Arginase Status in Ram Reproductive System

Razmi, Nematollah; Gh.A., Jeloudar; Nazifi, Saeed; A.A.R., Dehghan

doi:10.1080/09712119.2004.9706506

Cited by 9 publications

(15 citation statements)

References 5 publications

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“…Arginase (mitochondrial form) plays an important role in the regulation of nitric oxide synthases, polyamines, glutamate and proline (Jenkinson, Grody, & Cederbaum, ). The mitochondrial form of arginase has been reported to exist in different sections of the reproductive system (Razmi, Jelodar, Nazifi, & Dehghani, ). Polyamines are also found in male reproductive organs and tissues, as well as in seminal plasma (Oefner et al, ) and spermatozoa (Rubinstein & Breitbart, ), semen, prostate, epididymis and testis (Keskinege, Elgün, & Yilmaz, ).…”

Section: Introductionmentioning

confidence: 99%

Influence of vitamin E and vitamin E‐selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen

Kaya

Gür

Erişir

et al. 2019

Reprod Domestic Animals

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The effects of vitamin E and vitamin E‐selenium combination on seminal plasma arginase activity and nitric oxide level and some spermatological properties in rams were investigated in this study. For control group, animals were injected intramuscularly with physiological saline. For vitamin E group, rams were injected intramuscularly with 300 mg/ram vitamin E. For vitamin E + selenium group, animals were injected intramuscularly with 5 ml/ram vitamin E + selenium. The semen was collected by artificial vagina at 1st, 4th, 24th, 48th and 72nd hr after administration in each group. Significant decreases in seminal plasma arginase activity (at 1st, 24th and 48th hr), nitric oxide level (at 72nd hr) and abnormal sperm rate (at 1st, 24th and 72nd hr), and significant increases in semen volume (at 24th hr), semen mass activity (at 24th and 48th hr), sperm motility (at 24th, 48th and 72nd hr) and concentration (at 1st hr) were observed in vitamin E group compared with control group. Similarly, significant increase in semen volume (at 1st, 24th and 48th hr), mass activity, (at 48th hr), motility (at 48th and 72nd hr) and concentration (at 4th, 24th and 48th hr), and significant decrements in abnormal sperm rate (at 1st, 24th, 48th and 72nd hr), seminal plasma nitric oxide level (at 1st, 4th, 24th and 48th hr) and semen pH (at 24th and 48th hr) were detected in vitamin E + selenium group in comparison to the control group. As a result, it is suggested that vitamin E and/or vitamin E + selenium applications may improve reproductive performance.

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Section: Introductionmentioning

confidence: 99%

Influence of vitamin E and vitamin E‐selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen

Kaya

Gür

Erişir

et al. 2019

Reprod Domestic Animals

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“…Our data shows that arginase is present in all parts of the normal dog reproductive system with no significant differences. However, significant variation has been reported for ASA in different parts of the reproductive system of the ram [17]. In the diabetic dogs in this study, ASA increased significantly in the corpus cavernosum, corpus spongiosum, prostate, and epididymis.…”

Section: Discussionmentioning

confidence: 64%

“…Polyamines in turn may mediate the action of androgens [5]. Arginase activity has been reported in different parts of the reproductive systems of cattle [16], rams [17], and ewes [18]. The existence of multiple forms of arginase in eukaryotes suggests a complex regulatory role for this enzyme in the metabolism, development, and maintenance of these organisms.…”

mentioning

confidence: 99%

Arginase Alteration in the Reproductive System of Alloxan-Diabetic Dogs

Jelodar

Razmi

Gholampour

2007

Journal of Reproduction and Development

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Abstract. This study was conducted to evaluate possible alteration in the activity of arginase, an important enzyme of cell proliferation and vascular smooth muscle contraction regulator in diabetics, that may be correlated with low fertility in diabetic patients. In this investigation, 6 apparently healthy adult male dogs were selected and divided in two groups, diabetics and non-diabetics. Diabetes mellitus was induced in one group by intravenous (IV) injection of alloxan (100 mg/kg). Dogs with a fasting blood glucose (FBS) of more than 200 mg/dl were considered to be diabetic. Four weeks following induction of diabetes mellitus, the animals in both groups were anesthetized by an IV injection of sodium thiopental. Livers and whole reproductive systems, including the testes, penis, urethra, and prostate, were dissected. The epididymides, corpus cavernosum, corpus spongiosum, penile urethra, and vas deferens were also dissected and removed from the reproductive system. Arginase activity and total protein were measured by the urea and Lowry's methods respectively in above mentioned sections. Plasma testosterone was determined by the radioimmunoassay method. The results showed significantly (P<0.05) increased arginase specific activity (ASA) in the liver, epididymis, prostate, corpus cavernosum and corpus spongiosum of the diabetic dogs. In the reproductive system of the diabetic dog, the maximum and minimum ASA was seen in the corpus cavernosum and testes, respectively (105.12 ± 8.76 vs. 25.0 ± 0.55). No such variation was observed in the ASA of normal dogs (39.0 ± 5.47 vs. 25.0 ± 5.47). There was no significant difference in plasma testosterone level between the groups. In conclusion, diabetes increased the ASA in liver, prostate, epididymis, corpora cavernosa, and corpora spongiosum of the male dogs and may contribute to erectile dysfunction or low fertility in diabetics. Key words: Arginase, Diabetes mellitus, Dog, Reproductive system (J. Reprod. Dev. 53: [317][318][319][320][321] 2007) iabetes mellitus (DM) is a chronic endocrine/ m e t a b o l i c d i s o r d e r w i t h d e v e l o p i n g pathological complications. The major chronic complications associated with diabetes include retinopathy, neuropathy, nephropathy, and atherosclerosis in the coronary and peripheral circulation [1]. Lower pregnancy rates have been associated with basic fertility potential in diabetic men, and variations in semen quality have been reported in these patients [2,3]. Semen analysis may reveal decreased sperm motility and density, abnormal morphology, and generally increased seminal plasma abnormalities. In addition, diabetic men may have decreased serum testosterone levels due to impaired Leydig cell function [4] or a decrease in polyamines, which are required for androgen synthesis [5].Insulin can cross the blood-testis barrier, but its level in seminal plasma has not been shown to correlate significantly with semen parameters [6]. Abnormal sperm morphology in diabetic men is

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“…It has been determined that arginase enzyme activity has different levels in all parts of the ram reproductive system. Arginase activity has been reported to >60 IU/mg protein in the mucosal layers of the ureter and at least 9–10 to 20–31 IU/mg protein in the seminal fluid and bulbourethral gland (Razmi, Jelodar, Nazifi, & Dehghani, ) Arginase enzyme was found in the prostate, testes and seminal plasma in mammals, in other parts of the reproductive system and in the epididymal spermatozoa in the bulls (Razmi, Jelodar, Nazifi, & Dehghani, ) and in the rams (Mendez & Martinez, ).…”

Section: Discussionmentioning

confidence: 99%

Effect ofl-arginine addition on long-term storability of ram semen

2017

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This study was conducted to investigate the effect of l-arginine addition on long-term storability of ram semen. Six Akkaraman rams were used as material. Semen samples were collected. Pooled samples were diluted and were divided into six equal aliquots. While aliquot 1 was kept as control, the stock solutions including 0.1, 0.5, 1, 5 and 10 mm l-arginine were added to other aliquots. All aliquots were routinely frozen in 0.25-ml straws at -130°C liquid nitrogen vapour and stored in liquid nitrogen -196°C until being analysed. The equilibrated and thawed sperm motility, membrane integrity and arginase activity were evaluated. While the 10 mm l-arginine supplementation significantly (p < .001) decreased equilibrated sperm motility, the 5 mm significantly (p < .05) increased the membrane integrity and arginase activity in comparison with the control group. The motility (p < .001) and membrane integrity (p < .01) were determined to be highest in 0.5 mm group, while significant reductions were observed in motility (p < .001) of 10 mm group and arginase activity (p < .05) of 1, 10 mm groups as compared to the control group. It was concluded that in vitro addition of 0.5 mm l-arginine to ram semen may be useful, but 10 mm may be harmful to spermatozoa quality during long-term storage.

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Arginase Status in Ram Reproductive System

Cited by 9 publications

References 5 publications

Influence of vitamin E and vitamin E‐selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen

Influence of vitamin E and vitamin E‐selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen

Arginase Alteration in the Reproductive System of Alloxan-Diabetic Dogs

Effect ofl-arginine addition on long-term storability of ram semen

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