Argon‐based atmospheric pressure plasma enhances early bone response to rough titanium surfaces

Coelho, Paulo G.; Giro, Gabriela; Teixeira, Hellen S.; Marin, Charles; Witek, Lukasz; Thompson, Van P.; Tovar, Nick; Silva, Nelson R.F.A.

doi:10.1002/jbm.a.34127

Cited by 99 publications

(95 citation statements)

References 49 publications

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“…Plasma processes are often applied for cleaning and sterilization of dental implants after other surface treatments have been carried out, but they have also been tested for the acceleration of osseointegration [28][29][30][31] and the application of antibacterial properties to implants [28,29,32]. Argon and oxygen are the most used gases for these applications [28][29][30][31][32][33]. Plasma treatments also include plasma immersion ion implantation (PIII) processes, where plasma is used to produce ions, which are accelerated towards the surface of the treated sample and are implanted into it [33].…”

Section: Surface Treatmentsmentioning

confidence: 99%

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

et al. 2016

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Surface modification of dental implants is a key process in the production of these medical devices, and especially titanium implants used in the dental practice are commonly subjected to surface modification processes before their clinical use. A wide range of treatments, such as sand blasting, acid etching, plasma etching, plasma spray deposition, sputtering deposition and cathodic arc deposition, have been studied over the years in order to improve the performance of dental implants. Improving or accelerating the osseointegration process is usually the main goal of these surface processes, but the improvement of biocompatibility and the prevention of bacterial adhesion are also of considerable importance. In this review, we report on the research of the recent years in the field of surface treatments and coatings deposition for the improvement of dental implants performance, with a main focus on the osseointegration acceleration, the reduction of bacterial adhesion and the improvement of biocompatibility.

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Section: Surface Treatmentsmentioning

confidence: 99%

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

et al. 2016

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“…10 When the Ti surface experienced various types of APP treatments, the APP eliminated surface carbon contaminations (i.e., activation), increased surface wettability, facilitated adsorption of FN and, consequently, facilitated cell adhesion and proliferation, including those of osteoblastic cells. 5,8,[11][12][13][14] However, to our knowledge, detailed mechanistic investigations from a biological perspective have not been performed. To provide additional data and to better understand the effects of APP, we used a nitrogen gas-based APP system in this study to modify a chemically polished commercially pure Ti (CP-cpTi) surface and examined the responses of human prenatal-derived osteoblastic cells to the APP-treated CP-cpTi surface at the molecular level.…”

Section: Introductionmentioning

confidence: 95%

“…Correspondence to: T. Kawase (e-mail: kawase@dent.niigata-u.ac.jp) Contract grant sponsor: Grant-in-Aid for New Market Development by the Industrial Creation for 2012 from the Niigata Industrial Creation Organization and JSPS KAKENHI; contract grant number: 24390443 surface energy and to modify the oxide layer that interacts with the proteins and cells of the surrounding tissue. 8,9 Thus, plasma treatment is expected to improve the adhesion of the surrounding tissue. 4 However, the conventional plasma treatment method requires appropriate material sizes for the vacuum reactor and therefore limits the size and composition of the substrates to be modified.…”

Section: Introductionmentioning

confidence: 99%

An atmospheric‐pressure plasma‐treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal‐derived osteoblastic cells

et al. 2014

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An atmospheric-pressure plasma (APP) treatment was recently reported to render titanium (Ti) surfaces more suitable for osteoblastic cell proliferation and osteogenesis. However, the mechanism of action remains to be clearly demonstrated. In this study, we focused on cell adhesion and examined the effects of the APP treatment on the initial responses of human prenatal-derived osteoblastic cells incubated on chemically polished commercially pure Ti (CP-cpTi) plates. In the medium containing 1% fetal bovine serum, the initial cell adhesion and the actin polymerization were evaluated by scanning electron microscopy and fluorescence microscopy. The expression of cell adhesion-related molecules and osteoblast markers at the messenger RNA level was assessed by real-time quantitative polymerase chain reaction. Although the cells on the APP-treated CP-cpTi surface developed fewer cytoskeletal actin fibers, they attached with higher affinity and consequently proliferated more actively (1.46-fold over control at 72 h). However, most of the cell adhesion molecule genes were significantly downregulated (from 40 to 85% of control) in the cells incubated on the APP-treated CP-cpTi surface at 24 h. Similarly, the osteoblast marker genes were significantly downregulated (from 49 to 63% of control) at 72 h. However, the osteoblast marker genes were drastically upregulated (from 197 to 296% of control) in these cells by dexamethasone and β-glycerophosphate treatment. These findings suggest that the APP treatment improves the ability of the CP-cpTi surface to support osteoblastic proliferation by enhancing the initial cell adhesion and supports osteoblastic differentiation when immature osteoblasts begin the differentiation process.

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“…Argon is chemically very inactive and has been used to provide an inert atmosphere during deposition. It has been shown that argon protection could effectively reduce the air contaminants on acid-etched Ti implant surfaces and maintain the surface hydrophilicity and biological activity of implants [30,31], enhancing the early bone formation on Ti surfaces [30,32]. Nitrogen is a chemically neutral gas and does not change the biological properties of the samples.…”

Section: Introductionmentioning

confidence: 99%

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

Kaitainen

Kröger

et al. 2016

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The chemical composition and texture of titanium coatings can influence the growth characteristics of the adhered cells. An enhanced proliferation of the human mesenchymal stem cells (hMSCs) would be beneficial. The present study was aimed to investigate whether titanium deposited at different atmospheres would affect the cell growth properties, cellular morphology, and expression of surface markers of hMSCs. Titanium-based coatings were deposited on silicon wafers under oxygen, nitrogen, or argon atmospheres by ultra-short pulsed laser deposition using two different gas pressures followed by heating at 400 °C for 2 h. The characteristics of the coated surfaces were determined via contact angle, zeta potential, and scanning electron microscopy (SEM) techniques. Human MSCs were cultivated on differently coated silicon wafers for 48 h. Subsequently, the cell proliferation rates were analyzed with an MTT assay. The phenotype of hMSCs was checked via immunocytochemical stainings of MSC-associated markers CD73, CD90, and CD105, and the adhesion, spreading, and morphology of hMSCs on coated materials via SEM. The cell proliferation rates of the hMSCs were similar on all coated silicon wafers. The hMSCs retained the MSC phenotype by expressing MSC-associated markers and fibroblast-like morphology with cellular projections. Furthermore, no significant differences could be found in the size of the cells when cultured on all various coated surfaces. In conclusion, despite certain differences in the contact angles and the zeta potentials of various titanium-based coatings, no single coating markedly improved the growth characteristics of hMSCs.

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Argon‐based atmospheric pressure plasma enhances early bone response to rough titanium surfaces

Cited by 99 publications

References 49 publications

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

An atmospheric‐pressure plasma‐treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal‐derived osteoblastic cells

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

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