Sub-acute effect of N^G-nitro-l-arginine methyl-ester (L-NAME) on biochemical indices ins rats: Protective effects of Kolaviron and extract of Curcuma longa L

Abstract

Background:Kolaviron (KV) (biflavonoid from Garcinia kola) and extract of Curcuma longa (CL) are frequently used in folk medicine for treatment of hypertension. One of their mechanisms of action is to enhance antioxidant properties in animals. NG- nitro- l- arginine methyl- ester (L- NAME) is L- arginine analogue, which by binding to Nitric Oxide Synthase (NOS) may induce hypertension partly due to increase in tissues oxidative stress.Objectives:To investigate the effect of L- NAME on some biochemical indices and the possible protective effect of KV or CL.Materials and Methods:Four groups consisting of 6 rats each were used. One group served as control, second group received L- NAME (40 mg/kg/day). Third and fourth groups were treated with KV and CL, respectively and also received L- NAME. KV and CL were given at a dose of 200 mg/kg/day.Results:L- NAME caused a significant (P <0.05) increase in the levels of serum urea, creatine kinase and alanine aminotransferase relative to controls. L- NAME treated rats had markedly decreased hepatic catalase (CAT), superoxide dismutase (SOD), glutathione- S- transferase (GST) and reduced glutathione (GSH) levels. Precisely, L- NAME decreased CAT, SOD, GST and GSH by 48, 52, 76 and 40%, respectively. L- NAME intoxication significantly decreased (P <0.05) renal GSH and SOD levels. Also, L- NAME caused a significant (P <0.05) induction of lipid peroxidation (LPO) in the animals. Administration of KV or CL with L- NAME caused significant (P <0.05) inhibition of LPO and augments tissue antioxidant indices.Conclusion:These results confirm the adverse effect of L- NAME on biochemical indices and, the ability of kolaviron or Curcuma longa to ameliorate the alterations.

Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats

“…In the heart and kidneys however, the concentration of NO in L-NAME groups was comparable to normotensive control. This finding was consistent with previous studies by Adaramoye et al [38] and Berkban et al [39] who also observed normal levels of NO in heart and kidney of L-NAME treated rats. Abdel-Raham et al [40] proposed that eNOS protein expression may be up-regulated in L-NAME treated rats as a counter-regulatory mechanism to compensate for increased BP.…”

Antihypertensive Effects of Roselle‐Olive Combination in L‐NAME‐Induced Hypertensive Rats

“…A strong body of evidences suggests that chronic inhibition of NOS by L-NAME alters various biochemical indices [ 59 ]. In the current study, the activities of the liver marker enzymes (ALT, AST, and γ -GT) were significantly elevated after L-NAME administration in comparison with normal controls, denoting hepatic injury ( Table 2 ).…”

Blood pressure lowering effect of scopoletin on oxidative stress-associated hypertensive rats

“…Several biochemical processes such as catalase activity and lipid peroxidation are also altered. These processes may serve as alternative pathway in the metabolism of nitric oxide [37,38]. Therefore, the restoration of antioxidant capacity due to the administration of scopoletin and tempol may induce a better blood pressure lowering effect in the oxidative stress-associated hypertensive model.…”