Arc discharge-mediated disassembly of viral particles in water

Lee, Eunjung; Lee, Wooseong; Kim, Minwoo; Choi, Eun Ha; Kim, Yun-Ji

doi:10.1016/j.watres.2016.06.052

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…To further investigate the antiviral action of HP, the morphology of MNV-1 in low titer was analyzed by transmission electron microscopic (TEM) before and after treatment with PBS (0 μg/mL) and HP (250 μg/mL). Before treatment, MNV-1 particles were spherical (Figure 5A) and the size ranged from 30 to 35 nm as described previously [20]. After treatment with HP (250 μg/mL), the size of the MNV-1 particles increased to 80–100 nm in diameter.…”

Section: Resultssupporting

confidence: 73%

Antiviral Effects of Houttuynia cordata Polysaccharide Extract on Murine Norovirus-1 (MNV-1)—A Human Norovirus Surrogate

et al. 2019

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Houttuynia cordata is an herbal plant rich in polysaccharides and with several pharmacological activities. Human noroviruses (HuNoVs) are the most common cause of foodborne viral gastroenteritis throughout the world. In this study, H. cordata polysaccharide (HP), with a molecular weight of ~43 kDa, was purified from H. cordata water extract (HWE). The polysaccharide HP was composed predominantly of galacturonic acid, galactose, glucose, and xylose in a molar ratio of 1.56:1.49:1.26:1.11. Methylation and NMR analyses revealed that HP was a pectin-like acidic polysaccharide mainly consisting of α-1,4-linked GalpA, β-1,4-linked Galp, β-1,4-linked Glcp, and β-1,4-linked Xylp residues. To evaluate the antiviral activity of H. cordata extracts, we compared the anti-norovirus potential of HP with HWE and ethanol extract (HEE) from H. cordata by plaque assay (plaque forming units (PFU)/mL) for murine norovirus-1 (MNV-1), a surrogate of HuNoVs. Viruses at high (8.09 log10 PFU/mL) or low (4.38 log10 PFU/mL) counts were mixed with 100, 250, and 500 μg/mL of HP, HWE or HEE and incubated for 30 min at room temperature. H. cordata polysaccharide (HP) was more effective than HEE in reducing MNV-1 plaque formation, but less effective than HWE. When MNV-1 was treated with 500 μg/mL HP, the infectivity of MNV-1 decreased to an undetectable level. The selectivity indexes of each sample were 1.95 for HEE, 5.74 for HP, and 16.14 for HWE. The results of decimal reduction time and transmission electron microscopic revealed that HP has anti-viral effects by deforming and inflating virus particles, thereby inhibiting the penetration of viruses in target cells. These findings suggest that HP might have potential as an antiviral agent in the treatment of viral diseases.

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Section: Resultssupporting

confidence: 73%

Antiviral Effects of Houttuynia cordata Polysaccharide Extract on Murine Norovirus-1 (MNV-1)—A Human Norovirus Surrogate

et al. 2019

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“…Novel disinfection technologies, e.g. pulsed or low-pressure UV systems or plasma treatments have shown to reduce NoV concentrations and infectivity of surrogates (Lee et al 2016; Barret et al 2016). Finally, logistical constraints limit the physical modification of some facilities; for example, UV disinfection unit installation at CSOs is only practicable at larger facilities where there is sufficient space for additional plant and infrastructure (DCWW 2015).…”

Section: Regulating Nov In Shellfish Waters and Shellfish: Current Prmentioning

confidence: 99%

Critical Review on the Public Health Impact of Norovirus Contamination in Shellfish and the Environment: A UK Perspective

et al. 2017

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We review the risk of norovirus (NoV) infection to the human population from consumption of contaminated shellfish. From a UK perspective, risk is apportioned for different vectors of NoV infection within the population. NoV spreads mainly by person-to-person contact or via unsanitary food handling. NoV also enters the coastal zone via wastewater discharges resulting in contamination of shellfish waters. Typically, NoV persists in the marine environment for several days, with its presence strongly linked to human population density, wastewater discharge rate, and efficacy of wastewater treatment. Shellfish bioaccumulate NoV and current post-harvest depuration is inefficient in its removal. While NoV can be inactivated by cooking (e.g. mussels), consumption of contaminated raw shellfish (e.g. oysters) represents a risk to human health. Consumption of contaminated food accounts for 3–11% of NoV cases in the UK (~74,000 cases/year), of which 16% are attributable to oyster consumption (11,800 cases/year). However, environmental and human factors influencing NoV infectivity remain poorly understood. Lack of standard methods for accurate quantification of infective and non-infective (damaged) NoV particles represent a major barrier, hampering identification of an appropriate lower NoV contamination limit for shellfish. Future management strategies may include shellfish quality assessment (at point of harvest or at point of supply) or harvesting controls. However, poor understanding of NoV inactivation in shellfish and the environment currently limits accurate apportionment and risk assessment for NoV and hence the identification of appropriate shellfish or environmental quality standards.

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“…A wide variety of plasma technologies has been studied in the context of microbial reduction in food 13 , 14 , environment 15 , 16 and wound healing 17 . Reactive oxygen species (ROS), reactive nitrogen species (RNS) and their reaction products such as ozone, hydrogen peroxide, nitrous acid and nitric acid generates by plasma 18 , 19 , which are soluble in water and are capable of biological sterilization 20 .…”

Section: Introductionmentioning

confidence: 99%

“…Reactive oxygen species (ROS), reactive nitrogen species (RNS) and their reaction products such as ozone, hydrogen peroxide, nitrous acid and nitric acid generates by plasma 18 , 19 , which are soluble in water and are capable of biological sterilization 20 . Our previous research showed the effectiveness of different types of plasma, including arc and dielectric barrier discharge (DBD), on viral and pathogenic inactivation in refs 14 – 16 , 20 – 22 . Plasma discharged water has the ability to reduce microbial contamination from the different types of food surfaces including onion, perilla leaf, lettuce etc., without changing the physiochemical properties of the treated sample 14 , 21 .…”

Section: Introductionmentioning

confidence: 99%

Roles of oxides of nitrogen on quality enhancement of soybean sprout during hydroponic production using plasma discharged water recycling technology

Lee

Khan

Shim

et al. 2018

Sci Rep

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This study was performed to assess the effect of plasma-discharged water recycling technology as irrigation water on soybean sprout production. Two different types of irrigation water were used individually for cultivation, including plasma discharged water as a source of oxides of nitrogen and tap water, irrigation water was recycled for every 30 minutes. Plasma discharged irrigation water reduced overall 4.3 log CFU/ml aerobic microbe and 7.0 log CFU/ml of artificially inoculated S. Typhimurium within 5 minutes and 2 minutes, respectively, therefore sprout production occurs in a hygienic environment. Using of plasma-discharged water for cultivation, increases the amount of ascorbate, asparagine, and γ-aminobutyric acid (GABA) significantly (p < 0.05), in the part of cotyledon and hypocotyl of soybean sprout during 1 to 4 days of farming. A NO scavenger, 2-(4-carboxy-phenyl)-4,4,5,5-tetramethylimidazoline-1-oxy-3-oxide (cPTIO), was added in irrigation water to elucidate the roles of the oxides of nitrogen such as NO3−, NO2− generated in plasma discharged water. It was observed that all three nutrients decreased in the cotyledon part, whereas ascorbate and GABA contents increased in the hypocotyl and radicle part of bean sprout for the same duration of farming. The addition of NO scavenger in the irrigation water also reduced growth and overall yield of the soybean sprouts. A recycling water system with plasma-discharged water helped to reduce the amount of water consumption and allowed soybean sprouts growth in a hygienic environment during the hydroponic production.

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Arc discharge-mediated disassembly of viral particles in water

Cited by 13 publications

References 26 publications

Antiviral Effects of Houttuynia cordata Polysaccharide Extract on Murine Norovirus-1 (MNV-1)—A Human Norovirus Surrogate

Antiviral Effects of Houttuynia cordata Polysaccharide Extract on Murine Norovirus-1 (MNV-1)—A Human Norovirus Surrogate

Critical Review on the Public Health Impact of Norovirus Contamination in Shellfish and the Environment: A UK Perspective

Roles of oxides of nitrogen on quality enhancement of soybean sprout during hydroponic production using plasma discharged water recycling technology

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