Oasis agriculture in arid areas faces the constraints of scarce resources and a fragile ecological environment. Improving agricultural production efficiency is the key solution. However, there are few studies analyzing the relationship between farmers’ production efficiency and planting scale from a micro-empirical perspective. Herein, we study the seed-producing corn growers in Zhangye city, and supplement special survey data with national input–output survey data. We use data envelopment analysis to measure agricultural production efficiency, and tobit regression to calculate the marginal effects of factors affecting production efficiency on farms of different scales. The results show that production efficiency is greater for large-scale farmers than for small-scale farmers. Duration of technical training, education time of laborers, planting income, and productive expenditure are significantly positively correlated with production efficiency. Average age of farmers and the amount of pesticide and fertilizer use are significantly negatively correlated with production efficiency. Off-farm activities improve the production efficiency of small-scale farmers but inhibit it for medium- and large-scale farmers. Differences exist in marginal impacts for different scales of farmland. We conclude that expanding the scale of family farms and optimizing human capital are effective for improving agricultural production efficiency in arid oasis areas.