Architecture of Condensed DNA in the Nucleoid of Escherichia coli Bacterium

Krupyanskiǐ, Yu. F.; Kovalenko, Vladislav; Лойко, Н. Г.; Generalova, A. A.; Moiseenko, Andrey; Терешкин, Э. В.; Sokolova, Olga; Терешкина, К. Б.; Эль-Регистан, Г. И.; Popov, A.

doi:10.1134/s0006350922040133

Cited by 4 publications

(1 citation statement)

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“…Only small changes in energy within ±5% are observed. DNA inside the crystal adapts to the internal environment and adjusts to the shape of the channels, which is consistent with previous results [ 63 ]. The terminal regions of DNA lying outside the channels, as well as free DNAs in solution, do not undergo significant conformational changes ( Figure 6 ).…”

Section: Resultssupporting

confidence: 92%

DNA-Binding Protein Dps Protects Escherichia coli Cells against Multiple Stresses during Desiccation

Лойко

Терешкина

Kovalenko

et al. 2023

Biology

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Gradual dehydration is one of the frequent lethal yet poorly understood stresses that bacterial cells constantly face in the environment when their micro ecotopes dry out, as well as in industrial processes. Bacteria successfully survive extreme desiccation through complex rearrangements at the structural, physiological, and molecular levels, in which proteins are involved. The DNA-binding protein Dps has previously been shown to protect bacterial cells from many adverse effects. In our work, using engineered genetic models of E. coli to produce bacterial cells with overproduction of Dps protein, the protective function of Dps protein under multiple desiccation stresses was demonstrated for the first time. It was shown that the titer of viable cells after rehydration in the experimental variants with Dps protein overexpression was 1.5–8.5 times higher. Scanning electron microscopy was used to show a change in cell morphology upon rehydration. It was also proved that immobilization in the extracellular matrix, which is greater when the Dps protein is overexpressed, helps the cells survive. Transmission electron microscopy revealed disruption of the crystal structure of DNA–Dps crystals in E. coli cells that underwent desiccation stress and subsequent watering. Coarse-grained molecular dynamics simulations showed the protective function of Dps in DNA–Dps co-crystals during desiccation. The data obtained are important for improving biotechnological processes in which bacterial cells undergo desiccation.

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Section: Resultssupporting

confidence: 92%