The Sisson Brook W-Mo-Cu deposit was formed by hydrothermal fluids likely related to the Nashwaak Granites (muscovite-biotite granite, Group I; and biotite granite, Group II) and related dykes (biotite granitic dykes, Group III, and a feldspar-biotitequartz porphyry dyke, Group IV). Chemical data obtained using EPMA and LA-ICP-MS data of primary magmatic biotites were used to investigate magmatic processes and associated hydrothermal fluids. Trace element features of biotite in Group I two-mica granite suggest more magmatic processes along with a simple fractional crystallization. The K/Rb ratios and compatible elements (Cr, Ti, Co, V, and Ba) in biotite from Groups II, III, and IV decreases, whereas incompatible elements including Ta, Tl, Ga, Cs, Li, and Sn increase with magma fractionation. No correlation of Cu, W and Mo with K/Rb ratios is evident, Corresponding author.