Athletes born closer to an arbitrary cut-off date are more likely to reach an elite level in sport, which is supported by a phenomenon called the relative age effect (RAE). It is important to determine whether this phenomenon is present in a sport to minimize this selection bias. This study aimed to investigate the prevalence of RAE in elite volleyball athletes, considering the influence of gender, the playing position (Setter, Middle, Libero, Opposite, and Outside Hitter) and the performance level (attack points, aces, and block points). The sample comprised 203 male and 193 female athletes competing in the Superliga A in the 2020/2021 season, which was equivalent to all of the teams of the championship. The data collection was performed during May and June, 2021. Athletes were organized according to gender, the playing position, and performance in the Superliga. For performance variables, athletes were separated based on the median value (90.0), and classified as high- or low-performance. Chi-squared tests were performed to verify differences between birth date distributions in relation to the aforementioned variables. Results indicated overrepresentation of relatively older male athletes (Q1 = 35.96%; Q2 = 27.59%; Q3 = 19.21%; Q4 = 17.24%), especially in Middles, Opposites, and Outside Hitters, regardless of their performance level. Considering females, no differences were found. Our findings suggest that RAE operates differently for men and women in elite Brazilian volleyball. The characteristics of the games played by male and female elite athletes may lead to different talent selection processes, affecting the likelihood of RAE prevalence.