ArgR of Streptomyces coelicolor Is a Versatile Regulator

Pérez‐Redondo, Rosario; Rodrı́guez-Garcı́a, Antonio; Botas, Alma; Santamarta, Irene; Martı́n, Juan F.; Liras, Paloma

doi:10.1371/journal.pone.0032697

Cited by 35 publications

(53 citation statements)

References 64 publications

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“…Interestingly, induction of the SCO5655 has been reported to be stimulated by diamide (Kallifidas et al, 2010), however, not by arginine (Perez-Redondo et al, 2012). Further genetic analysis of SCO5655 and other potential aminotransferase homologs (SCO1223, SCO1284, and SCO6769) would be required to determine whether a PatA-dependent pathway is functional.…”

Section: Discussionmentioning

confidence: 99%

Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145

et al. 2017

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Streptomyces coelicolor M145 was shown to be able to grow in the presence of high concentrations of polyamines, such as putrescine, cadaverine, spermidine, or spermine, as a sole nitrogen source. However, hardly anything is known about polyamine utilization and its regulation in streptomycetes. In this study, we demonstrated that only one of the three proteins annotated as glutamine synthetase-like protein, GlnA3 (SCO6962), was involved in the catabolism of polyamines. Transcriptional analysis revealed that the expression of glnA3 was strongly induced by exogenous polyamines and repressed in the presence of ammonium. The ΔglnA3 mutant was shown to be unable to grow on defined Evans agar supplemented with putrescine, cadaverine, spermidine, and spermine as sole nitrogen source. HPLC analysis demonstrated that the ΔglnA3 mutant accumulated polyamines intracellularly, but was unable to degrade them. In a rich complex medium supplemented with a mixture of the four different polyamines, the ΔglnA3 mutant grew poorly showing abnormal mycelium morphology and decreased life span in comparison to the parental strain. These observations indicated that the accumulation of polyamines was toxic for the cell. An in silico analysis of the GlnA3 protein model suggested that it might act as a gamma-glutamylpolyamine synthetase catalyzing the first step of polyamine degradation. GlnA3-catalyzed glutamylation of putrescine was confirmed in an enzymatic in vitro assay and the GlnA3 reaction product, gamma-glutamylputrescine, was detected by HPLC/ESI-MS. In this work, the first step of polyamine utilization in S. coelicolor has been elucidated and the putative polyamine utilization pathway has been deduced based on the sequence similarity and transcriptional analysis of homologous genes expressed in the presence of polyamines.

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Section: Discussionmentioning

confidence: 99%

Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145

et al. 2017

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“…Daniel Charlier (Vrije Universiteit Brussel) talked about the impact of the topological changes induced by PepA, leucine aminopeptidase A, at the carP1 promoter of the carAB operon in Escherichia coli which is a region known to be regulated by purines, pyrimidines and arginine (Minh et al, 2009). Paloma Liras Padin (Universidad de Leon) presented phenotypic characterization of ArgR deleted Streptomyces coelicolor and microarray analysis data revealing around 1500 genes that are derepressed due to lack of ArgR, including genes that are involved in nitrogen regulation (Perez-Redondo et al, 2012).…”

Section: Arginine and The Urea Cycle In Health And Diseasementioning

confidence: 99%

Cellular Serpents and Dreaming Spires: New Frontiers in Arginine and Pyrimidine Biology

Aughey¹,

Tastan²,

Liu³

2014

Journal of Genetics and Genomics

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“…Arg boxes in Streptomyces clavuligerus are imperfect palindromes comprised of two 18-nucleotide Arg boxes separated by 2 bp (25). Derepression of arginine and pyrimidine biosynthesis genes by argR deletion was shown using a multi-omics approach; however, the change in gene expression was indifferent to arginine supplementation (22). NdgR is a recently identified TF.…”

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confidence: 99%

NdgR, a Common Transcriptional Activator for Methionine and Leucine Biosynthesis in Streptomyces coelicolor

et al. 2012

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We show here that NdgR, a known transcriptional activator of isopropylmalate dehydratase in actinomycetes, may have other targets in the cell. An in-frame deletion mutant of ndgR showed unexpectedly poor growth in defined minimal medium even in the presence of leucine. To our surprise, it was supplementation of cysteine and methionine that corrected the growth. Based on this, we propose that NdgR induces cysteine-methionine biosynthesis. Direct involvement of NdgR in the very last steps of methionine synthesis with methionine synthase (metH) and 5,10-methylenetetrahydrofolate reductase (metF) was examined. From a pulldown assay, it was seen that NdgR was enriched from crude cell lysates with a strong affinity to metH and metF upstream sequences. Direct physical interaction of NdgR with these targets was further examined with a gel mobility shift assay. ndgR, leuC, metH, and metF were inducible in M145 cells upon nutrient downshift from rich to minimal medium but were not induced in the ndgR knockout mutant. Taking these observations together, NdgR-dependent metH-metF expression would account for the abnormal growth phenotype of the ndgR mutant although there may be additional NdgR-dependent genes in the Cys-Met metabolic pathways. As the first transcriptional factor reported for regulating Cys-Met metabolism in Streptomyces, NdgR links two disparate amino acid families, branched-chain amino acids (BCAAs) and sulfur amino acids, at the transcriptional level. Considering that Cys-Met metabolism is connected to mycothiol and one-carbon metabolism, NdgR may have broad physiological impacts.

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ArgR of Streptomyces coelicolor Is a Versatile Regulator

Cited by 35 publications

References 64 publications

Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145

Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145

Cellular Serpents and Dreaming Spires: New Frontiers in Arginine and Pyrimidine Biology

NdgR, a Common Transcriptional Activator for Methionine and Leucine Biosynthesis in Streptomyces coelicolor

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