Are members of the Anopheles fluviatilis complex conspecific?

Singh, Om Prakash; Sindhania, Ankita; Sharma, Gunjan; Mishra, Shobhna; Sharma, Surya K.; Singh, Pargat; Das, Manoj

doi:10.1016/j.actatropica.2021.106149

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…However, the ITS2 sequence of An . stephensi from Saudi Arabia [ 37 ] is no different from Indian, Sri Lankan and African populations [ 31 , 39 , 40 ], exhibiting mito-nuclear discordance [ 41 ]. Though ITS2 has been widely and successfully used for discrimination of species, in some cases, for example in the case of Hyrcanus Complex, failed to differentiate An .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

et al. 2022

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Background Anopheles stephensi, an invasive malaria vector, has been reported to have three biological forms identifiable mainly based on the number of ridges present on the egg’s floats. Recently, the first intron of the odorant-binding protein-1 (AsteObp1) has been introduced as a molecular marker for the identification of these forms, and based on this marker, the presence of three putative sibling species (designated as species A, B and C) has been proposed. However, there is no data on the association of proposed markers with biological form or putative species on field populations. Methods Field collected and laboratory-reared An. stephensi were characterized for biological forms based on the number of ridges on the egg’s float. DNA sequencing of the partial AsteObp1 gene of An. stephensi individuals were performed by Sanger’s method, either directly or after cloning with a plasmid vector. Additionally, AsteObp1 sequences of various laboratory lines of An. stephensi were retrieved from a public sequence database. Results AsteObp1 intron-1 in Indian An. stephensi populations are highly polymorphic with the presence of more than 13 haplotypes exhibiting nucleotides as well as length-polymorphism (90-to-121 bp). No specific haplotype or a group of closely related haplotypes of intron-1 was found associated with any biological form identified morphologically. High heterozygosity for this marker with a low inbreeding coefficient in field and laboratory populations indicates that this marker is not suitable for the delimitation of putative sibling species, at least in Indian populations. Conclusions AsteObp1 cannot serve as a marker for identifying biological forms of An. stephensi or putative sibling species in Indian populations.

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Section: Discussionmentioning

confidence: 99%

Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

et al. 2022

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“…However, the ITS2 sequence of An. stephensi from Saudi Arabia (Munawar et al, 2020) is no different from Indian, Sri Lankan and African populations (Mishra et al, 2021, Surendran et al, 2018; Tadesse et al, 2019), exhibiting mito-nuclear discordance (Singh et al, 2021). Recent data on field populations of An.…”

Section: Discussionmentioning

confidence: 99%

“…However, the ITS2 sequence of An. stephensi from Saudi Arabia [37] is no different from Indian, Sri Lankan and African populations [31,[39][40], exhibiting mito-nuclear discordance [41]. Though ITS2 has been widely and successfully used for discrimination of species, in some cases, for example in the case of Hyrcanus Complex, failed to differentiate An.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

Singh

Mishra

Sindhania

et al. 2021

Preprint

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Background: Anopheles stephensi, an invasive malaria vector, has been reported to have three biological forms identifiable based on the number of ridges present on the egg floats and the dimension of eggs. Recently, these forms have been designated as sibling species based on the fixed differences in the DNA sequence of the first intron of the odorant-binding protein-1 (AsteObp1). In this study, we evaluated the utility of this neutral marker in designating sibling species or identifying biological forms. Methods: Field collected and laboratory-reared An. stephensi were characterized for biological forms based on the number of floats on egg-ridge. DNA sequencing of the partial AsteObp1 gene of An. stephensi individuals were performed by Sanger method, either directly or after cloning with a plasmid vector. Results: AsteObp1 intron-1 in Indian An. stephensi populations are highly polymorphic with the presence of more than 12 haplotypes exhibiting nucleotide- as well as length-polymorphism (90-to-121 bp). A majority of the field samples were heterozygous (up to 89% in the field populations). The phasing of haplotypes in heterozygotes through Sanger sequencing was challenging due to indels (1-to-24 bp) at multiple loci. No specific haplotype or monophyletic clade of intron-1 was found associated with a specific biological form. The inbreeding coefficient for this marker was close to zero in field and laboratory populations which refute the existence of sibling species based on the AsteObp1 marker. Conclusions: AsteObp1 cannot serve as a marker for the identification of biological forms of An. stephensi. The probable existence of sibling species in An. stephensi based on the AsteObp1 intron-1 is refuted.

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Molecular identification of Wolbachia strains infecting An. stephensi in the southern Iranian province of Fars

Shahriari-Namadi

hosseinizadeh

Izadpanah

et al. 2023

Int J Trop Insect Sci

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Are members of the Anopheles fluviatilis complex conspecific?

Cited by 3 publications

References 36 publications

Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species

Molecular identification of Wolbachia strains infecting An. stephensi in the southern Iranian province of Fars

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