissue antioxidant indices.Conclusion:These results confirm the adverse effect of L- NAME on biochemical indices and, the ability of kolaviron or Curcuma longa to ameliorate the alterations.

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Arbutus unedo prevents cardiovascular and morphological alterations in L-NAME-induced hypertensive rats

Cited by 72 publications

References 37 publications

Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats

Sub-acute effect of N^G-nitro-l-arginine methyl-ester (L-NAME) on biochemical indices ins rats: Protective effects of Kolaviron and extract of Curcuma longa L

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Arbutus unedo prevents cardiovascular and morphological alterations in L-NAME-induced hypertensive rats

Cited by 72 publications

References 37 publications

Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

Acute and chronic antihypertensive effects of Cinnamomum zeylanicum stem bark methanol extract in L-NAME-induced hypertensive rats

Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats

Sub-acute effect of NG-nitro-l-arginine methyl-ester (L-NAME) on biochemical indices ins rats: Protective effects of Kolaviron and extract of Curcuma longa L

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Product

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Sub-acute effect of N^G-nitro-l-arginine methyl-ester (L-NAME) on biochemical indices ins rats: Protective effects of Kolaviron and extract of Curcuma longa L