Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis

Abstract

<jats:p>A polyphasic taxonomic study was performed on an unidentified <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5813" xlink:type="simple">Arcanobacterium</jats:ext-link> </jats:named-content> </jats:italic>-like Gram-stain-positive bacterium designated strain C605018/01/1<jats:sup>T</jats:sup> isolated from a milk sample collected from the udder of a cow at <jats:italic>post mortem</jats:italic>. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5813" xlink:type="simple">Arcanobacterium</jats:ext-link> </jats:named-content> </jats:italic> and was most closely related to the type strain of <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5818" xlink:type="simple">Arcanobacterium pluranimalium</jats:ext-link> </jats:named-content> </jats:italic> (99.76 %); sequence similarities to all other <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5813" xlink:type="simple">Arcanobacterium</jats:ext-link> </jats:named-content> </jats:italic> species were below 97 %. The wet-lab DNA–DNA hybridization values among strain C605018/01/1<jats:sup>T</jats:sup> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5818" xlink:type="simple">A. pluranimalium</jats:ext-link> </jats:named-content> </jats:italic> DSM 13483ᵀ were low, 16.9 % (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01<jats:sup>T</jats:sup> displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H<jats:sub>4</jats:sub>) supported the affiliation of this strain to the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5813" xlink:type="simple">Arcanobacterium</jats:ext-link> </jats:named-content> </jats:italic>. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1<jats:sup>T</jats:sup> should be classified as representing a novel species, <jats:italic>Arcanbacterium bovis</jats:italic> sp. nov. The type strain C605018/01/1<jats:sup>T</jats:sup> (CCUG 45425<jats:sup>T</jats:sup>=DSM 107286<jats:sup>T</jats:sup>=BCCM/LMG 30783<jats:sup>T</jats:sup>)</jats:p>

Arcanobacterium buesumense sp. nov., isolated from an anal swab of a male harbour seal (Phoca vitulina)

“…The major components were C 16 : 0 (30.94%), C 18 : 1 ω9c (30.44%) and C 18 : 0 (18.29%), which was congruent with the fatty acid profiles of other species of the genus Arcanobacterium [2,4,7]. Fatty acid profiles published by Hijazin et al [4] and Sammra et al [7][8][9] were analysed in the same laboratory as the data represented here by the use of biomass grown under the same conditions and harvested at the same growth stage.…”

“…[4] and Sammra et al . [7–9] were analysed in the same laboratory as the data represented here by the use of biomass grown under the same conditions and harvested at the same growth stage.…”

“…These authors proposed that genus Arcanobacterium should be split into two genera, with Arcanobacterium haemolyticum , Arcanobacterium phocae , Arcanobacterium pluranimalium and Arcanobacterium hippocoleae belonging to the genus Arcanobacterium sensu stricto and Arcanobacterium abortisuis , Arcanobacterium bernardiae , Arcanobacterium bialowiezense , Arcanobacterium bonasi and Arcanobacterium pyogenes being transferred to he genus Trueperella . More recently, Arcanobacterium canis and Arcanobacterium phocisimile [3, 4], Arcanobacterium urinimassiliense [5], Arcanobacterium ihumii [6], Arcanobacterium pinnipediorum [7], Arcanobacterium wilhelmae and Arcanobacterium bovis [8, 9] were described.…”

Identification and Characterization of Arcanobacterium canis from Companion Animals in Germany and The United Kingdom

“…It indicates that the observed phenotypic characteristics of these isolates closely matched those previously documented for A. canis DSM 25104 T , a reference isolate [12] . These phenotypic characteristics are crucial in ensuring accurate species classification since each species of Arcanobacterium shows specific phenotype characteristics [18,26,31,32] . The results of this phenotypic research strongly indicated that both isolates were true of the A. canis species, as their features closely resembled those of a wellestablished reference isolate.…”

Arcanobacterium canis strain DSM 25104 isolated from an English bulldog suffering from otitis externa: complete genome sequence

“…Gram-positive, facultative anaerobic rods of the genus Arcanobacterium occur in both human and animal hosts. The latter includes domestic animals, such as dogs ( 1 , 2 ), cats ( 2 ), and horses ( 3 ), livestock, such as cattle ( 4 ) or sheep ( 5 ), and wildlife animals, such as seals, sea lions, and sea otters ( 6 ).…”