Aristolochia bracteolata Enhances Wound Healing in vitro through Anti-inflammatory and Proliferative Effect on Human Dermal Fibroblasts and Keratinocytes

Girija, Dinesh Murugan; Kalachaveedu, Mangathayaru; Rajasekaran, Subbiah; Jenifer, Preethi; Rao, Suresh Ranga

doi:10.5530/pj.2017.6s.169

Cited by 14 publications

(9 citation statements)

References 21 publications

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“…This suggests that the extract accelerates wound healing by inducing the migration of fibroblasts. A similar study on another species of the same family, A. bracteolate, reported that its methanolic extract stimulated the migration of fibroblasts and kerationcyctes and enhanced the expression of wound healing related genes [23].…”

Section: Discussionmentioning

confidence: 77%

In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression

Bolla

Al‐Subaie

Al-Jindan

et al. 2019

Heliyon

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Background Identification and assessment of therapeutic potential of natural products derived from medicinal plants have led to the discovery of innovative and economical drugs to treat several diseases, including chronic wounds. In vitro cell based scratch assay is an appropriate and inexpensive method for initial understanding of wound healing potential of medicinal plant extracts. The current study was aimed at investigating the wound healing capacity of Aristolochia saccata leaf extract by using scratch assay as a primary model, where proliferative and migratory capabilities of test compounds could be monitored through microscopy studies. A. saccata is an evergreen climbing shrub belongs to the family Aristolochiaceae. Methods Methanolic extraction of the plant material was done using Soxhlet apparatus and the cytotoxicity of the extract on L929 cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. L929 is a human fibroblast cell line. In vitro scratch assay was performed to evaluate the wound healing properties of A. saccata leaf extract and possible mechanism of action was analyzed by flow cytometric expression studies of an extracellular matrix (ECM) factor, collagen type-1. Results MTT assay revealed that A. saccata leaf extract had no cytotoxic effect on the cells and at higher concentrations, the extract showed mild toxicity resulting in the death of just 2.88% cells. Scratch assay showed 34.05%, 70.00%, 93.52% wound closure at 12hrs, 24hrs and 48hrs of incubation respectively. These results were similar compared to positive control which showed 37.60, 56.41 and 99.05% of wound closure. Further, flow cytometry-based studies revealed that the A. saccata leaf extract induced the expression of ECM remodelling factor collagen-1. Conclusion Our study revealed the wound healing capabilities of A. saccata In vitro . Hence, A. saccata could be recommended as a potential source of wound healing agents.

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Section: Discussionmentioning

confidence: 77%

In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression

Bolla

Al‐Subaie

Al-Jindan

et al. 2019

Heliyon

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show abstract

“…Thus, proper proliferation and migration of fibroblasts are necessary factors to the formation of granulation tissue as the basis of new dermis. Previous studies have demonstrated that plant-based natural compounds that can stimulate migration of fibroblasts promote wound healing by helping to develop an optimized therapeutic protocol [56]. In this context, in this study, the effects of TS extracts on fibroblast migration were assessed by using scratch assay, which is a useful method to mimic the migration of cells during wound healing in vivo.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

et al. 2019

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Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H2O2) by increasing cell viability compared to only H2O2-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.

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“…4,[20][21][22][23] A previous study showed that extracts of leaves, stem bark and roots of K. africana and S. hispidus contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides which exhibit antimicrobial, antioxidant and enhanced wound healing properties. 24 Likewise, Agyare et al reported the presence of tannins, flavonoids, alkaloids, and glycosides in J. flava and L. welwitschii extracts and their antimicrobial and wound healing properties. 25 Some studies have reported that high concentrations of flavonoid contents of A. bracteolata inhibit cell proliferation of human fibroblast and HaCaT cell.…”

Section: Discussionmentioning

confidence: 99%

In vitro Wound Healing Potential and Antimicrobial Activity of Clerodendrum Inerme Leave Extracts

Wisessombat¹,

Tayeh²

2021

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Aristolochia bracteolata Enhances Wound Healing in vitro through Anti-inflammatory and Proliferative Effect on Human Dermal Fibroblasts and Keratinocytes

Cited by 14 publications

References 21 publications

In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression

In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

In vitro Wound Healing Potential and Antimicrobial Activity of Clerodendrum Inerme Leave Extracts

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