Archaea Symbiont of T. cruzi Infection May Explain Heart Failure in Chagas Disease

Higuchi, Maria de Lourdes; Kawakami, Joyce T.; Ikegami, Renata Nishiyama; Reis, Marlene Paulino dos; Pereira, Jaqueline de Jesus; Ianni, Bárbara Maria; Buck, Paula; Oliveira, Luanda Mara da Silva; Santos, Marília Harumi Higuchi dos; Hajjar, Ludhmila Abrahão; Bocchi, Edimar Alcides

doi:10.3389/fcimb.2018.00412

Cited by 8 publications

(8 citation statements)

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“…EVs are usually considered protective, promoting the removal of harmful cellular compounds and leading to cell survival, but have been described as deleterious, favoring the proliferation of cancer cells, delivering pathogens to uninfected cells and contributing to the development of heart failure (26,48). Studying Chagas disease, we observed that heart failure in chagasic patients was directly associated with an increased quantity of EVs containing DNA and archaeal collagenase in serum, strong evidence that collagenase-producing archaea play a role in the development of worse clinical outcomes in cardiovascular disease (14).…”

Section: Discussionmentioning

confidence: 68%

“…An atherosclerotic plaque containing proinflammatory cytokines, reactive oxygen species, high levels of oxidized low-density lipoproteins (oxLDL), cholesterol crystals and damage-associated molecular patterns (8), due to damaged proteins, lipids and DNA (9), is a microenvironment at risk for plaque rupture and thrombosis (10), and might be explained by the presence of a pathogenic microbial community (11). In previous work by our group, the presence of archaea was related to the aggravation of atherosclerosis (12,13) and with heart failure in Chagas disease, where archaeal DNA was detected in nanoparticles in the serum (14).…”

Section: Introductionmentioning

confidence: 98%

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Morphomolecular Characterization of Serum Nanovesicles From Microbiomes Differentiates Stable and Infarcted Atherosclerotic Patients

Moreno

Ramires

Lotufo

et al. 2021

Front. Cardiovasc. Med.

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Microbial communities are considered decisive for maintaining a healthy situation or for determining diseases. Acute myocardial infarction (AMI) is an important complication of atherosclerosis caused by the rupture of atheroma plaques containing proinflammatory cytokines, reactive oxygen species, oxidized low-density lipoproteins (oxLDL), damaged proteins, lipids, and DNA, a microenvironment compatible with a pathogenic microbial community. Previously, we found that archaeal DNA-positive infectious microvesicles (iMVs) were detected in vulnerable plaques and in the sera of Chagas disease patients with heart failure. Now, we characterize and quantify the levels of serum microbiome extracellular vesicles through their size and content using morphomolecular techniques to differentiate clinical outcomes in coronary artery disease (CAD). We detected increased numbers of large iMVs (0.8–1.34 nm) with highly negative surface charge that were positive for archaeal DNA, Mycoplasma pneumoniae antigens and MMP9 in the sera of severe AMI patients, strongly favoring our hypothesis that pathogenic archaea may play a role in the worst outcomes of atherosclerosis. The highest numbers of EVs <100 nm (exosomes) and MVs from 100 to 200 nm in the stable atherosclerotic and control healthy groups compared with the AMI groups were indicative that these EVs are protective, entrapping and degrading infectious antigens and active MMP9 and protect against the development of plaque rupture.Conclusion: A microbiome with pathogenic archaea is associated with high numbers of serum iMVs in AMI with the worst prognosis. This pioneering work demonstrates that the morphomolecular characterization and quantification of iEVs in serum may constitute a promising serum prognostic biomarker in CAD.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 98%

Morphomolecular Characterization of Serum Nanovesicles From Microbiomes Differentiates Stable and Infarcted Atherosclerotic Patients

Moreno

Ramires

Lotufo

et al. 2021

Front. Cardiovasc. Med.

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“…The prevalence rate among adults in China is as high as 0.9%, which accounts for 1% - 2% in developed countries, causing great harm. 13 , 14 Pulmonary infection and heart failure affect each other and are causal to each other. Specifically, patients with heart failure have poor immune function, which can affect pulmonary circulation and increase the risk of lung infections.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic characteristics of pulmonary infection in hospitalized patients with chronic heart failure and diagnostic value of sTREM-1, sCD163, and sTWEAK

Zheng¹

2022

Pak J Med Sci

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Objective: To investigate the pathogenic characteristics of pulmonary infection in hospitalized patients with chronic heart failure as well as the diagnostic value of soluble myeloid cell expression triggering receptor-1 (sTREM-1), soluble CD163 (sCD163) and soluble tumor necrosis factor-like weak inducing factor (sTWEAK). Methods: A total of 72 patients with pulmonary infection who were hospitalized with chronic heart failure from December 2017 to December 2019 in the Department of Cardiology of Hebei Baoding Huaying Hospital of Traditional Chinese Medicine, China, were selected as the infection group, seventy-two patients without pulmonary infection who were hospitalized with chronic heart failure were selected as the non-infection group, and 50 healthy subjects who underwent physical examination in the hospital during the same period were selected as the control group. The distribution characteristics of pathogens in the infection group were statistically analyzed. The levels of sTREM-1, S CD163 and STweak in serum of patients with different infection severity and different cardiac function grades were compared among the three groups. Receiver operating characteristic curve (ROC) was utilized to evaluate the predictive value of the three indicators for the adverse prognosis of patients in hospital. Results: A total of 76 strains of pathogens were cultured from two hospitalized patients with pulmonary infection of chronic heart failure, among which 43 strains (56.58%) were gram-negative bacteria, 29 strains (38.15%) were gram-positive bacteria, and four strains (5.26%) were fungi. The levels of sTREM-1 and sCD163 in the control group, non-infection group and infection group were gradually increased (p<0.05), while there was no difference in sTWEAK between the infection group and the non-infection group (p>0.05). In the infection group, the expression levels of sTREM-1 and sCD163 increased with the severity of infection, with statistically significant differences (p<0.05), while there was no statistically significant difference in the expression level of sTWEAK among different infection severity (p>0.05). The higher the cardiac function grade of patients in the infection group, the higher the levels of sTREM-1 and sCD163, and the lower the level of sTWEAK, with a statistical significance (p<0.05). ROC analysis results showed that the serum sTREM-1, sCD163, and sTWEAK levels for the poor prognosis of patients with CHF combined with lung infection had areas under the curve of 0.864, 0.870, and 0.822, respectively, and the 95% CI values were 0.787-0.941, 0.795-0.945 and 0.733-0.910, respectively, all p<0.001. Conclusions: Pulmonary infection in hospitalized patients with chronic heart failure is mainly caused by gram-negative bacteria. Detection of sTREM-1, sCD163, and sTWEAK levels is of certain value in judging the condition and prognosis, which is worthy of clinical promotion. doi: https://doi.org/10.12669/pjms.38.3.4758 How to cite this:Zheng F. Pathogenic characteristics of pulmonary infection in hospitalized patients with chronic heart failure and diagnostic value of sTREM-1, sCD163, and sTWEAK. Pak J Med Sci. 2022;38(3):---------. doi: https://doi.org/10.12669/pjms.38.3.4758 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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“…Overall, this microRNA's potential as a non-invasive biomarker for the early detection of CD requires further exploration in human cohorts. A study that isolated EVs from patients with different clinical presentations of CD reported that EVs isolated from patient serum contained archaeal DNA and increased collagenase activity, which led to heart dilatation [39]. High expression of anti-archaemetzincin-1 (AMZ1) antigens in EVs, on the other hand, has been associated with a protective role in asymptomatic patients, potentially preventing the development of heart failure.…”

Section: Do Extracellular Vesicles Have Biomarker Utility?mentioning

confidence: 99%

Extracellular Vesicles: Translational Agenda Questions for Three Protozoan Parasites

Tandoh,

Ibarra‐Meneses,

Langlais

et al. 2024

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The protozoan parasites Plasmodium falciparum, Leishmania spp. and Trypanosoma cruzi continue to exert a significant toll on the disease landscape of the human population in sub‐Saharan Africa and Latin America. Control measures have helped reduce the burden of their respective diseases—malaria, leishmaniasis and Chagas disease—in endemic regions. However, the need for new drugs, innovative vaccination strategies and molecular markers of disease severity and outcomes has emerged because of developing antimicrobial drug resistance, comparatively inadequate or absent vaccines, and a lack of trustworthy markers of morbid outcomes. Extracellular vesicles (EVs) have been widely reported to play a role in the biology and pathogenicity of P. falciparum, Leishmania spp. and T. cruzi ever since they were discovered. EVs are secreted by a yet to be fully understood mechanism in protozoans into the extracellular milieu and carry a cargo of diverse molecules that reflect the originator cell's metabolic state. Although our understanding of the biogenesis and function of EVs continues to deepen, the question of how EVs in P. falciparum, Leishmania spp. and T. cruzi can serve as targets for a translational agenda into clinical and public health interventions is yet to be fully explored. Here, as a consortium of protozoan researchers, we outline a plan for future researchers and pose three questions to direct an EV's translational agenda in P. falciparum, Leishmania spp. and T. cruzi. We opine that in the long term, executing this blueprint will help bridge the current unmet needs of these medically important protozoan diseases in sub‐Saharan Africa and Latin America.

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Archaea Symbiont of T. cruzi Infection May Explain Heart Failure in Chagas Disease

Cited by 8 publications

References 50 publications

Morphomolecular Characterization of Serum Nanovesicles From Microbiomes Differentiates Stable and Infarcted Atherosclerotic Patients

Morphomolecular Characterization of Serum Nanovesicles From Microbiomes Differentiates Stable and Infarcted Atherosclerotic Patients

Pathogenic characteristics of pulmonary infection in hospitalized patients with chronic heart failure and diagnostic value of sTREM-1, sCD163, and sTWEAK

Extracellular Vesicles: Translational Agenda Questions for Three Protozoan Parasites

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