Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Takahashi, Hiromi; Hirata, Roberto; Otsuki, Junko; Habara, Toshihiro; Hayashi, Naoaki

doi:10.1002/rmb2.12462

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…There was a preliminary study revealed not all morphologically 3PN embryos are genetically abnormal [26]. Some 3PN zygotes, with two normal-sized PNs and an additional smaller PN (2.1PN) embryos were diploid, however, predominantly aneuploid, and therefore could not be used for embryo transfer [27]. The de ciency of this study was that lack of analyzing the 3PN blastocysts genetic testing.…”

Section: Discussionmentioning

confidence: 96%

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Jiang

Yuan

et al. 2023

Preprint

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Aims To explore the influence of 3PN on outcomes in preimplantation genetic testing (PGT) cycles. Methods This was a retrospective study of patients undergoing PGT treatment included 139 PGT for aneuploidy (PGT-A) cycles and 285 cycles PGT for monogenic/single gene defects and/or chromosomal structural rearrangements (PGT-M/SR). The 424 PGT cycles were divided into two groups as follows: group 1 included 343 cycles with no 3PN zygotes and group 2 included 81 cycles with 3PN zygotes. 3PN cycle rate was compared between PGT-A and PGT-M/SR cycles. The outcomes of PGT were analyzed between the two groups. Results The female and male's age were significantly higher in PGT-A than PGT-M/SR cycles. Whereas 3PN rate was not significantly different between the two groups. The number of retrieved oocytes was significantly higher in 3PN group than Non 3PN group (13.98 ± 7.10 vs. 10.89 ± 6.65; P < 0.05). The MII oocyte rate, 2PN fertilizaion rate, D3 high embryo rate, biopsy blastocyst rate, per oocyte utilization and D5 blastocyst rate were significantly lower in 3PN group than Non 3PN group (P < 0.05). The results of chromosomal mosaic rate was significantly higher in 3PN group than Non 3PN group (16.3 vs. 11.9; P < 0.05). Conclusions No correlation was observed between 3PN formation rate and PGT-A or PGT-M/SR cycles. The occurrence of 3PN seems to impair the developing blastocyst and interfere with good embryo formation rate and mosaic rate in PGT. But the occurrence of 3PN does not seem to impair the euploid rate and aneuploid rate.

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Section: Discussionmentioning

confidence: 96%

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Jiang

Yuan

et al. 2023

Preprint

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“…First, delayed blastulation (time from fading of pronuclei to blastocyst stage) occurs in embryos with multi-pronuclei (PN) in bovine models [ 95 ] and is associated with abnormal fertilization [ 96 ]. Multi-PNs in human zygotes are also associated with high rates of aneuploidy [ 97 , 98 ]. Multi-PN zygotes were not excluded from this study, and thus, the presence of multi-PN could have explained why the aged embryos had delayed blastulation.…”

Section: Discussionmentioning

confidence: 99%

Machine learning in time-lapse imaging to differentiate embryos from young vs old mice

Yang,

Leynes,

Pawelka

et al. 2024

Biology of Reproduction

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Time-lapse microscopy for embryos is a non-invasive technology used to characterize early embryo development. This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the XGBoost (extreme gradient boosting) algorithm successfully predicted the age-related phenotype with 0.78 accuracy, 0.81 precision, and 0.83 recall following hyperparameter tuning. These results highlight two main scientific insights: maternal aging affects embryonic development pace, and that AI can differentiate between embryos from aged and young maternal mice by a non-invasive approach. Thus, machine learning can be used to identify morphokinetics phenotypes for further studies. This study has potential for future applications in selecting human embryos for embryo transfer, without or in complement with preimplantation genetic testing.

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Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Takahashi

Hirata

Otsuki

et al. 2022

Reprod Medicine & Biology

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Purpose This study aimed to analyze whether tripronuclear (3PN) zygotes, with two normal‐sized PNs and an additional smaller PN (2.1PN), can be used for embryo transfer. Method(s) A retrospective embryo cohort study was conducted on 695 patients who underwent intracytoplasmic sperm injection treatment. Blastocyst formation rates were compared between 2.1PN and 2PN zygotes and PGT‐A analysis was performed on 15 blastocysts derived from 2.1PN zygotes. Result(s) Blastocyst formation rates were comparable between 2.1PN (43.8%) and 2PN zygotes (54.8%; p = 0.212). The rates of blastocysts with good morphology derived from 2.1 PN and 2PN zygotes were 18.8% and 25.5%, respectively. No significant differences were detected ( p = 0.383). All of the analyzed blastocysts were diploid; however, 13 of these were found to be aneuploid, with a further two being mosaic. Conclusion Our results suggest that 2.1PN embryos can reach blastocyst stage. These blastocysts were diploid, however, predominantly aneuploid, and therefore could not be used for embryo transfer.

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Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Cited by 7 publications

References 22 publications

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Machine learning in time-lapse imaging to differentiate embryos from young vs old mice

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

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