Arginine Supplementation Induces Arginase Activity and Inhibits TNF‐α Synthesis in Mice Spleen Macrophages After Intestinal Obstruction

Quirino, Iara Eliza Pacífico; Carneiro, Mariângela; Cardoso, Valbert Nascimento; Santos, Rosana das Graças Carvalho dos; Vieira, Leda Quércia; Fiuza, Jacqueline Araújo; Alvarez-Leite, Jacqueline I.; Generoso, Simone de Vasconcelos; Correia, María Isabel Toulson Davisson

doi:10.1177/0148607114546374

Cited by 13 publications

(7 citation statements)

References 25 publications

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“…Therefore, the ROS-scavenging property of Arg together with its ability to sustain spare endogenous antioxidants and decrease activity of the hepatic lipogenic enzyme may inhibit oxidative liver damage and decrease inflammation status [ 6 , 47 , 52 ]. Recent studies were reported that Arg inhibits uncontrolled synthesis of TNF-α thus block its deleterious effects [ 53 , 54 ]. The experimental study of Ozsoy et al (2011) [ 55 ] demonstrated that i.p.…”

Section: Discussionmentioning

confidence: 99%

Investigation into the antioxidant role of arginine in the treatment and the protection for intralipid-induced non-alcoholic steatohepatitis

et al. 2015

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BackgroundThis study investigated the possible roles of arginine (Arg) in ameliorating oxidative damage of intralipid (IL)-induced steatohepatitis (NASH).MethodsNASH was induced in Sprague-Dawley rats by intravenous administration of 20 % IL for three weeks and then rats were pre- and post-treated with intraperitoneal injection of Arg for two weeks. Several biochemical parameters (blood and hepatic lipid peroxidation, glutathione, glutathione peroxidase and superoxide dismutase, hepatic cytochrome P450 2El monooxygenase (CYP2E1), nitric oxide (NO), endothelial nitric oxide synthase (eNOS) and tumor necrosis factor-α “TNF-α”) and liver histopathology were detected for rat groups.ResultsThe administration of Arg either before or after IL significantly ameliorated uncontrolled elevation of TBARS content, CYP2E1 activity (0.32 ± 0.01 or 0.3 ± 0.02 IU/mg) and TNF-α level. These effects were associated with a significant increase in the levels of glutathione, activities of antioxidant enzymes, NO level (1.649 ± 0.047 or 1.957 ± 0.073 μmol/g) and activity of hepatic eNOS (0.05 ± 0.002 or 0.056 ± 0.002 IU/mg) compared to the IL-treated rats. Moreover, the injection of Arg in NASH-induced rats showed normal hepatocytes, no steatosis and no bile duct proliferation but mild inflammation in the group which received IL after Arg.ConclusionsThese results proved that pre- and post-treatment with Arg blocked oxidative stress-induced NASH by inhibiting CYP2E1 activity, decreasing TNF- α level and restoration activities of eNOS and antioxidant enzymes as well as glutathione level. This antioxidant effect of Arg leads to reverse signs of liver pathology of NASH with amelioration of liver and kidney functions.

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Section: Discussionmentioning

confidence: 99%

Investigation into the antioxidant role of arginine in the treatment and the protection for intralipid-induced non-alcoholic steatohepatitis

et al. 2015

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“…It was also able to inhibit the production of pro-inflammatory mediator (tumor necrosis factor-α (TNF-α)) in mice spleen macrophages after intestinal obstruction (Quirino et al, 2014), as well as to improve the production of NO in diabetic-obese rats (Breuillard et al, 2012). Nevertheless, it was discovered that L-arginine promotes cardiac and muscle fibrosis in muscular dystrophy via the regulation of macrophage metabolism (Wehling-Henricks et al, 2010), and it serves as a potential pro-inflammatory agent in mice peritoneal macrophages (Pekarova et al, 2013).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The crucial role of l-arginine in macrophage activation: What you need to know about it

Pekarová

Lojek

2015

Life Sciences

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“…It has been proven that regular exercise training decreases inflammatory cytokines 38 . Likewise, Quirino et al (2014) showed L-arginine inhibits uncontrolled synthesis of TNF-α thus block its deleterious effects 39 . It is likely that anti-inflammatory cytokine (IL-10)-mediated upregulation of anti-oxidant defenses (such as Cu/ZnSOD) via Nrf2 may represent the underlying mechanism for exercise-induced reduction of oxidative stress 40 .…”

Section: Discussionmentioning

confidence: 99%

Aerobic training and L-arginine supplement attenuates myocardial infarction-induced kidney and liver injury in rats via reduced oxidative stress

Ranjbar

Nazem

Sabrinezhad

et al. 2018

Indian Heart Journal

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Arginine Supplementation Induces Arginase Activity and Inhibits TNF‐α Synthesis in Mice Spleen Macrophages After Intestinal Obstruction

Abstract: Arginine supplementation and iNOS inhibition led to increased arginase activity. Arginine availability decreased plasma TNF-α levels, which may be directly related to nitric oxide derived from arginine.

Cited by 13 publications

References 25 publications

Investigation into the antioxidant role of arginine in the treatment and the protection for intralipid-induced non-alcoholic steatohepatitis

Investigation into the antioxidant role of arginine in the treatment and the protection for intralipid-induced non-alcoholic steatohepatitis

The crucial role of l-arginine in macrophage activation: What you need to know about it

Aerobic training and L-arginine supplement attenuates myocardial infarction-induced kidney and liver injury in rats via reduced oxidative stress

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