Area postrema undergoes dynamic postnatal changes in mice and humans

Gokozan, Hamza; Baig, Faisal; Corcoran, Sarah; Catacutan, Fay Patsy; Gygli, Patrick; Takakura, Ana C.; Moreira, Thiago S.; Czeisler, Catherine; Otero, José

doi:10.1002/cne.23903

Cited by 11 publications

(14 citation statements)

References 49 publications

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“…While the CCK effect is unrelated to the neurotrophic actions observed in our current study, these behavioral findings indicate a prenatal development of the neuronal circuitry of the dorsal vagal complex. This prenatal development is further supported by neuroanatomical studies ( 27 ) and contrasts with the postnatal maturation of hypothalamic circuits ( 28 ).…”

Section: Discussionmentioning

confidence: 86%

Involvement of Amylin and Leptin in the Development of Projections from the Area Postrema to the Nucleus of the Solitary Tract

et al. 2017

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The area postrema (AP) and the nucleus of the solitary tract (NTS) are important hindbrain centers involved in the control of energy homeostasis. The AP mediates the anorectic action and the inhibitory effect on gastric emptying induced by the pancreatic hormone amylin. Amylin’s target cells in the AP project to the NTS, an integrative relay center for enteroceptive signals. Perinatal hormonal and metabolic factors influence brain development. A postnatal surge of the adipocyte-derived hormone leptin represents a developmental signal for the maturation of projections between hypothalamic nuclei controlling energy balance. Amylin appears to promote neurogenesis in the AP in adult rats. Here, we examined whether amylin and leptin are required for the development of projections from the AP to the NTS in postnatal and adult mice by conducting neuronal tracing studies with DiI in amylin- (IAPP−/−) and leptin-deficient (ob/ob) mice. Compared to wild-type littermates, postnatal (P10) and adult (P60) IAPP−/− mice showed a significantly reduced density of AP-NTS projections. While AP projections were also reduced in postnatal (P14) ob/ob mice, AP-NTS fiber density did not differ between adult ob/ob and wild-type animals. Our findings suggest a crucial function of amylin for the maturation of neuronal brainstem pathways controlling energy balance and gastrointestinal function. The impaired postnatal development of neuronal AP-NTS projections in ob/ob mice appears to be compensated in this experimental model during later brain maturation. It remains to be elucidated whether an amylin- and leptin-dependent modulation in neuronal development translates into altered AP/NTS-mediated functions.

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Section: Discussionmentioning

confidence: 86%

Involvement of Amylin and Leptin in the Development of Projections from the Area Postrema to the Nucleus of the Solitary Tract

et al. 2017

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“…Other studies have employed unbiased stereology on immunofluorescent confocal images (Mokin and Keifer, ). Our laboratory previously utilized unbiased stereology to quantify HA+ innervations to the area postrema (Gokozan et al, ). We have previously applied this unbiased stereology expertise to the quantification of structures stained with immunofluorescent labeling but found the methodology difficult to employ and less intuitive than the workflow presented in the current study.…”

Section: Discussionmentioning

confidence: 99%

Development of a Novel FIJI‐Based Method to Investigate Neuronal Circuitry in Neonatal Mice

Liu

Fair

Kaya

et al. 2018

Developmental Neurobiology

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The emergence of systems neuroscience tools requires parallel generation of objective analytical workflows for experimental neuropathology. We developed an objective analytical workflow that we used to determine how specific autonomic neural lineages change during postnatal development. While a wealth of knowledge exists regarding postnatal alterations in respiratory neural function, how these neural circuits change and develop in the weeks following birth remains less clear. In this study, we developed our workflow by combining genetic mouse modeling and quantitative immunofluorescent confocal microscopy and used this to examine the postnatal development of neural circuits derived from the transcription factors NKX2.2 and OLIG3 into three medullary respiratory nuclei. Our automated FIJI-based image analysis workflow rapidly and objectively quantified synaptic puncta in user-defined anatomic regions. Using our objective workflow, we found that the density and estimated total number of Nkx2.2-derived afferents into the pre-Bötzinger Complex significantly decreased with postnatal age during the first three weeks of postnatal life. These data indicate that Nkx2.2-derived structures differentially influence pre-Bötzinger Complex respiratory oscillations at different stages of postnatal development.

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“…Confocal microscopy instrumentation included a Zeiss LSM 700 microscope with a high‐resolution monochrome camera and Zeiss Zen software as described previously (Gokozan et al, ). Images were captured as.czi images and opened in ImageJ (RRID: SciRes_000137) for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Surface topography during neural stem cell differentiation regulates cell migration and cell morphology

Czeisler

Short

Nelson

et al. 2016

J of Comparative Neurology

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We sought to determine the contribution of scaffold topography to the migration and morphology of neural stem cells by mimicking anatomical features of scaffolds found in vivo. We mimicked two types of central nervous system scaffolds encountered by neural stem cells during development in vitro by constructing different diameter electrospun polycaprolactone (PCL) fiber mats, a substrate that we have shown to be topographically similar to brain scaffolds. We compared the effects of large fibers (made to mimic blood vessel topography) with those of small-diameter fibers (made to mimic radial glial process topography) on the migration and differentiation of neural stem cells. Neural stem cells showed differential migratory and morphological reactions with laminin in different topographical contexts. We demonstrate, for the first time, that neural stem cell biological responses to laminin are dependent on topographical context. Large-fiber topography without laminin prevented cell migration, which was partially reversed by treatment with rock inhibitor. Cell morphology complexity assayed by fractal dimension was inhibited in nocodazole- and cytochalasin-D-treated neural precursor cells in large-fiber topography, but was not changed in small-fiber topography with these inhibitors. These data indicate that cell morphology has different requirements on cytoskeletal proteins dependent on the topographical environment encountered by the cell. We propose that the physical structure of distinct scaffolds induces unique signaling cascades that regulate migration and morphology in embryonic neural precursor cells. J. Comp. Neurol. 524:3485-3502, 2016. © 2016 Wiley Periodicals, Inc.

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Area postrema undergoes dynamic postnatal changes in mice and humans

Cited by 11 publications

References 49 publications

Involvement of Amylin and Leptin in the Development of Projections from the Area Postrema to the Nucleus of the Solitary Tract

Involvement of Amylin and Leptin in the Development of Projections from the Area Postrema to the Nucleus of the Solitary Tract

Development of a Novel FIJI‐Based Method to Investigate Neuronal Circuitry in Neonatal Mice

Surface topography during neural stem cell differentiation regulates cell migration and cell morphology

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