Are fluorescent bodies of Y-spermatozoa detectable in common with mammalan species?

Ogawa, Shyoso; Yamakawa, Hirohito; Yamanoi, Junko; Nishida, S.; Kano, Yasuhiko; Takeshima, Tsutomu; Tauchi, Kiyonori; Nagashima, Hiroshi

doi:10.1016/s0093-691x(88)80032-3

Cited by 18 publications

(11 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tabela 2 -Médias obtidas na análise de variância para a identificação dos espermatozoides portadores do cromossomo Y nos gradientes de densidades testados Ogawa et al (1988), uma vez que é um método simples e rápido e permite o processamento e análise de todos os grupos experimentais e repetições no mesmo dia do experimento. Apesar de Ishijima et al (1991) terem observado em seus experimentos que a coloração com quinacrina mustarda deu um erro de 16,4%, Blottner et al (1994) não observaram o mesmo quando compararam os resultados de seu experimento com aquele obtido por hibridização in situ.…”

Section: Resultsunclassified

See 1 more Smart Citation

Sexagem de espermatozoides bovinos por centrifugação em gradiente descontínuo de densidade de Percoll

Lima¹,

Moreira‐Filho

Lucio

et al. 2011

R. Bras. Zootec.

View full text Add to dashboard Cite

RESUMO -O objetivo neste trabalho foi desenvolver um método de seleção do sexo de espermatozoides bovinos por centrifugação em gradiente de densidade de Percoll. Utilizou-se sêmen congelado de touros mantidos em regime de colheita de sêmen. A fração de espermatozoides X ou Y foi separada por centrifugação em treze diferentes gradientes de densidade de Percoll formados por 1 a 12 camadas com densidades que variaram de 1,004 g/mL a 1,123 g/mL. As soluções com diferentes densidades foram preparadas misturando-se, em proporções diferentes, meio de cultura Hank's e uma solução estoque composta de NaCl 1,5 M e Percoll (1:9, v/v). Sobre cada gradiente foi colocado um total de 50 × 10 6 espermatozoides descongelados em 0,7 mL de meio Hank's e centrifugados a 250 X g por 30 minutos, em rotor horizontal, a 25°C. Os espermatozoides das frações superior e inferior foram tratados com Quinacrina Mustarda e analisados (200 deles) quanto à presença do corpúsculo-F. Dos espermatozoides encontrados no sedimento de dois gradientes, compostos de 8 e 12 frações com densidades variando entre 1,050 a 1,120 g/mL e 1,044 a 1,123 g/mL, respectivamente, visualizaram-se 25% com corpúsculo-F e os 75% restantes prováveis portadores do cromossomo X. O aumento na porcentagem de espermatozoides X após a centrifugação em gradiente de densidade permitirá que esse método de sexagem seja usado em larga escala na produção comercial de carne e leite bem como no teste de progênie. Palavras-chave: Bos taurus, seleção do sexo, sêmenSex selection in bovine spermatozoa by using Percoll discontinuos density gradient centrifugation ABSTRACT -The objective of this work was to develop a bovine spermatozoid sex selection method by using Percoll density gradient centrifugation. It was used frozen semen of bulls kept in semen collection regime. Fraction X or Y was separated by centrifugation in three different Percoll density gradient formed by 1 to 12 layers with densities varying from 1.004 g/mL to 1.123 g/mL. Solutions with different densities were prepared by mixing, at different proportions, Hank's culture medium and a stock solution composed of NaCl 1.5 M and Percoll (1:9, v/v). On each gradient, it was put 50 × 10 6 spermatozoids thawed in Hank's medium and centrifuged at 250 X g for 30 min in a horizontal rotor, at 25°C. The spermatozoids in the superior and inferior fractions were treated with Mustard Quinacrine and analyzed (200 spermatozoids) for the presence of F-body. Of the spermatozoids found in the sediment of the two gradients, composed of 8 and 12fractions with densities varying from 1.050 to 1.120 g/mL and 1.044 to 1.123 g/mL, respectively, it was identified 25%with the F-body and the other 75% were probably X chromosome-bearing spermatozoids. Increase in percentage of the X-spermatozoid after density gradient centrifugation will allow this system of spermatozoid sexing to be used in large scale in commercial production of meat and milk as well as for progeny tests in bovines. IntroduçãoA sexagem de espermatozoides tem valor econômico signifi...

show abstract

Section: Resultsunclassified

“…Foi usada uma lâmpada de mercúrio de alta pressão (VSH-200 MB) como fonte de luz e lentes objetivas de plano S 40x. Duzentas células foram analisadas de cada amostra e foi calculada a porcentagem de corpúsculos-F (Ogawa et al, 1988).…”

Section: Methodsunclassified

Sexagem de espermatozoides bovinos por centrifugação em gradiente descontínuo de densidade de Percoll

Lima¹,

Moreira‐Filho

Lucio

et al. 2011

R. Bras. Zootec.

View full text Add to dashboard Cite

show abstract

“…The availability of a cheap, rapid and simple method of distinguishing between X and Y chromosome-bearing sperm, allowing ready assessment of the efficacy of separation techniques and elucidation of conditions for their optimal performance, may well have allowed greater progress in the separation of human X and Y sperm than that of livestock. It remains to be seen whether the recent report of successful F-body staining in a number of mammalian species (Ogawa et al 1988) results in its widespread use, or whether such methods will be superseded by less equivocal techniques based on sex chromosome specific probes. FCM analysis has also found a role in evaluating the success of such separation attempts (see Table 2) but the expense and technological sophistication of this technique currently confine it to a few laboratories.…”

Section: Discussionmentioning

confidence: 99%

“…Reports of successful F-body staining of bull sperm (termed B-bodies) after treatment with proteases (Bhattacharya 1975;Bhattacharya et al 1977) have remained largely unconfirmed. Despite great interest in such technology there have been few successes, the reported occurrence of F-bodies in the sperm of several mammalian species varying between 5 and 40% (Ogawa et al 1988). Our attempts to detect F-bodies in ram sperm following protease digestion or detergent treatment have also been unsuccessful.…”

mentioning

confidence: 99%

Sex predetermination by separation of X and Y chromosome-bearing sperm: a review

Windsor

Evans²,

White³

1993

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Historical BackgroundCurrent interest in the possibility of predetermining the sex of human or animal progeny is only a recent manifestation of an age-old phenomenon. The antiquity of the subject is demonstrated by Talmudic references to coital methods of sex preselection, although their interpretation remains obscure (Rosner 1979).The possibility of choosing the sex of a child was also considered by fifth century Greeks. Despite their belief that supernatural forces designated fetal sex, selective infanticide was used to dispose of children with a variety of undesirable characteristics, including inappropriate sex [Cyrus the Great (Heredotus, Book I) and Oedipus]. As an alternative, parents were given access to a number of pre-natal sex selection measures (reviewed by Levin 1987).Hippocrates, for example, proposed that males developed more readily in the right side of the uterus, and females in the left. Appropriate sexual practices were believed to ensure deposition of semen in the correct portion of the female tract, followed (at least in theory) by development of a fetus of the desired sex. Alternatively, ligation or, in extreme cases, removal of the right testis (believed to produce males) or left testis would result in dispatch of the correct type of sperm. Other techniques relied upon manipulation of such factors as the geographical alignment of the nuptial bed, or the removal or retention of boots prior to sexual activity. Even Aristotle, whose belief in semen as the controlling factor in fetal development approached the absolute, attributed sex determination to the relative activity of the parents during intercourse. The popularity of such folkloric methods was maintained into the 20th century, by which time a variety of dietary, sexual and surgical techniques was also included.Such practices were based less upon empirical evidence than on the assumption that sex was determined 'not by inheritance but by the combined effect of external conditions' (Wilson 1896, cited by Farley 1982. This axiom was challenged by the observation that Mendelian genetics provided a possible mechanism for the inheritance of sex at the time of fertilization and the eventual realization that sex was determined by the chromosomal complement of the fertilizing sperm (reviewed by Farley 1982). Recognition of the fact that (in mammals) fertilization by X chromosome-bearing sperm produces females, and Y sperm males, has resulted in most of the scientific 20th century attempts at preconceptual sex selection involving alteration of the X: Y ratio in a sperm population, either in vitro prior to artificial insemination, or in the female tract.Impetus for sexing human sperm was provided by the desire to reduce the incidence of sex-linked genetic disorders. Improvements in artificial breeding technology and the demand for more efficient agricultural production has, however, led to increasing interest in predetermining the sex of the offspring of domestic livestock. The separation of X and Y

show abstract

“…It was tried to evaluate sperm separation by F-body staining (Ogawa et al, 1988). It was tried to evaluate sperm separation by F-body staining (Ogawa et al, 1988).…”

Section: Evaluation Of Sperm Separationmentioning

confidence: 99%

Effect of sperm motility on separation of bovine X- and Y-bearing spermatozoa by means of free-flow electrophoresis

Manger¹,

Bostedt²,

Schill

et al. 2009

Andrologia

View full text Add to dashboard Cite

Free-flow electrophoresis was used on bovine spermatozoa to test the hypothesis that there are surface charge differences between X and Y chromosome-bearing spermatozoa.Spermatozoa were deflected towards the anode by means of tromethamine (THAM) buffers of varying concentrations, and were separated into two populations under specific conditions. Experimental temperature and initial sperm motility had a significant effect on sperm distribution in the electric field. The results of DNA hybridization assays indicated an enrichment for Y chromosome-bearing spermatozoa.

show abstract

Are fluorescent bodies of Y-spermatozoa detectable in common with mammalan species?

Cited by 18 publications

References 4 publications

Sexagem de espermatozoides bovinos por centrifugação em gradiente descontínuo de densidade de Percoll

Sexagem de espermatozoides bovinos por centrifugação em gradiente descontínuo de densidade de Percoll

Sex predetermination by separation of X and Y chromosome-bearing sperm: a review

Effect of sperm motility on separation of bovine X- and Y-bearing spermatozoa by means of free-flow electrophoresis

Contact Info

Product

Resources

About