1993
DOI: 10.1007/bf02543018
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Argentation supercritical fluid chromatography for quantitative analysis of triacylglycerols

Abstract: A method for quantitative analysis of neutral lipids has been developed. Four different techniques have been combined for this purpose—supercritical fluid chromatography (SFC), silver ion chromatography, packed microcolumns and miniaturized evaporative light‐scattering detection (ELSD). The development and optimization of the method are discussed. The separation of a series of vegetable, fish and hydrogenated oils was demonstrated. Application of eluent composition programming resulted in excellent separation … Show more

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Cited by 42 publications
(14 citation statements)
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“…Separations were not achieved of triglycerides having both the same partition number and total chain length (SSO/PGaO), or of triglycerides differing only in the position of the double bonds in one fatty acid moity (alinolenate: A9c, A 12c, A 15c or 7-1inolenate: A6c, A9c, A12c). This last separation was reached either by NARP-LC with a packed Brownlee spheri-50DS, that involves the use of an evaporative light scattering detector [26], or by using argentation SFC [27], that requires temperature or modifier percentage gradients. Additional studies should be carried out overcome these limits in SubFC with packed octadecyl bonded phases, for instance, by using different coupled stationary phases.…”
Section: Final Optimizationmentioning
confidence: 99%
“…Separations were not achieved of triglycerides having both the same partition number and total chain length (SSO/PGaO), or of triglycerides differing only in the position of the double bonds in one fatty acid moity (alinolenate: A9c, A 12c, A 15c or 7-1inolenate: A6c, A9c, A12c). This last separation was reached either by NARP-LC with a packed Brownlee spheri-50DS, that involves the use of an evaporative light scattering detector [26], or by using argentation SFC [27], that requires temperature or modifier percentage gradients. Additional studies should be carried out overcome these limits in SubFC with packed octadecyl bonded phases, for instance, by using different coupled stationary phases.…”
Section: Final Optimizationmentioning
confidence: 99%
“…The technique has not yet been used for the analysis of samples containing both ot-linolenic acid (18:3n-3) and T-linolenic acid (18:3n-6) moieties in their triacylglycerols, although resolution of ester derivatives of cxand T-linolenic acids has been reported (18). Few studies of oils rich in y-linolenic acid have been conducted by silver-ion chromatography, for example, evening primrose oil by silverion HPLC (19) and silver-ion TLC (20), and borage oil by silver-ion supercritical fluid chromatography (21). Triacylglycerols of the seeds of the plants belonging to the genus Ribes are relatively abundant both in T-and cz-linolenic acids.…”
mentioning
confidence: 99%
“…Later on, Christie et al [115] reported on a novel coating procedure of SIs on silica-based cation exchanger, resulting in higher stability of the columns. Such silver-impregnated cation exchanger phases have been early employed, for lipid separation under SFC conditions, by the group of Blomberg et al using either fused-silica capillary tubing as column material, or glass-lined metal tubing [ 88,89]. These packed microcolumns (0.7 or 0.25 mm id) provided a low flow of mobile phase and low thermal mass, which matched the geometry of miniaturized ELSD and allowed for rapid and efficient thermal gradients to be applied.…”
Section: Separation Of Tagsmentioning
confidence: 99%
“…Separation of TAG regioisomers remains an issue, which can be addressed either by the use of SI chromatography, or by careful plan and interpretation of tandem MS data, since fragmentation is more abundant at the sn ‐1 and sn ‐3 positions, compared to the sn ‐2. SI packed columns have been employed as the stationary phase in SFC with UV, ELSD , or MS detection . On SI exchange columns (SIC), TAG separation is controlled by the degree of unsaturation, and the distribution of the DBs in the FAs.…”
Section: Applications Of Sfc To Lipid Analysismentioning
confidence: 99%