Are blood group isoantigens lost from malignant prostatic epithelium? Immunohistochemical support for the preservation of the H isoantigen

Abstract

Summary Previous studies while demonstrating the presence of blood group isoantigens on normal prostatic epithelium have failed to identify such antigens on malignant prostatic tissue. Using a series of blood group specific monoclonal antibodies directed towards the A, B, H and Y antigens we have reinvestigated blood group isoantigen expression in both benign prostatic hypertrophy and prostatic adenocarcinoma. Results obtained from areas of benign prostatic hypertrophy are in broad agreement with those published however though we were unable to detect either A or B blood group isoantigens Type 2H and Y isoantigens were identified in 10 of the 12 tumours. These findings, while differing from previously reported results, lend support to the suggested connection between ontogenesis, oncogenesis and blood group isoantigen expression and also support the proposed link between Type 2 structures and malignant transformation.

The expression of ABH and Y blood group antigens in benign and malignant breast tissue: The preservation of the H and Y antigens in malignant epithelium

“…Mounted slides were viewed under an Olympus CH microscope and photographed onto KB14 film (ASA 20). The controls included in this study were identical to those we documented in our report on BGI expression with the prostate (Vowden et al, 1986).…”

“…Results reported here and in our concurrent studies on the prostate (Vowden et al, 1986) have only served to emphasize the marked cell to cell variation in BGI expression that occurs. This may represent changes in antigen expression by cells at different stages of the cell cycle.…”

“…Of the earlier studies two have failed to identify BGIs in any breast malignancy including in situ carcinomas (Strauchen et al, 1980;Shull et al, 1981 (Vowden et al, 1986) Kim & Isaacs (1975). Springer et al (1975Springer et al ( , 1979 (Alroy et al, 1978;Wolk & Bishop, 1983).…”

Integration of the Transcriptome and Glycome for Identification of Glycan Cell Signatures

“…In terms of blood group structures which include antigens A, B, Le a , Le b, Le y , Le x and H in Figure 4, the H(II) and the Ley epitopes, containing α1,2-fucose linkages, are predicted in these types of prostate cancer cells, with greater abundance in high passage LNCaP cells (Figure 5). Indeed, these epitopes have been reported as characteristic markers for prostate cancer [7], [10], [26], [27], [28], [29], [30].…”

A new monoclonal antibody (A46-B/B10) highly specific for the blood group H type 2 epitope: Generation, epitope analysis, serological and histological evaluation

“…The H type 2 n li r I -_-antigen recognized by mab A46-B/B10 is a blood group precursor and can therefore be considered a carcinomaassociated antigen of tissues belonging to the first category. It has been reported to accumulate in mammary carcinomas (Vowden et al, 1986b), squamous cell carcinomas (Kimmel et al, 1986), prostate carcinomas (Vowden et al, 1986a), and precancerous oral epithelium (Dabelsteen et al, 1983). In such studies the use of strictly specific and thoroughly tested mabs is essential.…”