'Argatroban' Monitoring in Human Plasma: aPTT and PiCT Studies on QCM-D vs 'Gold Standard'

Hussain, Munawar

doi:10.20510/ukjpb/3/i6/87834

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…The resonant frequency of the BAW transducer is sensitive to the small mass addition on the upper surface or the change in the viscosity of adjacent mediums. QCM devices have been successfully used to monitor the blood coagulation in real time [ 9 ] and measure several hemostatic parameters, including activated partial thromboplastin time (aPTT) [ 10 ], prothrombinase induced clotting time (PiCT) [ 11 ], thrombin times (TTs) [ 12 ], prothrombin time (PT) [ 13 ] and fibrinogen [ 14 ]. This technology shows an outstanding application prospect in the point-of-care (POC) system.…”

Section: Introductionmentioning

confidence: 99%

ZnO Film Bulk Acoustic Resonator for the Kinetics Study of Human Blood Coagulation

Chen

Zhang

et al. 2017

Sensors

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Miniaturized and rapid blood coagulation assay technologies are critical in many clinical settings. In this paper, we present a ZnO film bulk acoustic resonator for the kinetic analysis of human blood coagulation. The resonator operated in thickness shear resonance mode at 1.4 GHz. When the resonator contacted the liquid environment, the viscous loading effect was considered as the additional resistance and inductance in the equivalent circuits, resulting in a linear relationship with a slope of approximately −217 kHz/cP between the liquid viscosity and the frequency of the resonator. The downshift of the resonant frequency and the viscosity change during the blood coagulation were correlated to monitor the coagulation process. The sigmoidal trend was observed in the frequency response for the blood samples activated by thromboplastin and calcium ions. The coagulation kinetics involving sequential phases of steady reaction, growth and saturation were revealed through the time-dependent frequency profiles. The enzymatic cascade time, the coagulation rate, the coagulation time and the clot degree were provided by fitting the time-frequency curves. The prothrombin times were compared with the results measured by a standard coagulometer and show a good correlation. Thanks to the excellent potential of integration, miniaturization and the availability of direct digital signals, the film bulk acoustic resonator has promising application for both clinical and personal use coagulation testing technologies.

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Section: Introductionmentioning

confidence: 99%

ZnO Film Bulk Acoustic Resonator for the Kinetics Study of Human Blood Coagulation

Chen

Zhang

et al. 2017

Sensors

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“…It is attractive due to its cost-effectiveness on compared to its counterpart biosensor platforms 15 . [18][19][20][21] . Application of aPTT to whole blood for (any)…”

Section: Introductionmentioning

confidence: 99%

aPTT: 1st Recognition for Human Whole Blood on QCM-D Platform

Hussain

2015

PBJ

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Activated partial thromboplastin time (aPTT) assay for whole blood on quartz crystal microbalance with dissipation (QCM-D) platform has been recognized for the first time. QCM-D platform is studied in parallel with 'gold standard' mechanical coagulometer in the perspective of anticoagulant bio-sensing. In this report, the lowest sample volume application of 1.66 µL of human whole blood for aPTT has been demonstrated. Mechanical coagulomter uses 60 times higher whole blood (and each reagent) volume application of 100 µL for aPTT laboratory experiments. This study is important in the terms of its robustness due to direct whole blood method and its cost-effectiveness due to lowest sample (and reagents) volume application. This

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“…QCM is well known due to its cost-effectiveness for biosensor applications on comparing to its counterpart sensor techniques 14 . Recently, haemostasis [15][16][17][18][19][20] Technoclone gmbH (Austria).…”

Section: Introductionmentioning

confidence: 99%

A Simultaneous Monitoring of Coagulation Time and Fibrinogen via PiCT on QCM-D

Hussain

2015

PBJ

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'Argatroban' Monitoring in Human Plasma: aPTT and PiCT Studies on QCM-D vs 'Gold Standard'

Abstract: Low-molecular weight heparins (LMWHs) are used as effective and safe alternative anticoagulants to unfractionated heparin (UFH) in surgery. Argatroban is an important example of such kind of anticoagulant and is the most-used anticoagulant in surgery after UFH. This is first

Cited by 7 publications

References 23 publications

ZnO Film Bulk Acoustic Resonator for the Kinetics Study of Human Blood Coagulation

ZnO Film Bulk Acoustic Resonator for the Kinetics Study of Human Blood Coagulation

aPTT: 1st Recognition for Human Whole Blood on QCM-D Platform

A Simultaneous Monitoring of Coagulation Time and Fibrinogen via PiCT on QCM-D

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