Arecoline, Hesperidin and Trifluoperazine-mediated Cytotoxicity and Cell Death Potential in NIH/3T3 Fibroblasts Cells –Toxicity/Safety Assessment in a NIH/3T3 Model Fibroblast Cell Line

Varadharaj, Vembuli A; Pk, Suresh

doi:10.5530/ijper.54.3s.153

Cited by 1 publication

(1 citation statement)

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“…In a previous study, arecoline was found to stimulate A549 and CL1‐0 lung cancer cell migration (Chang et al, 2019). Another study of arecoline cytotoxicity using the MTT assay reported a reproducible IC 50 value of 38 μM in NIH/3T3 cells (Varadharaj & Pk, 2020). Furthermore, Gu et al, (2019) investigated the effect of arecoline on the cell proliferation of HaCaT cells.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the inhalation toxicity of arecoline benzoate aerosol in rats

Chu

Deng

et al. 2022

J of Applied Toxicology

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Arecoline is a pharmacologically active alkaloid isolated from Areca catechu. There are no published data available regarding the inhalation toxicity of arecoline in animals.This study aimed to evaluate the inhalation toxicity of arecoline in vitro and in vivo.For this purpose, arecoline benzoate (ABA) salt was prepared to stabilize arecoline in an aerosol. The MTT assay determined the half-maximal inhibitory concentration values of ABA and arecoline in A549 cell proliferation to be 832 and 412 μg/ml, respectively. The toxicity of acute and subacute inhalation in Sprague-Dawley rats was evaluated using the guidelines of the Organization for Economic Cooperation and Development. For acute inhalation, the median lethal concentration value of ABA solvent was >5175 mg/m 3 . After the exposure and during the recovery period, no treatment-related clinical signs were observed. In the 28-Day inhalation toxicity test, daily nose-only exposure to 2510 mg/m 3 aerosol of the ABA solvent contained 75 mg/m 3 ABA for male rats and 375 mg/m 3 ABA for female rats, which caused no observed adverse effects, except for the decreased body weight gain in male rats exposed to 375 mg/m 3 ABA. In this study, the no observed adverse effect level (NOAEL) for the 28-day repeated dose inhalation of ABA aerosol was calculated to be around 13 mg/kg/day for male rats and 68.8 mg/kg/day for female rats, respectively.

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Section: Discussionmentioning

confidence: 99%