Arcobacter roscoffensis sp. nov., a marine bacterium isolated from coastal seawater

Abstract

A novel Gram-negative, aerobic, motile, rod-shaped, beige-pigmented bacterium, strain ARW1-2F2T, was isolated from a seawater sample collected from Roscoff, France. Strain ARW1-2F2T was catalase-negative and oxidase-positive, and grew under mesophilic, neutrophilic and halophilic conditions. The 16S rRNA sequences revealed that strain ARW1-2F2T was closely related to Arcobacter lekithochrous LFT 1.7T and Arcobacter caeni RW17-10T(95.8 and 95.5 % gene sequence similarity, respectively). The genome of strain ARW1-2F2T was sequenced and had a G+C content of 28.7%. Two different measures of genome similarity, average nucleotide identity based on blast and digital DNA–DNA hybridization, indicated that strain ARW1-2F2T represents a new Arcobacter species. The predominant fatty acids were C16 : 1 ω7c/C16 : 1 ω6c and C18 : 1 ω7c/C18 : 1 ω6c. The results of a polyphasic analysis supported the description of strain ARW1-2F2T as representing a novel species of the genus Arcobacter , for which the name Arcobacter roscoffensis sp. nov. is proposed with the type strain ARW1-2F2T (DSM 29169T=KCTC 52423T).

Genomic diversity and taxonomic marker for Arcobacter species

“…Arcobacter was generally described as possessing differentiated abilities from Campylobacter , namely the ability to grow in aerobic conditions and at temperatures between 15 and 30°C ( Vandamme et al, 1992 ); however, this principle has been changed by the increased number of new species. Nowadays, Arcobacter species inhabit a wide range of ecological niches, encompassing diverse environments such as marine environments, wastewater and drinking water systems, animal feces, plants, and even oil fields, among others ( Van Driessche et al, 2005 ; Collado and Figueras, 2011 ; Rathlavath et al, 2017 ; On et al, 2021 ; Pascual et al, 2023 ). Some Arcobacter species have been detected in or isolated from the stools of patients with and without diarrhea, occasionally being associated with conditions such as bacteremia, endocarditis, and peritonitis ( Vandenberg et al, 2004 ; Ho et al, 2006 ; Van den Abeele et al, 2014 ; Isidro et al, 2020 ).…”

“…Furthermore, it is crucial to acknowledge that the actual prevalence of Arcobacter species may be underestimated due to the constraints imposed by current detection and identification methods ( Hanel et al, 2016 ). Currently, the Arcobacter genus consists of 34 species with validly published and accurately designated names 1 ( Pascual et al, 2023 ). In previous studies, the similarity of the 16S rRNA gene was considered a decisive characteristic for a taxonomic assignation at the genus level ( Roth et al, 2003 ; Clarridge, 2004 ).…”